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The map label for this gene is gltX

Identifier: 126697617

GI number: 126697617

Start: 76997

End: 78478

Strand: Direct

Name: gltX

Synonym: CD0051

Alternate gene names: 126697617

Gene position: 76997-78478 (Clockwise)

Preceding gene: 126697616

Following gene: 126697618

Centisome position: 1.79

GC content: 30.36

Gene sequence:

>1482_bases
ATGAATGTAAGAGTAAGGTTTGCACCAAGTCCAACAGGATTTGTACATATAGGTAGTTTGAGAACTGCTTTATATAACTA
TTTATTTGCAAAAAAAATGGGCGGAGAATATATATTAAGAGTGGAAGATACAGACCAAAGTAGATTAGTAGAAGGTGCTA
TAGAGAATATGCTTAATGCTATGAAATGGGCTGGGGTCAACCATAGTGAAGGTGTTATATTAGATGATAGTGGAAAAGTA
GTACAAAAAGGAGAATATGGTCCATATATTCAATCTCAAAGATTAGATATATACCAAGAATATATAAAGCAACTTTTAGA
TAGTGGAAAAGCTTATTACTGTTTCTGTACAAAAGAAAGACTGGATGAAGTAAGAGATGCTCAAAGAGCAGCAGGAGAAA
CAGCTAAATATGATGGTCATTGTAAGAATCTTTCTAAAGAAGAAGTAGAAGCGAATATAAAAGCAGGAATACCATATGTT
ATAAGATTAAGATTGCCAGAGAATCATACAATTAAGTTTACAGACTTAGTTAGAGGAGATATGGAATTTAATACTAATGA
CTTAGATGACCAAGTATTAATGAAAACAGATGGATTTCCAACTTATCATTTTGCAGTAGTTGTTGATGACTATTTGATGA
AAATTACACATGTTATAAGAGGAGAAGAATGGGTATCATCTACTCCAAAACATGTTTATTTATATGAAGCTTTTGGATGG
GAAGCACCTGTATTTGTGCATTTACCAAATATACTTAATAAAGAAAAGAAAAAATTAAGTAAAAGACAAGGTGATGTAGC
TGTTGAAGACTTTAAGAAAAAAGGATATTTACCAGAGGGATTAGTAAACTATGTAGCATTAGTTGGTTGGTCTCCTGAAG
ATAATCAAGAATTGTTTACAATGGAGGAATTAGAAAAAGCCTTCTCTGTAGAAAGAGTCTCTAAAAGTGGTGGAGTATTT
GATACTGAAAAATTAAATTGGGTTAATCAGCATTATATAAAAGATGGTGATGATGCTTATCTAACAGACTTAGCAATACC
ATTTTTAATAGAAGATGGATTTATAACAGAAGAAGAAGCTACTAATAAATATGATTTCTTAAAGAGTATGATATCAGTAC
TTAAAGAGAAATTACAATATGTAAAAGAAGTTACTGAACATGCAAGTATATTTTTTGGAGATAAAATAGAGGTAGAAACT
GAAGAAGGAAGAGAGTTCTTAAGGTTAGAACATATACCTACTTTAATAGATGCTTTGAGAGAAAAAATAGAAAAAACAGA
GGTATTAAATGCTGAGTTTGTTCAAGCTATGCTTAAAGAAATACAAAAAGAGTACAAAATAAAAGGGAAAAATTTATTTA
TGGGCTCAAGAATAATTTTGACAGGGCAAATGCATGGTCCAGATTTACCAAAAGTAATGGAAGTTTTAGGAAAAGAAACT
TGCTTAAATAGAATAGCTTATGTAAAAAATAATATATTATAG

Upstream 100 bases:

>100_bases
AAGGATATTATTATTAGATTTGATTAAAAATAAACTTGCAAAACAATGTACTAGTTTAATATTATAGTTATATTGAATAA
AATATCAGGAGGAAGTAAAA

Downstream 100 bases:

>100_bases
TCATAAAAGTCCGCTAATTTAGAAGCGGACTTTTTTTGTGTTAGTATTTGGTGAAAATATCTGCTTAAGAAAATGAAGGT
ATTTAAGGTAATAAATTTAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDTDQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKV
VQKGEYGPYIQSQRLDIYQEYIKQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHVIRGEEWVSSTPKHVYLYEAFGW
EAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGYLPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVF
DTEKLNWVNQHYIKDGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFFGDKIEVET
EEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGKNLFMGSRIILTGQMHGPDLPKVMEVLGKET
CLNRIAYVKNNIL

Sequences:

>Translated_493_residues
MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDTDQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKV
VQKGEYGPYIQSQRLDIYQEYIKQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHVIRGEEWVSSTPKHVYLYEAFGW
EAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGYLPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVF
DTEKLNWVNQHYIKDGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFFGDKIEVET
EEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGKNLFMGSRIILTGQMHGPDLPKVMEVLGKET
CLNRIAYVKNNIL
>Mature_493_residues
MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDTDQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKV
VQKGEYGPYIQSQRLDIYQEYIKQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHVIRGEEWVSSTPKHVYLYEAFGW
EAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGYLPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVF
DTEKLNWVNQHYIKDGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFFGDKIEVET
EEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGKNLFMGSRIILTGQMHGPDLPKVMEVLGKET
CLNRIAYVKNNIL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=499, Percent_Identity=36.0721442885772, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=36.4197530864198, Blast_Score=334, Evalue=8e-93,
Organism=Escherichia coli, GI226510925, Length=300, Percent_Identity=32.6666666666667, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786895, Length=367, Percent_Identity=23.433242506812, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=35.2459016393443, Blast_Score=281, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=38.2293762575453, Blast_Score=343, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6321192, Length=316, Percent_Identity=25.3164556962025, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=37.525354969574, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CLOD6 (Q18CD6)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086514.1
- ProteinModelPortal:   Q18CD6
- SMR:   Q18CD6
- STRING:   Q18CD6
- GeneID:   4916663
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0051
- NMPDR:   fig|1496.1.peg.98
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MANKIRV
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56612; Mature: 56612

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDTDQSRLVEGAIENMLNA
CCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
MKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYIKQLLDSGKAYYCFCTKER
HHHCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYVIRLRLPENHTIKFTDLVRGD
HHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHCCC
MEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHVIRGEEWVSSTPKHVYLYEAFGW
CCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCC
EAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGYLPEGLVNYVALVGWSPEDNQELFT
CCCEEEECCHHHCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEE
MEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIKDGDDAYLTDLAIPFLIEDGFITEEEA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCHHC
TNKYDFLKSMISVLKEKLQYVKEVTEHASIFFGDKIEVETEEGREFLRLEHIPTLIDALR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEHHHCHHHHHHHHHHHHHHHHH
EKIEKTEVLNAEFVQAMLKEIQKEYKIKGKNLFMGSRIILTGQMHGPDLPKVMEVLGKET
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHH
CLNRIAYVKNNIL
HHHHHHHHHHCCC
>Mature Secondary Structure
MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDTDQSRLVEGAIENMLNA
CCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
MKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYIKQLLDSGKAYYCFCTKER
HHHCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYVIRLRLPENHTIKFTDLVRGD
HHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHCCC
MEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHVIRGEEWVSSTPKHVYLYEAFGW
CCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCC
EAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGYLPEGLVNYVALVGWSPEDNQELFT
CCCEEEECCHHHCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEE
MEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIKDGDDAYLTDLAIPFLIEDGFITEEEA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCHHC
TNKYDFLKSMISVLKEKLQYVKEVTEHASIFFGDKIEVETEEGREFLRLEHIPTLIDALR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEHHHCHHHHHHHHHHHHHHHHH
EKIEKTEVLNAEFVQAMLKEIQKEYKIKGKNLFMGSRIILTGQMHGPDLPKVMEVLGKET
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHH
CLNRIAYVKNNIL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA