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The map label for this gene is proS2

Identifier: 126697616

GI number: 126697616

Start: 75114

End: 76559

Strand: Direct

Name: proS2

Synonym: CD0050

Alternate gene names: 126697616

Gene position: 75114-76559 (Clockwise)

Preceding gene: 126697615

Following gene: 126697617

Centisome position: 1.75

GC content: 33.06

Gene sequence:

>1446_bases
ATGGCAAAAAATGAAAAACAATTTGTTGAAGAAATAACAAAGATGGAAGATGATTTTCCACAATGGTACACAGATGTAAT
AACTAAGACTGACTTAGTTGATTATGCACCAGTAAAAGGTTTTATGGTTGTAAAGCCATATGGATATGCATTATGGGAAA
AGATGCAAGAATTTATGGATAAAAAATTTAAAGAAACTGGACATAAAAACTGTTATTTTCCATTATTAATACCAGAGAGT
TTATTAAACAAAGAAGCAGAACACGTTGAAGGTTTTGCTCCAGAAGTTGCATGGGTAACACATGGAGGAAATAAAAAATT
AGAAGAAAGATTATGTGTAAGACCTACTTCAGAGACTATAATATGTACAATGTATGCGAAATGGTTAAAATCATATAGAG
AATTACCATATCTTTATAATCAATGGTGCTCAGTTGTTAGATGGGAAAAATCAACTAGACCTTTCTTAAGAACATCTGAG
TTTTTATGGCAAGAAGGTCATACGTTACATGAAACAGCAGAAGAAGCACAAGAAGAAACAATACAACAATTAGAAGTATA
TAAAGCGCTATGTGAAGAATTACTTGCAATGCCAGTTGTAGCTGGTCAAAAAAGTGAAAGTGAGAAATTTGCAGGTGGTG
AAAGAACATATACTATAGAAGCTATGATGCATGATGGTAAAGCATTACAATCAGGTACAAGTCACTTCCTAGGTCAACAC
TTTACTAAGGCTTTTGATATTACTTTTGCAGATAGGGAAGGAAATTTAGCTAATCCATACCATACATCTTGGGGAGCATC
TACAAGACTTATAGGTGGACTTATAATGACTCATAGTGATAACAGAGGTTTAGTATTACCACCAAGAGTAGCTCCAATTC
AAGTTGTTATAGTTCCTATAGCTGCTAAAAAAGGAAATGTAATGGAGACTGTTGATAAAATATATGCAGATTTAAAAGCT
AAGGGAGTAGCTGTAGAAGTTGATGATAGAGATAACTATACAACTGGATGGAAATTTAATGAGTGGGAAATGAAAGGTGT
TCCAGTAAGAGTTGAAATTGGACCAAAAGATATAGAAAATAATCAAGCTATGGTATTTAGAAGAGATACTTTAGAAAAGG
ATTCTATGCCTTTAGAAGGATTAGCTGATGCTATATGTGATTTATTTGATGTTATACACAATGATATGTTTGAAAAAGCA
AGAAAACATAGAGAAGACAATACATCTATAGTTGAAAATATGGATGAGTTTAGAAAAGCTTTAGAAGAAAAGCCAGGATT
CATAAAGACTATGTGGTGTGGAGATGCAGAATGTGAAGCTAAGATTAAGGAAGAAACAGGAGCAACTATAAGATGCTTAC
CTTTTGAACAAGAAAACTTAGGTCACAAGTGTGTGTACTGTGGAAAAGAAGCAGATAGTATGGTAGTAATGGCAAAAGCT
TACTAA

Upstream 100 bases:

>100_bases
TAGATAATCAAAGTTGTACTTTATAGACACCATTTTATCTATATATAAGGATATAAAATGGTGTTTTTTTATAAAACAAA
TAAATGAGGAGGTAATTAAA

Downstream 100 bases:

>100_bases
AATACATTTAAATAAAAACTGGGGTAGAAAGTATTTAATACTTTATCCCAGTTTTTTGTTTTTTATGAGTAATAAAATGT
ATTTGTAACTATGTCATAAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVVKPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPES
LLNKEAEHVEGFAPEVAWVTHGGNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYTIEAMMHDGKALQSGTSHFLGQH
FTKAFDITFADREGNLANPYHTSWGASTRLIGGLIMTHSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKA
KGVAVEVDDRDNYTTGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVIHNDMFEKA
RKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATIRCLPFEQENLGHKCVYCGKEADSMVVMAKA
Y

Sequences:

>Translated_481_residues
MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVVKPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPES
LLNKEAEHVEGFAPEVAWVTHGGNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYTIEAMMHDGKALQSGTSHFLGQH
FTKAFDITFADREGNLANPYHTSWGASTRLIGGLIMTHSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKA
KGVAVEVDDRDNYTTGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVIHNDMFEKA
RKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATIRCLPFEQENLGHKCVYCGKEADSMVVMAKA
Y
>Mature_480_residues
AKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVVKPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESL
LNKEAEHVEGFAPEVAWVTHGGNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSEF
LWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYTIEAMMHDGKALQSGTSHFLGQHF
TKAFDITFADREGNLANPYHTSWGASTRLIGGLIMTHSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAK
GVAVEVDDRDNYTTGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVIHNDMFEKAR
KHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATIRCLPFEQENLGHKCVYCGKEADSMVVMAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=496, Percent_Identity=42.9435483870968, Blast_Score=411, Evalue=1e-115,
Organism=Escherichia coli, GI1786392, Length=137, Percent_Identity=28.4671532846715, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=44.2857142857143, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=50, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321808, Length=497, Percent_Identity=43.8631790744467, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24649468, Length=478, Percent_Identity=43.3054393305439, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24649466, Length=478, Percent_Identity=43.3054393305439, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP2_CLOD6 (Q18CD7)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086513.1
- ProteinModelPortal:   Q18CD7
- SMR:   Q18CD7
- STRING:   Q18CD7
- GeneID:   4916662
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0050
- NMPDR:   fig|1496.1.peg.99
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 55146; Mature: 55015

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVVKPYGYALWEKMQEFMD
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
KKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHGGNKKLEERLCVRPTSETI
HHHHHCCCCCCEEEEECCHHHHCCHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCEE
ICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSEFLWQEGHTLHETAEEAQEET
EHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYTIEAMMHDGKALQSGTSHFLGQH
HHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCEEEEEEEEHHCCHHHHCCHHHHHHHH
FTKAFDITFADREGNLANPYHTSWGASTRLIGGLIMTHSDNRGLVLPPRVAPIQVVIVPI
HHHHEEEEEECCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEECCCCCCEEEEEEEE
AAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYTTGWKFNEWEMKGVPVRVEIGPKDIEN
ECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCEEECCEEEEEEECCCCCCC
NQAMVFRRDTLEKDSMPLEGLADAICDLFDVIHNDMFEKARKHREDNTSIVENMDEFRKA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEEKPGFIKTMWCGDAECEAKIKEETGATIRCLPFEQENLGHKCVYCGKEADSMVVMAKA
HHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEEC
Y
C
>Mature Secondary Structure 
AKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVVKPYGYALWEKMQEFMD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
KKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHGGNKKLEERLCVRPTSETI
HHHHHCCCCCCEEEEECCHHHHCCHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCEE
ICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSEFLWQEGHTLHETAEEAQEET
EHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYTIEAMMHDGKALQSGTSHFLGQH
HHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCEEEEEEEEHHCCHHHHCCHHHHHHHH
FTKAFDITFADREGNLANPYHTSWGASTRLIGGLIMTHSDNRGLVLPPRVAPIQVVIVPI
HHHHEEEEEECCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEECCCCCCEEEEEEEE
AAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYTTGWKFNEWEMKGVPVRVEIGPKDIEN
ECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCEEECCEEEEEEECCCCCCC
NQAMVFRRDTLEKDSMPLEGLADAICDLFDVIHNDMFEKARKHREDNTSIVENMDEFRKA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEEKPGFIKTMWCGDAECEAKIKEETGATIRCLPFEQENLGHKCVYCGKEADSMVVMAKA
HHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEEC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA