Click here to switch to the map view.

The map label for this gene is lplA [H]

Identifier: 126697612

GI number: 126697612

Start: 70509

End: 71441

Strand: Direct

Name: lplA [H]

Synonym: CD0046

Alternate gene names: 126697612

Gene position: 70509-71441 (Clockwise)

Preceding gene: 126697611

Following gene: 126697613

Centisome position: 1.64

GC content: 23.69

Gene sequence:

>933_bases
ATGTATTACTTATTAAACAAAAGCACAAATCCTTACTTTAATTTGGCATTAGAAGAATATCTATTTTTAAATGATAAATA
TAATGATGATATTATAATCATATGGAGAAATGAAGAATCCATTTTTATAGGTAAAAATCAAAATCCATATCAGGAAGTAT
ATCATGATGTTATTGAAAAGGGAGAAATTCCTATTTTAAGAAGAATATCTGGAGGAGGAACTGTTTATCATGACTTAGGT
AATATCAATATGTCATTCATACAAAAAGATAGGCAACTTCATGAGATAGATTTTTTGGAGCATACTAAGTTTATGCAAAA
GATGCTTTCAACCTTAGGACTAGATGTATCTATAACTGAAAGAAAAGATTTATTTTTAAATGGGAAAAAAATTTCAGGTT
CTGCACAGTCTATAAAAAGAAAAAATTCTCTTTATCATGGAACTCTTCTTTACGATTCAGATTTAAATAAATTAACTAAA
TATTTAAATTCAAATAAAGCAACTGAATCAAATGCAACTAAATCTGTTTCAAGTAAAGTTACAAATATTAAGTTTTTACT
CGAAAAAGATATAAATTACTTTTTAGATTATTGTGTTGAATATTTAAAATCTAATATAAAGAATATAAAAGAACTTGAAT
TGGATGAAGAAGATATTAAAAGTGTATACAATATAGGAGAAGATAAATATAAAAAGATAGAGTGGACATATGGTAAAACG
CCGAAATTTCAAGTAGTGCTACCATTTAATAATGAAAGTAAAATAAATATACATGTTAACAGATGGAAAGTATCAAAATT
TTTTATTTCTTATGATGATAATGTAATTAATATGGACAAGTTGTTAGATTTGAATTTCTTTAAAGAAGAAATAAAAGAGA
GTATTTTAGAAAATTATCCACATTACATAGATTTAATCAATTTAATATTTTAA

Upstream 100 bases:

>100_bases
TAAGTTTAATAGTTAAGAAGTAAAATATGAAATGTAATAGTATATAAATTCATTTAAGACTAAAAATAATAAGATAAAAT
AAAAAGGAGTCTGTATTTCT

Downstream 100 bases:

>100_bases
TATAAAACCTCTAATTTGATAAAATTGTCTTATATCAAACTAGAGGTTTTTAATATTCTTGCATATTAACAACCATTACA
AAATTGTAAAATTAAAAACA

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIGKNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLG
NINMSFIQKDRQLHEIDFLEHTKFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEEDIKSVYNIGEDKYKKIEWTYGKT
PKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVINMDKLLDLNFFKEEIKESILENYPHYIDLINLIF

Sequences:

>Translated_310_residues
MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIGKNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLG
NINMSFIQKDRQLHEIDFLEHTKFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEEDIKSVYNIGEDKYKKIEWTYGKT
PKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVINMDKLLDLNFFKEEIKESILENYPHYIDLINLIF
>Mature_310_residues
MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIGKNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLG
NINMSFIQKDRQLHEIDFLEHTKFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEEDIKSVYNIGEDKYKKIEWTYGKT
PKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVINMDKLLDLNFFKEEIKESILENYPHYIDLINLIF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=257, Percent_Identity=35.0194552529183, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI21729884, Length=257, Percent_Identity=35.0194552529183, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI21729882, Length=257, Percent_Identity=35.0194552529183, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI21729880, Length=257, Percent_Identity=35.0194552529183, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI21729878, Length=257, Percent_Identity=35.0194552529183, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790846, Length=263, Percent_Identity=30.0380228136882, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71981909, Length=260, Percent_Identity=36.1538461538462, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=257, Percent_Identity=34.2412451361868, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573464, Length=268, Percent_Identity=29.8507462686567, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 36639; Mature: 36639

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIGKNQNPYQEVYHDVIEK
CEEEEECCCCCEEEEEEEHEEEECCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHC
GEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHTKFMQKMLSTLGLDVSITE
CCCCEEEEECCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
RKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTKYLNSNKATESNATKSVSSKV
CCCEEEECCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHH
TNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEEDIKSVYNIGEDKYKKIEWTYGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHEEEEEECCCC
PKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVINMDKLLDLNFFKEEIKESILENYP
CCEEEEEECCCCCEEEEEEEEEEEEEEEEEECCCEECHHHHHHHHHHHHHHHHHHHHCCC
HYIDLINLIF
HHHHHHHHHC
>Mature Secondary Structure
MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIGKNQNPYQEVYHDVIEK
CEEEEECCCCCEEEEEEEHEEEECCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHC
GEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHTKFMQKMLSTLGLDVSITE
CCCCEEEEECCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
RKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTKYLNSNKATESNATKSVSSKV
CCCEEEECCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHH
TNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEEDIKSVYNIGEDKYKKIEWTYGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHEEEEEECCCC
PKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVINMDKLLDLNFFKEEIKESILENYP
CCEEEEEECCCCCEEEEEEEEEEEEEEEEEECCCEECHHHHHHHHHHHHHHHHHHHHCCC
HYIDLINLIF
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]