Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 126446961

GI number: 126446961

Start: 376702

End: 377616

Strand: Reverse

Name: gcvA [H]

Synonym: BMA10247_A0432

Alternate gene names: 126446961

Gene position: 377616-376702 (Counterclockwise)

Preceding gene: 126445757

Following gene: 126447274

Centisome position: 16.05

GC content: 72.68

Gene sequence:

>915_bases
ATGCCCGCCCGAAAGCCCGGCCGCCTGCCGCCGCTGAATGCGCTCCGCGCGTTCGAGGTGTCCGCGCGGCACCTGAACTT
CCGCGCGGCGGCCGACGAGATCGGCGTCACCCAGGGCGCGGTCGCGCAACAGGTCCGCCATCTGGAGGACGCGCTCGGCG
TGGCGCTGTTCGAACGCCTGCCGCGCGGCCTCGCGCTGACGGCCGACGGGCTCGCCTATTTCGCCGACGTGCGTCGTGCG
CTGAACCTGATCGGCGACGCGACGGACCGGCTCGCGAAGCGCCGCGCAAACGTCACGATCAGCACGACGCCCTCGTTCGC
GTCGCGCTGGCTGATTCCGCGGCTCGCGAGCTTCGGCGACGAGCATCGGGGCGTCGACCTCCGCGTGATCGCCGATTCGC
AGTTCGCGACCTTCCAGGGCGACGGCGTCGATCTCGCGATTCGCTACGGCAAGCCGCCGTTCGGCAAGGGGCTCGTCACG
CGCTTGCTGTTTCCGGTCGACATCTACGCGGTGTGCAGCCCCGCGCTGCTCGCCCCCCCCGGCGCGCTCGCCCGCGCGCG
CGATCTCGCCGGGCACGCGCTGCTGCACGACGCGCATGACCTGTGGCCCGAGTTTCTCGCCGCGCTGCCCGAGCGCGTCG
CGATCGATCCGGCCAAGGGGCCGCGCTTCAATCAATCGTCGCTCGCCATCGACGCGGCGATCGCCGGGCACGGCGTCGCG
CTCGCGAGCGATCAGCTCGTCGCGCGCGACATCGAGGCCGGCCGGCTGCGGCGGCTGTTCGATTTCGCGCTGCCGCTGTC
GGTCGGCTATTACATCGTCCGTCCGAGCGAGCCGCGGCGCCCCGGCGACATCGCGCAGACCGAGGCGTGGCTGATCCGCC
AGGCGCAGCTGGATGCGCGACGCGACGGCGCGTAG

Upstream 100 bases:

>100_bases
CGGCAACCCCCGCTGTCGAATCATCGTTTTTGACGTCAGGCGATAGTTTTTCTCCTGTTTATTCGATTTCCGCGATGTCC
ACGATTTGTCCCGCTTCGAC

Downstream 100 bases:

>100_bases
CGCGCGCGGGCCGCGTTCGTCGCGGGGCGTTCGGATCGGTCGAGGGCGCCGCGCCGCGCGGGCGGCGTCTCTGCCTCTGC
CTTCGCCTTCGCCTTCGTCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRA
LNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHRGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVT
RLLFPVDIYAVCSPALLAPPGALARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA

Sequences:

>Translated_304_residues
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRA
LNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHRGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVT
RLLFPVDIYAVCSPALLAPPGALARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA
>Mature_303_residues
PARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRAL
NLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHRGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTR
LLFPVDIYAVCSPALLAPPGALARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVAL
ASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=40.893470790378, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1786448, Length=306, Percent_Identity=31.6993464052288, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788706, Length=273, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=258, Percent_Identity=25.1937984496124, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786401, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081978, Length=280, Percent_Identity=26.4285714285714, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=24.4525547445255, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=241, Percent_Identity=24.896265560166, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=21.2121212121212, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32946; Mature: 32815

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERL
CCCCCCCCCCCHHHHHHHHHHHHHCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PRGLALTADGLAYFADVRRALNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGD
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCC
EHRGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTRLLFPVDIYAVCSPALLAPP
CCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCCC
GALARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHEEEEHHCCCCEE
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
RDGA
CCCC
>Mature Secondary Structure 
PARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERL
CCCCCCCCCCHHHHHHHHHHHHHCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PRGLALTADGLAYFADVRRALNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGD
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCC
EHRGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTRLLFPVDIYAVCSPALLAPP
CCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCCC
GALARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHEEEEHHCCCCEE
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
RDGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]