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The map label for this gene is benA [H]

Identifier: 126446977

GI number: 126446977

Start: 182311

End: 183678

Strand: Reverse

Name: benA [H]

Synonym: BMA10247_A0215

Alternate gene names: 126446977

Gene position: 183678-182311 (Counterclockwise)

Preceding gene: 126445801

Following gene: 126447624

Centisome position: 7.81

GC content: 66.89

Gene sequence:

>1368_bases
ATGATCCCGATCGACCCGGACCGCCGTGCGACGCCGCGCCCCATCGACGATTTCCTCGTCGAAGACAAGGCGCGCGGCGA
CTACCGGCTGCACCGCAGCGCGTTCACCGACGAAATGCTGTTCGAGCTCGAGATGAAGCACATCTTCGAAGGCAACTGGA
TCTATCTCGCGCACGAGAGCCAGCTCCCGAACGCGAACGATTACTACACGACCACGATCGGCCGCCAGCCGATCGTGATC
GCGCGCAACCGCCACGGCGAGCTGAACGCGTTCGTCAACGCCTGCACGCACCGCGGCGCGATGCTGTGCCGCCACAAGCG
CGGCAACCGCGCGAGCTACACGTGCCCGTTCCACGGCTGGACGTTCAGCAACGGCGGCAAGCTGCTCAAAGTGAAGGACC
CCGAAGGAGCCGGCTACCCGGACTGCTTCAACCGCGACGGCTCGCACGATCTGAAGAAAGTCGCGCGCTTCGAGAACTAT
CGCGGCTTCCTGTTCGGCAGCCTGAACCCCGAAGTCGAGCCGCTCGCCGCGCATCTCGGCGATGCCGCGCGCATCATCGA
CATGATCGTCGATCAGTCGGCGGACGGCCTCGAGGTGCTGCGCGGCTCGTCGACGTACACGTACGAAGGCAACTGGAAGC
TCACCGCCGAGAACGGCGCGGACGGCTACCACGTATCGGCCGTTCACTGGAACTACGCGGCGACCGTCAACCACCGCAAG
ACCGACGCGCAGCACGAAGACACGATCCGCGCGATGGACGCGGGCAACTGGGGCCGGCAGGGCGGCGGCTTCTACGCGTT
CGATCACGGCCACATGCTGCTGTGGACGCGCTGGGCGAACCCGGAGGACCGGCCGAACTTCGATCGCCGCGACGAATTCG
CCGCGCGCTGCGGCGGCGACGTCGCCGACTGGATGATCCGGAACTCGCGCAACCTGTGCCTGTACCCGAACGTCTATCTG
ATGGACCAGTTCGGCTCGCAGATCCGCGTGCTGCGCCCGCTCGCCGTCGATCGCACCGAGGTCACGATCTACTGCATCGC
GCCGAAGGGCGAGGCGCCCGACGCGCGCGCGCGGCGCATCCGCCAGTACGAAGATTTCTTCAACGTGAGCGGAATGGCGA
CGCCCGACGATCTCGAGGAATTCCGCGCGTGCCAGCAGGGCTACGCGGGCCGCGCGGTCGAATGGAACGACATGTGCCGC
GGCGCCTCGCACTGGATCGAGGGCCCCGACGAAGCGGCGCGCCGGATCGGCATCCGCCCGCTGATGAGCGGCGTGAAGAC
CGAGGACGAAGGGCTGTACACGGTCCAGCACCGCTACTGGATCGCGACGATGAAGCAGGCGCTCGCCGCCGAAAGGAGCG
GCGCATGA

Upstream 100 bases:

>100_bases
ACCCGGGGTTTCCCATGCGACGCGCGGCGCGGCGCCTCGCCGGACCGCGCGTCCGCACGCGCGCCGCCCCCGATCAGAGA
GCCCATCAGGAGACGCCCCC

Downstream 100 bases:

>100_bases
AGACGATCGATCTCGCCGACATCCAGGCGTTCCTCTATCGCGAGAGCCGGCTGCTCGACGACAAGGCGTGGGACGCATGG
CTCGACTGCTATCGCGCGGA

Product: benzoate 1,2-dioxygenase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIPIDPDRRATPRPIDDFLVEDKARGDYRLHRSAFTDEMLFELEMKHIFEGNWIYLAHESQLPNANDYYTTTIGRQPIVI
ARNRHGELNAFVNACTHRGAMLCRHKRGNRASYTCPFHGWTFSNGGKLLKVKDPEGAGYPDCFNRDGSHDLKKVARFENY
RGFLFGSLNPEVEPLAAHLGDAARIIDMIVDQSADGLEVLRGSSTYTYEGNWKLTAENGADGYHVSAVHWNYAATVNHRK
TDAQHEDTIRAMDAGNWGRQGGGFYAFDHGHMLLWTRWANPEDRPNFDRRDEFAARCGGDVADWMIRNSRNLCLYPNVYL
MDQFGSQIRVLRPLAVDRTEVTIYCIAPKGEAPDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGRAVEWNDMCR
GASHWIEGPDEAARRIGIRPLMSGVKTEDEGLYTVQHRYWIATMKQALAAERSGA

Sequences:

>Translated_455_residues
MIPIDPDRRATPRPIDDFLVEDKARGDYRLHRSAFTDEMLFELEMKHIFEGNWIYLAHESQLPNANDYYTTTIGRQPIVI
ARNRHGELNAFVNACTHRGAMLCRHKRGNRASYTCPFHGWTFSNGGKLLKVKDPEGAGYPDCFNRDGSHDLKKVARFENY
RGFLFGSLNPEVEPLAAHLGDAARIIDMIVDQSADGLEVLRGSSTYTYEGNWKLTAENGADGYHVSAVHWNYAATVNHRK
TDAQHEDTIRAMDAGNWGRQGGGFYAFDHGHMLLWTRWANPEDRPNFDRRDEFAARCGGDVADWMIRNSRNLCLYPNVYL
MDQFGSQIRVLRPLAVDRTEVTIYCIAPKGEAPDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGRAVEWNDMCR
GASHWIEGPDEAARRIGIRPLMSGVKTEDEGLYTVQHRYWIATMKQALAAERSGA
>Mature_455_residues
MIPIDPDRRATPRPIDDFLVEDKARGDYRLHRSAFTDEMLFELEMKHIFEGNWIYLAHESQLPNANDYYTTTIGRQPIVI
ARNRHGELNAFVNACTHRGAMLCRHKRGNRASYTCPFHGWTFSNGGKLLKVKDPEGAGYPDCFNRDGSHDLKKVARFENY
RGFLFGSLNPEVEPLAAHLGDAARIIDMIVDQSADGLEVLRGSSTYTYEGNWKLTAENGADGYHVSAVHWNYAATVNHRK
TDAQHEDTIRAMDAGNWGRQGGGFYAFDHGHMLLWTRWANPEDRPNFDRRDEFAARCGGDVADWMIRNSRNLCLYPNVYL
MDQFGSQIRVLRPLAVDRTEVTIYCIAPKGEAPDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGRAVEWNDMCR
GASHWIEGPDEAARRIGIRPLMSGVKTEDEGLYTVQHRYWIATMKQALAAERSGA

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=407, Percent_Identity=26.5356265356265, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788103, Length=229, Percent_Identity=30.5676855895196, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 51781; Mature: 51781

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIDPDRRATPRPIDDFLVEDKARGDYRLHRSAFTDEMLFELEMKHIFEGNWIYLAHES
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
QLPNANDYYTTTIGRQPIVIARNRHGELNAFVNACTHRGAMLCRHKRGNRASYTCPFHGW
CCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCE
TFSNGGKLLKVKDPEGAGYPDCFNRDGSHDLKKVARFENYRGFLFGSLNPEVEPLAAHLG
EECCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
DAARIIDMIVDQSADGLEVLRGSSTYTYEGNWKLTAENGADGYHVSAVHWNYAATVNHRK
HHHHHHHHHHCCCCCCHHHHCCCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEEECCCC
TDAQHEDTIRAMDAGNWGRQGGGFYAFDHGHMLLWTRWANPEDRPNFDRRDEFAARCGGD
CCCCHHHHEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHCCH
VADWMIRNSRNLCLYPNVYLMDQFGSQIRVLRPLAVDRTEVTIYCIAPKGEAPDARARRI
HHHHHHHCCCCEEECCCEEEECCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCHHHHHH
RQYEDFFNVSGMATPDDLEEFRACQQGYAGRAVEWNDMCRGASHWIEGPDEAARRIGIRP
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCHHHHHHHCCCHH
LMSGVKTEDEGLYTVQHRYWIATMKQALAAERSGA
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPIDPDRRATPRPIDDFLVEDKARGDYRLHRSAFTDEMLFELEMKHIFEGNWIYLAHES
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
QLPNANDYYTTTIGRQPIVIARNRHGELNAFVNACTHRGAMLCRHKRGNRASYTCPFHGW
CCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCE
TFSNGGKLLKVKDPEGAGYPDCFNRDGSHDLKKVARFENYRGFLFGSLNPEVEPLAAHLG
EECCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
DAARIIDMIVDQSADGLEVLRGSSTYTYEGNWKLTAENGADGYHVSAVHWNYAATVNHRK
HHHHHHHHHHCCCCCCHHHHCCCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEEECCCC
TDAQHEDTIRAMDAGNWGRQGGGFYAFDHGHMLLWTRWANPEDRPNFDRRDEFAARCGGD
CCCCHHHHEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHCCH
VADWMIRNSRNLCLYPNVYLMDQFGSQIRVLRPLAVDRTEVTIYCIAPKGEAPDARARRI
HHHHHHHCCCCEEECCCEEEECCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCHHHHHH
RQYEDFFNVSGMATPDDLEEFRACQQGYAGRAVEWNDMCRGASHWIEGPDEAARRIGIRP
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCCCCHHHHHHHCCCHH
LMSGVKTEDEGLYTVQHRYWIATMKQALAAERSGA
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]