Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 126697614

GI number: 126697614

Start: 72425

End: 72910

Strand: Direct

Name: ispF

Synonym: CD0048

Alternate gene names: 126697614

Gene position: 72425-72910 (Clockwise)

Preceding gene: 126697613

Following gene: 126697615

Centisome position: 1.69

GC content: 31.89

Gene sequence:

>486_bases
ATGAGAATAGGATTAGGATATGATGTACACAAATTAACAGAGGATAGAAAACTTATAATCGGTGGTGTAGAGATACCACA
CGATAAAGGTTTGTTGGGTCATTCTGATGCTGATGTATTGATACATGCAATAATGGATTCTATTTTAGGTGCTCTAGCAT
TAGGAGATATAGGGAAGCATTTCCCAGATACAGATGAAGAATATAAGGGAGCAGATAGTATGAAATTATTAGAACATGTG
TATAATCTAATAACAAGTAAAGGATATAAAATTGGTAATATAGACAGTACTATAATTGCTCAAAGCCCTAAGATGGCTCC
ATATATTGAGAGTATGAGAAGTAATATATCAAAAGTTTTAAACACAGACATTGATAATATAAACATAAAGGCAACAACAG
AAGAAGGTCTTGGTTTTACAGGAGCAAAACAGGGAATAGCTTCACAAAGTATTTGTTTATTATTGTTGACAAGTCAGAAT
AATTAA

Upstream 100 bases:

>100_bases
ATGAAAACATAAAAATAACTAGTCCAGAAGATTTAAATATAGCAGAACAAATATTAAATATGGAAAAAAGAGATGAAGTT
AACAGTAGAAGGAGAATTAT

Downstream 100 bases:

>100_bases
TATATTATTAGTAATAAGTTTAATGGATATCAAGTAATAGGCCTATATAGAATATCTATAATGTATGAATTTAAGTGAAA
TTAATATAAATATAAAATTA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAIMDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHV
YNLITSKGYKIGNIDSTIIAQSPKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
N

Sequences:

>Translated_161_residues
MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAIMDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHV
YNLITSKGYKIGNIDSTIIAQSPKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
N
>Mature_161_residues
MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAIMDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHV
YNLITSKGYKIGNIDSTIIAQSPKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
N

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=54.7169811320755, Blast_Score=175, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CLOD6 (Q18CD3)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086511.1
- ProteinModelPortal:   Q18CD3
- SMR:   Q18CD3
- STRING:   Q18CD3
- GeneID:   4916636
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0048
- NMPDR:   fig|1496.1.peg.101
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   CLSK2534352
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17434; Mature: 17434

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAIMDSILGALALGDIGKH
CCCCCCCCHHHHCCCCEEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQSPKMAPYIESMRSNISKVL
CCCCCHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHH
NTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQNN
HCCCCCCEEEEECCCCCCCCCHHHCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAIMDSILGALALGDIGKH
CCCCCCCCHHHHCCCCEEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQSPKMAPYIESMRSNISKVL
CCCCCHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHH
NTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQNN
HCCCCCCEEEEECCCCCCCCCHHHCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA