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The map label for this gene is proS

Identifier: 126697615

GI number: 126697615

Start: 73277

End: 74992

Strand: Direct

Name: proS

Synonym: CD0049

Alternate gene names: 126697615

Gene position: 73277-74992 (Clockwise)

Preceding gene: 126697614

Following gene: 126697616

Centisome position: 1.71

GC content: 32.58

Gene sequence:

>1716_bases
ATGAAAATGTCAAAAATGTTTATGCCGACATTAAAAGAAATCCCAGCAGATGCTGAGATTACAAGCCATCAATTAATGGT
TAGGTCAGGCATGATAAAAAAAATGACTTCAGGTGTTTATAACCAATTACCAATGGGGTTAAGAGTGTTCAAAAAAATTG
AACAAATAATTAGAGAAGAATTAAATAAAAAAGATTGTCAAGAAATACTTTGTGCAGCCTTACTTCCTTCTGAATTATGG
AAAGAGTCTGGAAGGTGGACTGCTATGGGTGAAGAAATGTTTAGACTAAAAGACAGAACAGAAAGAGAGTACTGTCTTGG
GCCAACACATGAAGAAGCATTCACTGATATAATAAGACAAGAAATTACTTCATATAAACAATTGCCACTAAACTTGTATC
AAATACAAGTAAAATATAGAGACGAGAGAAGACCAAGATTTGGTGTTATGAGAACTAAGACTTTTACAATGAAGGATGCA
TATAGTTTTGATGTAGATGATAAAGGTTTAGATAAGTCATATCAAGATATGTTTGATGCATATGTAAGTATATTTGATAG
ATGTGGATTGGAGAATAGCCCTGTACAGGCAGATTCAGGAGCTATAGGAGGTTCTACATCAGCCGAATTTATGGTTAAGT
CAGAAGTTGGTGAAGATGAAGTTGTATTTTGTAGTGGATGTGATTATGCAGCCAATGTAGAAAGAGCAGAATCTTGTAAT
CTAGCATCACAAAAAGAAGAAATGAAAGAATTAGAAGAGGTTCATACACCAGGAGCAGCTACAATAAAAGAGTTAGAAGA
ATTTTTAAAAACTTCTCCAGATAAGTTTGCTAAAACTTTAGTATATGAGGCAGATGGAAAAACAGTTGTAGTCGTTGTAA
GAGGAGATAGAGAAGTTAATGAAATCAAAGTATCTAATGCCATAGGTTCTGTTATAGAATTTGCTCTTGCTACAGATGAT
GTAGTGAGAAAAGTAACTAATGCAGAAGTAGGATTTGCAGGTCCAATAGGTATAAATGCTGACTATGTATTTATAGATAA
AGAAATTGTTGAACAAAGAAACATAGTTGTTGGGGCTAATAAAACTGAGTACCATATAAAAAATGCTAACTATGGAAGAG
ATTTTGAAGGAATAGTTGGAGACTTTAGAAATGTTCAAGAAGGCGACAAGTGTATAGTATGTGGTAAGCCACTTGAAATA
GCTAGAGGTGTTGAAGTAGGACATATATTTAAACTAGGAACTAAGTACTCAGAATCTATGAATGCAAACTTCATAGACAA
AGATGGAAAATCAAAGCCTATAGTAATGGGATGTTATGGAATAGGTGTTGAAAGAACAGCAGCAGCTATAATTGAACAAC
ATAATGATGAGAAAGGTATAATATGGCCTTTGTCAGTAGCTCCATACCATGTTGTGATAGTTCCAGCAAATATGAAAAAT
GAAGAACAAATATCAATAGCTGAAAATATATATAATGATTTGCAAGCTATGGGTGTAGAAGTTTTATTAGATGACAGAGA
TGAAAGAATAGGTGTCAAATTCAATGATTCAGAATTAATAGGAATACCTATGAGGATTACAGTAGGTAAAAATATTAATG
AAGGTAAAGTAGAATTTAAACTTAGACATAAAGAAGATAAAGAGATAATTGACATAGAAGAAATAAATGAAAAAGTAAAG
GCAGAGTTTATCAGGAATAATGTTAGGTTAGGATAA

Upstream 100 bases:

>100_bases
GAGTGGTACCGCGGAAATTAAACCTTTCGTCTCTATACTTGTATAGAGATGAGAGGTTTTTTATATTTTCAGGAAGTTAA
TATTCAGGAGGAATCTATTA

Downstream 100 bases:

>100_bases
TAACTTAAATAAGATATTGTATAGATAATCAAAGTTGTACTTTATAGACACCATTTTATCTATATATAAGGATATAAAAT
GGTGTTTTTTTATAAAACAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 1; ProRS 1

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLPMGLRVFKKIEQIIREELNKKDCQEILCAALLPSELW
KESGRWTAMGEEMFRLKDRTEREYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGEDEVVFCSGCDYAANVERAESCN
LASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKFAKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDD
VVRKVTNAEVGFAGPIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCIVCGKPLEI
ARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAIIEQHNDEKGIIWPLSVAPYHVVIVPANMKN
EEQISIAENIYNDLQAMGVEVLLDDRDERIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVK
AEFIRNNVRLG

Sequences:

>Translated_571_residues
MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLPMGLRVFKKIEQIIREELNKKDCQEILCAALLPSELW
KESGRWTAMGEEMFRLKDRTEREYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGEDEVVFCSGCDYAANVERAESCN
LASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKFAKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDD
VVRKVTNAEVGFAGPIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCIVCGKPLEI
ARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAIIEQHNDEKGIIWPLSVAPYHVVIVPANMKN
EEQISIAENIYNDLQAMGVEVLLDDRDERIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVK
AEFIRNNVRLG
>Mature_571_residues
MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLPMGLRVFKKIEQIIREELNKKDCQEILCAALLPSELW
KESGRWTAMGEEMFRLKDRTEREYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGEDEVVFCSGCDYAANVERAESCN
LASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKFAKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDD
VVRKVTNAEVGFAGPIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCIVCGKPLEI
ARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAIIEQHNDEKGIIWPLSVAPYHVVIVPANMKN
EEQISIAENIYNDLQAMGVEVLLDDRDERIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVK
AEFIRNNVRLG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.9203539823009, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1786392, Length=565, Percent_Identity=47.0796460176991, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1788013, Length=119, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=247, Percent_Identity=36.4372469635627, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71991342, Length=189, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203271, Length=108, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71991346, Length=158, Percent_Identity=31.0126582278481, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=523, Percent_Identity=31.1663479923518, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656200, Length=269, Percent_Identity=35.3159851301115, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP1_CLOD6 (Q18CD2)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086512.1
- ProteinModelPortal:   Q18CD2
- SMR:   Q18CD2
- STRING:   Q18CD2
- GeneID:   4916638
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0049
- NMPDR:   fig|1496.1.peg.100
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64400; Mature: 64400

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLPMGLRVFKKIEQIIREE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTEREYCLGPTHEEAFTDIIRQ
CCHHHHHHHHHHHHCHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDAYSFDVDDKGLDKSYQDMFDA
HHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCEECCCCCCCCCHHHHHHHHH
YVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGEDEVVFCSGCDYAANVERAESCN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHCCC
LASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKFAKTLVYEADGKTVVVVVRGDREVN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHEEEECCCCEEEEEEECCCCCH
EIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAGPIGINADYVFIDKEIVEQRNIVVGAN
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHCCEEEECC
KTEYHIKNANYGRDFEGIVGDFRNVQEGDKCIVCGKPLEIARGVEVGHIFKLGTKYSESM
CCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHHHEEECCCHHHHCC
NANFIDKDGKSKPIVMGCYGIGVERTAAAIIEQHNDEKGIIWPLSVAPYHVVIVPANMKN
CCCEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCC
EEQISIAENIYNDLQAMGVEVLLDDRDERIGVKFNDSELIGIPMRITVGKNINEGKVEFK
CHHHHHHHHHHHHHHHCCEEEEEECCCCEECEEECCCCEEEEEEEEEECCCCCCCEEEEE
LRHKEDKEIIDIEEINEKVKAEFIRNNVRLG
EECCCCCCEECHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLPMGLRVFKKIEQIIREE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTEREYCLGPTHEEAFTDIIRQ
CCHHHHHHHHHHHHCHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDAYSFDVDDKGLDKSYQDMFDA
HHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCEECCCCCCCCCHHHHHHHHH
YVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGEDEVVFCSGCDYAANVERAESCN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHCCC
LASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKFAKTLVYEADGKTVVVVVRGDREVN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHEEEECCCCEEEEEEECCCCCH
EIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAGPIGINADYVFIDKEIVEQRNIVVGAN
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHCCEEEECC
KTEYHIKNANYGRDFEGIVGDFRNVQEGDKCIVCGKPLEIARGVEVGHIFKLGTKYSESM
CCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHHHEEECCCHHHHCC
NANFIDKDGKSKPIVMGCYGIGVERTAAAIIEQHNDEKGIIWPLSVAPYHVVIVPANMKN
CCCEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCC
EEQISIAENIYNDLQAMGVEVLLDDRDERIGVKFNDSELIGIPMRITVGKNINEGKVEFK
CHHHHHHHHHHHHHHHCCEEEEEECCCCEECEEECCCCEEEEEEEEEECCCCCCCEEEEE
LRHKEDKEIIDIEEINEKVKAEFIRNNVRLG
EECCCCCCEECHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA