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The map label for this gene is bkdB [H]

Identifier: 126446955

GI number: 126446955

Start: 2229025

End: 2230476

Strand: Reverse

Name: bkdB [H]

Synonym: BMA10247_A2300

Alternate gene names: 126446955

Gene position: 2230476-2229025 (Counterclockwise)

Preceding gene: 126446124

Following gene: 126447169

Centisome position: 94.81

GC content: 72.18

Gene sequence:

>1452_bases
ATGGGCGTGCACGTTATCAAGATGCCGGACATCGGCGAAGGGATCGCGGAGGTCGAGCTCGGGCTGTGGCACGTGAAGGT
CGGCGATCGCGTGAAGGAAGACCAGGCGACCGCCGACGTGATGACCGACAAGGCGTCGGTCGAGATTCCGTCGCCCGTCA
CGGGCGTCGTCGTCGCGTTGGGCGGCAAGGAAGGCGACGTGCTCGCGGTGGGCAGCGAGCTCGTGCGGCTCGAAGTCGAA
GGCGACGGCAATCACAAGGCCGAGCCCGACGGCGGCGCGCGCGCCGCCGCCGCGCAGCCCGAACGCGTCGCCGACACGGC
GCACGCGCATGCGAGCGCGGCTGCGAAATCCGCGCGCGGCGAGCACGGTGCCGGCCACGGGCGCGACGATGCGCGCGCCG
CATCGAGCGGCACATCGAGCGGCGCTTCGCACACGCAGCACGCCGAACGTGAGGCGCGCGGGCATCGCGAATCCAGCGAA
TACCGCGAAGACCGAAGCGTGTCGCGCGGCGCTTCGCAAACCGACGTCGAACGCGGGCCCGCGTCGCCGCCGCCCGCGCG
CCGGCCGGGCGAGCGCCCGCTCGCGTCGCCCGCGGTGCGCAAGCGCGCGTGGGATCTCGGCGTCGAGCTGCGCTACGTGC
ACGGCACGGGCGAGGCAGGCCGAATCCTGCACGAGGATCTCGACGCGTACCTGCAAGGCCGCGGCGCGGCCGCGCAGCGC
GCGCGCGGCGGCCAGGCCGCGTACGTCGAGCGCCACGACGAAGAGGCGGTGCCCGTGATCGGCCTGCGCCGCAAGATCGC
GCAGCGGATGCAGGACGCGAAGCGGCGCATCCCGCACTTCAGCTACGTCGAGGAGATCGACGTCACCGAGCTCGAAGCGC
TGCGCGCGGAGCTGAACCGCAAGTATGGCGACACGCGCGGCCGGCTGACGGTGTTGCCGCTGCTCGCGCGCGCGATGGTG
ATCGCGCTGCGCGAGTTCCCGCAGATCAACGCGCGCTACGACGACGAAGCCGGCGTCGTCACGCGTCACGGCGCGGTGCA
TCTGGGCATCGCGACGCAGAGCAAGGCGGGCCTGATGGTGCCCGTCGTGCGCCATGCGGAGGCGCGCGATCCGTGGTCGA
TCGCGGCCGAGGTCGCGCGGCTCGCGGATGCGGCGCGCGCGGGCCGCGCGGAGCGCGACGAGCTGTCGGGCTCGACGATC
ACGATCACGAGCCTGGGCGCGCTGGGCGGCATCGCGTCGACGCCCGTCATCAATTCGCCCGAAGTCGGCATCGTCGGCGT
GAACCGGATCGTCGAGCGGCCGATGTTCCGCGGCGGCGCGGTGGTCGCGCGCAAGCTGATGAACCTGTCTTCGTCGTTCG
ATCACCGCGTGATCGACGGCATGGACGCGGCCGAGTTCATCCAGGCCGTGCGCGCGCTGCTCGAGCAGCCCGCCCTTCTT
TTCGTGGAATGA

Upstream 100 bases:

>100_bases
ACCGGCTGGGACACGCCGTATCCGCACGCGCAGGAATGGGCGTATTTCCCGGGCCCGAACCGGGTCGGCGACGCGCTGCG
GCGCGCGATGGAGAATTGAC

Downstream 100 bases:

>100_bases
GCCGATGAGCGACACCAAGACAACGACCCTGCTCGTGATCGGCGGCGGGCCGGGCGGCTATGTCGCCGCGATTCGGGCGG
GGCAGCTCGGCGTGCGCACG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGVHVIKMPDIGEGIAEVELGLWHVKVGDRVKEDQATADVMTDKASVEIPSPVTGVVVALGGKEGDVLAVGSELVRLEVE
GDGNHKAEPDGGARAAAAQPERVADTAHAHASAAAKSARGEHGAGHGRDDARAASSGTSSGASHTQHAEREARGHRESSE
YREDRSVSRGASQTDVERGPASPPPARRPGERPLASPAVRKRAWDLGVELRYVHGTGEAGRILHEDLDAYLQGRGAAAQR
ARGGQAAYVERHDEEAVPVIGLRRKIAQRMQDAKRRIPHFSYVEEIDVTELEALRAELNRKYGDTRGRLTVLPLLARAMV
IALREFPQINARYDDEAGVVTRHGAVHLGIATQSKAGLMVPVVRHAEARDPWSIAAEVARLADAARAGRAERDELSGSTI
TITSLGALGGIASTPVINSPEVGIVGVNRIVERPMFRGGAVVARKLMNLSSSFDHRVIDGMDAAEFIQAVRALLEQPALL
FVE

Sequences:

>Translated_483_residues
MGVHVIKMPDIGEGIAEVELGLWHVKVGDRVKEDQATADVMTDKASVEIPSPVTGVVVALGGKEGDVLAVGSELVRLEVE
GDGNHKAEPDGGARAAAAQPERVADTAHAHASAAAKSARGEHGAGHGRDDARAASSGTSSGASHTQHAEREARGHRESSE
YREDRSVSRGASQTDVERGPASPPPARRPGERPLASPAVRKRAWDLGVELRYVHGTGEAGRILHEDLDAYLQGRGAAAQR
ARGGQAAYVERHDEEAVPVIGLRRKIAQRMQDAKRRIPHFSYVEEIDVTELEALRAELNRKYGDTRGRLTVLPLLARAMV
IALREFPQINARYDDEAGVVTRHGAVHLGIATQSKAGLMVPVVRHAEARDPWSIAAEVARLADAARAGRAERDELSGSTI
TITSLGALGGIASTPVINSPEVGIVGVNRIVERPMFRGGAVVARKLMNLSSSFDHRVIDGMDAAEFIQAVRALLEQPALL
FVE
>Mature_482_residues
GVHVIKMPDIGEGIAEVELGLWHVKVGDRVKEDQATADVMTDKASVEIPSPVTGVVVALGGKEGDVLAVGSELVRLEVEG
DGNHKAEPDGGARAAAAQPERVADTAHAHASAAAKSARGEHGAGHGRDDARAASSGTSSGASHTQHAEREARGHRESSEY
REDRSVSRGASQTDVERGPASPPPARRPGERPLASPAVRKRAWDLGVELRYVHGTGEAGRILHEDLDAYLQGRGAAAQRA
RGGQAAYVERHDEEAVPVIGLRRKIAQRMQDAKRRIPHFSYVEEIDVTELEALRAELNRKYGDTRGRLTVLPLLARAMVI
ALREFPQINARYDDEAGVVTRHGAVHLGIATQSKAGLMVPVVRHAEARDPWSIAAEVARLADAARAGRAERDELSGSTIT
ITSLGALGGIASTPVINSPEVGIVGVNRIVERPMFRGGAVVARKLMNLSSSFDHRVIDGMDAAEFIQAVRALLEQPALLF
VE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=315, Percent_Identity=32.0634920634921, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI31711992, Length=313, Percent_Identity=30.6709265175719, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=30.4932735426009, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI203098816, Length=231, Percent_Identity=26.8398268398268, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI203098753, Length=231, Percent_Identity=26.8398268398268, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=28.75, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786305, Length=294, Percent_Identity=30.952380952381, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1786946, Length=292, Percent_Identity=29.4520547945205, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=317, Percent_Identity=30.2839116719243, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=232, Percent_Identity=31.8965517241379, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=26.2921348314607, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=25.08038585209, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=25.7777777777778, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=220, Percent_Identity=30.4545454545455, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=301, Percent_Identity=28.9036544850498, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=32.4675324675325, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=29.6943231441048, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=29.6943231441048, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 51543; Mature: 51411

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVHVIKMPDIGEGIAEVELGLWHVKVGDRVKEDQATADVMTDKASVEIPSPVTGVVVAL
CCEEEEECCCCCCCHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCCEEEEEE
GGKEGDVLAVGSELVRLEVEGDGNHKAEPDGGARAAAAQPERVADTAHAHASAAAKSARG
CCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
EHGAGHGRDDARAASSGTSSGASHTQHAEREARGHRESSEYREDRSVSRGASQTDVERGP
CCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCC
ASPPPARRPGERPLASPAVRKRAWDLGVELRYVHGTGEAGRILHEDLDAYLQGRGAAAQR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHH
ARGGQAAYVERHDEEAVPVIGLRRKIAQRMQDAKRRIPHFSYVEEIDVTELEALRAELNR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
KYGDTRGRLTVLPLLARAMVIALREFPQINARYDDEAGVVTRHGAVHLGIATQSKAGLMV
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCEE
PVVRHAEARDPWSIAAEVARLADAARAGRAERDELSGSTITITSLGALGGIASTPVINSP
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECHHHCCCCCCCCCCCC
EVGIVGVNRIVERPMFRGGAVVARKLMNLSSSFDHRVIDGMDAAEFIQAVRALLEQPALL
CCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCEE
FVE
EEC
>Mature Secondary Structure 
GVHVIKMPDIGEGIAEVELGLWHVKVGDRVKEDQATADVMTDKASVEIPSPVTGVVVAL
CEEEEECCCCCCCHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCCEEEEEE
GGKEGDVLAVGSELVRLEVEGDGNHKAEPDGGARAAAAQPERVADTAHAHASAAAKSARG
CCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
EHGAGHGRDDARAASSGTSSGASHTQHAEREARGHRESSEYREDRSVSRGASQTDVERGP
CCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCC
ASPPPARRPGERPLASPAVRKRAWDLGVELRYVHGTGEAGRILHEDLDAYLQGRGAAAQR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHH
ARGGQAAYVERHDEEAVPVIGLRRKIAQRMQDAKRRIPHFSYVEEIDVTELEALRAELNR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
KYGDTRGRLTVLPLLARAMVIALREFPQINARYDDEAGVVTRHGAVHLGIATQSKAGLMV
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCEE
PVVRHAEARDPWSIAAEVARLADAARAGRAERDELSGSTITITSLGALGGIASTPVINSP
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECHHHCCCCCCCCCCCC
EVGIVGVNRIVERPMFRGGAVVARKLMNLSSSFDHRVIDGMDAAEFIQAVRALLEQPALL
CCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCEE
FVE
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]