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The map label for this gene is gcvA [H]

Identifier: 126447274

GI number: 126447274

Start: 374190

End: 375131

Strand: Reverse

Name: gcvA [H]

Synonym: BMA10247_A0428

Alternate gene names: 126447274

Gene position: 375131-374190 (Counterclockwise)

Preceding gene: 126446961

Following gene: 126446396

Centisome position: 15.94

GC content: 70.17

Gene sequence:

>942_bases
ATGGATATCAAGCTCGATACGCCGTTGCTCGGCGCGCTGCGCTGCTTCGAAGCGGCGGGCCGGCTCGGCAGCTTCACGAA
GGCGGCCGACGCGCTGCACCTGACGCAAAGCGCGATCAGCCAGCAGATCCGCCAGCTCGAGGCGCGCCTCGGCTATCCGC
TGTTCGTGCGCCACGCGCGCGGGCTCGCGTTGACGCCGAAGGGCGGCGCGCTGCACGAAGCCACCGTGGCGGCATTCGCA
TCGCTCGGCCGCACGCTGCGGCAACTGAGCGCGCCCGACGCGCCGCTGCAGGTGAGCTGCCTGCCGTCGTTCGCGCTGCA
ATGGCTGATGCCGAGGCTGACCGAATTTCATCGGCTGCATCCGCAGACGCCGGTGCGGCTGAGGGCCGAGTTTCATGCGA
TCGATCGCGAGACGATGAGCGCGGACGACATCGATATCGCGATCCGCTGGGACCCGTTCAGCTATGGCGCGCTCCACGTC
GAACCGCTGTTCGACGAATCGCTCGTCGCGGCCGCCGCGCCCGCGTATCTCGACCGGCATCCGCCGCGCGCGACGGCGAA
GTGGGTGTCGAGCGTCGTGCCGCTGCACGATGCGTCGGCGTGGCCGGAGGCGCCGAGGTATGTCGAATGGCGGACGTGGC
TCGATGCGGTCGTGCCCGGCTATGCGAGCGCGCTGCGGGGGCCGGAGTTCAATCTGGCGAGCCTGGCGATCGCGGCGGCG
GTTGCGGGGCAAGGGGTGGTGGTGTGCCGGGAGGCGCTCGTCGACGACGAAATCGCGCGTGGCCGGCTCGTCAATGTGTT
CGCGCGCCGCGTGCCCGCGCCGGCGCAATACAAGGCGATTTGCGCGCGGCCGGAAGACGCGCGGGTGAAAGCGTTTGTCG
GCTGGTTGCGGGCGGAATCGGACAGTTACGCGGCGCGGCGGCAAACGCAGGCGCGGCCGTAA

Upstream 100 bases:

>100_bases
GCATGATCTGAAATAAAATGTGATGGACAAGCCCTCATCGTAAGGCCCCACGGGCCGTGCGAAGAAGCGATCAATTCTTG
CCCATGCATAAGCCATGCTA

Downstream 100 bases:

>100_bases
GCGCAGGCGCGGCGCGCGCCCCACGCGCGCGCGACTTGGGCCGCCGCCGTTCGATAGACAAGCATTGGATGGCGGATCGA
ACGCCGCGAAAAATCAGGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYPLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGALHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYASALRGPEFNLASLAIAAA
VAGQGVVVCREALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP

Sequences:

>Translated_313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYPLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGALHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYASALRGPEFNLASLAIAAA
VAGQGVVVCREALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP
>Mature_313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYPLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGALHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYASALRGPEFNLASLAIAAA
VAGQGVVVCREALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=35.2348993288591, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.9319727891156, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786448, Length=261, Percent_Identity=35.2490421455939, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=24.6575342465753, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790262, Length=233, Percent_Identity=27.0386266094421, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=24.5098039215686, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788887, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=275, Percent_Identity=25.4545454545455, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34350; Mature: 34350

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYPLFVRHAR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GLALTPKGGALHEATVAAFASLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLH
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
PQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGALHVEPLFDESLVAAAAPAYLDRH
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCC
PPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYASALRGPEFNLASLAIAAA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VAGQGVVVCREALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAES
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHCCC
DSYAARRQTQARP
CHHHHHHHHCCCC
>Mature Secondary Structure
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYPLFVRHAR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GLALTPKGGALHEATVAAFASLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLH
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
PQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGALHVEPLFDESLVAAAAPAYLDRH
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCC
PPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYASALRGPEFNLASLAIAAA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VAGQGVVVCREALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAES
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHCCC
DSYAARRQTQARP
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]