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The map label for this gene is hpaG [H]

Identifier: 126447956

GI number: 126447956

Start: 1467001

End: 1467699

Strand: Reverse

Name: hpaG [H]

Synonym: BMA10247_A1513

Alternate gene names: 126447956

Gene position: 1467699-1467001 (Counterclockwise)

Preceding gene: 126446667

Following gene: 126446097

Centisome position: 62.38

GC content: 70.82

Gene sequence:

>699_bases
ATGAATCGCATGGAGCTGTCTCTCGTCGAACGCGGCGAGCGCGTCGCCACGGTGCCGATCGGCACGGTCTACGGCGCGCT
GCTCAACGATCGCGGCGCGCTCGCCGCGCTGGGCGACGCGGTGCACGCGCCGCCGTACCACAAGCCGCCGCGCGCGTCCG
TGCTGTATGTGAAGCCGGCCAACACGCACGCGGGCGACGGCGCGGCCGTGGTCGTGCCGGCGGGCGTCGACGCGCTCGAA
ATCGGCGCGACGCTCGGCGTCGTGTTCGCCCGCCGCACGACGCGCGTGCCGGCCGAACAGGCGTTTGATTTTGTTAAAGG
TTTTACGCTTGCCAGCGACGTGTCGATTCCCCATCCTGATTATTACCGGCCGGCGGTGCGCTTCAAGTGCCGCGATGGCT
TCTGCCCGCTCGGGCCGGCGATCGTTGCGCGCGCAAGCATCGGCGACGCGGACGCGCTCGCCGTGACGGTGCGCATCGAC
GGCGAGCGGCGCTACGAGGCGAGCACCGCGAACCTGATTCGCCCGGTGGCGCGGCTCATCGCCGACGTGTCGGCGTTCAT
GTCGTTCGACGCGGGCGACGTGCTGCTCGTCGGCGTCGGCCCGAACGCGCCGCGCGCGGCGGCGGGCAGCACGATCGAGA
TCGACGCGCCGGGCATCGGCACGCTGCGTCATACATTGGTGGCGGAGGACGCGCAATGA

Upstream 100 bases:

>100_bases
GACCGCCTTATCAGGGTTATCAGGGTTATTGCGGTTGTTCTTTTCGCGCGCTGTAGTTAATATGTTAAGTAATTGACCCG
GCCGCGTGACGCGGCGGAGG

Downstream 100 bases:

>100_bases
GGACCGCACGCGTAGTGTTCAACGGCGCACTGCATGCGGCCGAGCCGGCGGGCGACGCGCTCGTGCGGCTCGATACCGGG
CGCGTGCTCGCCGAGACGGA

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNRMELSLVERGERVATVPIGTVYGALLNDRGALAALGDAVHAPPYHKPPRASVLYVKPANTHAGDGAAVVVPAGVDALE
IGATLGVVFARRTTRVPAEQAFDFVKGFTLASDVSIPHPDYYRPAVRFKCRDGFCPLGPAIVARASIGDADALAVTVRID
GERRYEASTANLIRPVARLIADVSAFMSFDAGDVLLVGVGPNAPRAAAGSTIEIDAPGIGTLRHTLVAEDAQ

Sequences:

>Translated_232_residues
MNRMELSLVERGERVATVPIGTVYGALLNDRGALAALGDAVHAPPYHKPPRASVLYVKPANTHAGDGAAVVVPAGVDALE
IGATLGVVFARRTTRVPAEQAFDFVKGFTLASDVSIPHPDYYRPAVRFKCRDGFCPLGPAIVARASIGDADALAVTVRID
GERRYEASTANLIRPVARLIADVSAFMSFDAGDVLLVGVGPNAPRAAAGSTIEIDAPGIGTLRHTLVAEDAQ
>Mature_232_residues
MNRMELSLVERGERVATVPIGTVYGALLNDRGALAALGDAVHAPPYHKPPRASVLYVKPANTHAGDGAAVVVPAGVDALE
IGATLGVVFARRTTRVPAEQAFDFVKGFTLASDVSIPHPDYYRPAVRFKCRDGFCPLGPAIVARASIGDADALAVTVRID
GERRYEASTANLIRPVARLIADVSAFMSFDAGDVLLVGVGPNAPRAAAGSTIEIDAPGIGTLRHTLVAEDAQ

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=155, Percent_Identity=32.9032258064516, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI40786394, Length=155, Percent_Identity=32.9032258064516, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI66348062, Length=183, Percent_Identity=31.1475409836066, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI215422413, Length=157, Percent_Identity=31.2101910828025, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI13654274, Length=157, Percent_Identity=31.2101910828025, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787428, Length=161, Percent_Identity=26.0869565217391, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17557057, Length=170, Percent_Identity=25.8823529411765, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28572127, Length=214, Percent_Identity=26.1682242990654, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24663695, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 24248; Mature: 24248

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMELSLVERGERVATVPIGTVYGALLNDRGALAALGDAVHAPPYHKPPRASVLYVKPA
CCCEEEHHHHCCCEEEEEEHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCCCEEEEEECC
NTHAGDGAAVVVPAGVDALEIGATLGVVFARRTTRVPAEQAFDFVKGFTLASDVSIPHPD
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCCCCCC
YYRPAVRFKCRDGFCPLGPAIVARASIGDADALAVTVRIDGERRYEASTANLIRPVARLI
CCCCCEEEEECCCCCCCCHHHEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
ADVSAFMSFDAGDVLLVGVGPNAPRAAAGSTIEIDAPGIGTLRHTLVAEDAQ
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHEEEECCCC
>Mature Secondary Structure
MNRMELSLVERGERVATVPIGTVYGALLNDRGALAALGDAVHAPPYHKPPRASVLYVKPA
CCCEEEHHHHCCCEEEEEEHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCCCEEEEEECC
NTHAGDGAAVVVPAGVDALEIGATLGVVFARRTTRVPAEQAFDFVKGFTLASDVSIPHPD
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCCCCCC
YYRPAVRFKCRDGFCPLGPAIVARASIGDADALAVTVRIDGERRYEASTANLIRPVARLI
CCCCCEEEEECCCCCCCCHHHEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
ADVSAFMSFDAGDVLLVGVGPNAPRAAAGSTIEIDAPGIGTLRHTLVAEDAQ
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA