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The map label for this gene is benB [H]

Identifier: 126447624

GI number: 126447624

Start: 181823

End: 182314

Strand: Reverse

Name: benB [H]

Synonym: BMA10247_A0214

Alternate gene names: 126447624

Gene position: 182314-181823 (Counterclockwise)

Preceding gene: 126446977

Following gene: 126445721

Centisome position: 7.75

GC content: 61.38

Gene sequence:

>492_bases
ATGAAGACGATCGATCTCGCCGACATCCAGGCGTTCCTCTATCGCGAGAGCCGGCTGCTCGACGACAAGGCGTGGGACGC
ATGGCTCGACTGCTATCGCGCGGACGCCGTGTTCTGGATGCCTTCCTGGGACGACGCGGATGCGCTCGTCACCGATCCGC
AGCGCGAAATCTCGCTGATCTACTACCCGAACCGGCAGGGGCTCGAGGATCGCGTGTTCCGCATCAAGACCGAGCGCTCG
AGCGCGACGGTGCCCGATACGCGCACGAGCCACAACATCGCGAACGTCGAGCGCGAAAGCGCCGATGGCGACGTGCACAC
CGTGCGCTTCAACTGGCACACGCTCAGCTATCGCTACAAGACGGTGTCGAGCTATTTCGGCATGTCGCGCTACGCGATCG
ATTTCTCCGGCGACGCGCCGAAGATCGTCAGCAAGTACGTCGTCCTGAAGAACGACTACATCAACCAGCTGATCGACATC
TACCACATCTAG

Upstream 100 bases:

>100_bases
TGAGCGGCGTGAAGACCGAGGACGAAGGGCTGTACACGGTCCAGCACCGCTACTGGATCGCGACGATGAAGCAGGCGCTC
GCCGCCGAAAGGAGCGGCGC

Downstream 100 bases:

>100_bases
CCGGTGCGCGCCGCGGCAGCGCGGCCCGCTCGAGGAACGCATCATGGAACACCGCATCGCCCTGCAGTTCGAAGACGGCG
TCACCCGCTTCATCGCCTGC

Product: benzoate 1,2-dioxygenase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKTIDLADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERS
SATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDI
YHI

Sequences:

>Translated_163_residues
MKTIDLADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERS
SATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDI
YHI
>Mature_163_residues
MKTIDLADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERS
SATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDI
YHI

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 19132; Mature: 19132

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIDLADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLI
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCEEEE
YYPNRQGLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYK
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEE
TVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI
HHHHHCCCEEEEEEECCCCHHHHHHEEEECHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIDLADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLI
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCEEEE
YYPNRQGLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYK
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEE
TVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI
HHHHHCCCEEEEEEECCCCHHHHHHEEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]