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The map label for this gene is radA [H]

Identifier: 126697593

GI number: 126697593

Start: 45541

End: 46914

Strand: Direct

Name: radA [H]

Synonym: CD0027

Alternate gene names: 126697593

Gene position: 45541-46914 (Clockwise)

Preceding gene: 126697592

Following gene: 126697594

Centisome position: 1.06

GC content: 31.44

Gene sequence:

>1374_bases
ATGGCAAAAATAAAAACAAAATATGTATGTCAATCTTGCGGATATGAAACAGCTAAATGGCTTGGTAAATGTCCAGAATG
TACAAAATGGAATACATTTGTAGAAGAAATAGACCAAAAAAGCACCAAAAAAGAGGTCTTTATAATAGATAAATCTTCAT
CTAAACCAGTCAGTATAAACTCAATAGAGAGTAAAGAAGAAGAGCGGTTTACAACAGATATAAATGAATTAGATAGAGTG
CTTGGAGGAGGTATAGTTAAAGGCTCTTTAGTGCTTGTTGGTGGTGACCCTGGTATAGGTAAATCAACACTTTTAATCCA
AGTATCTAGTAATGTTGCCAACTTAGGAAAAACTGTTTTATATATAACTGGAGAAGAATCAGAATCTCAAATAAAAATGA
GAGCTAAAAGATTAGGTATTAACTCAGAAAATTTATATATATTTGCTGAAAATAATTTAAGTATAATTGAATCTTATTTA
GAAAGTGTAAATCCAGAATTAATAATTCTAGACTCAATTCAAACTGTCTTCAGTCCAGAAATATCCTCGGCACCAGGAAC
TGTCAGCCAAATTAAAGAAGGTACTTCAAAATTCATGAAAATTTCTAAAAAAATGGGAATTTCAACGTTCATAGTTGGGC
ATGTTACAAAAGAAGGTTCACTAGCAGGACCAAAATTATTAGAGCATATGGTTGATACTGTTCTATATTTTGAAGGAGAA
AGATATAATACTTATAGATTAGTAAGAGCTGTTAAAAATCGATTTGGTTCAACTAATGAACTTGGAGTGTTTGAAATGAG
GGATTTAGGATTAGTAGAACTTGATAATCCATCTAAAATTTTAATCTCTGAAAAACCAAAAGATGTAGCAGGTTCAGTAA
TAATATCTACTGTAGAAGGTACGAGACCAATGTTACTTGAATTGCAAGCATTAGTTTCTCCTACAAGTTTTGGTATACCA
AAAAGAACATCTACAGGAGTCGATTACAATAGAGTAGGTATGTTATTAGCAGTATTAGAAAAACGTGTTGGTTTGCAAAT
ACAAAATCAAGATGTCTATATAAACATAGTTGGAGGAATAAAGATAAATGAACCCTCAATTGATTTAGGGATAGCCATTT
CTGTAGCATCTAGTTTTAGAAATATACCGATAGATGAAGATATAGCTGTTACAGGAGAAGTGGGACTTACAGGAGAAGTT
AGAGCAGTAAGCTTTATAGAAAAAAGAATAGCTGAGTGTAAAAAACTTGGATTTAAAAAGATAGTAGTTCCTAGAAGTAA
TTATGATGTTGTAAAAGAGACCAAAGGCATAGAAATATGGCCAGTAGATAACTTAAGACAAGCTATAAATATAGTACTTG
GGAGGAATCAATAA

Upstream 100 bases:

>100_bases
ATAATTCATTGTCCTTAATTATTTTATTTAATCTTTACATATTGTCTTTCAGATATGCTAAAATTATAGTATAGAATAAA
AAATTAAGGATGATAAAAGT

Downstream 100 bases:

>100_bases
ATATGGAGAATTTTCTAGATAATAAAAATATGCTATATGCATTAAAAATGATATCTCCTGGAACTCCACTTAGATTAGGT
CTAAACAATGTACTAAGAGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSINSIESKEEERFTTDINELDRV
LGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIP
KRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEV
RAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ

Sequences:

>Translated_457_residues
MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSINSIESKEEERFTTDINELDRV
LGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIP
KRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEV
RAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ
>Mature_456_residues
AKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVL
GGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYLE
SVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGER
YNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPK
RTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEVR
AVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=458, Percent_Identity=41.2663755458515, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50275; Mature: 50144

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSIN
CCCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
SIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVL
CCCCCHHHHHHCCHHHHHHHHCCCEEECEEEEEECCCCCCCEEEEEEECCCHHCCCCEEE
YITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYLESVNPELIILDSIQTVFSPE
EEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECHHHHHCCC
ISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCC
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEG
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCEEEEEECCC
TRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGI
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEEEEEECE
KINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEVRAVSFIEKRIAECKKLGFKK
EECCCCCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEE
IVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ
EEECCCCCCHHHCCCCEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSIN
CCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
SIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVL
CCCCCHHHHHHCCHHHHHHHHCCCEEECEEEEEECCCCCCCEEEEEEECCCHHCCCCEEE
YITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYLESVNPELIILDSIQTVFSPE
EEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECHHHHHCCC
ISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCC
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEG
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCEEEEEECCC
TRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGI
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEEEEEECE
KINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEVRAVSFIEKRIAECKKLGFKK
EECCCCCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEE
IVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ
EEECCCCCCHHHCCCCEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]