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The map label for this gene is lpdV [H]

Identifier: 126447169

GI number: 126447169

Start: 2227620

End: 2229020

Strand: Reverse

Name: lpdV [H]

Synonym: BMA10247_A2299

Alternate gene names: 126447169

Gene position: 2229020-2227620 (Counterclockwise)

Preceding gene: 126446955

Following gene: 126447195

Centisome position: 94.74

GC content: 71.16

Gene sequence:

>1401_bases
ATGAGCGACACCAAGACAACGACCCTGCTCGTGATCGGCGGCGGGCCGGGCGGCTATGTCGCCGCGATTCGGGCGGGGCA
GCTCGGCGTGCGCACGATTCTCGTCGAGCGCGACAGGCTGGGCGGCACGTGCCTGAACATCGGCTGCATTCCGTCGAAGG
CGCTCATTCACGCGGCGGGCGAATTCGACAAGGTGCGCGGCTTCGCCGGCGATTCGCCGCTCGGCATCCGCACGCAGGCG
CCCGCGATCGACATCGCGCGCACGGTGGCGTGGAAGGACGGCATCGTGAAGAAGCTGACGGGCGGCGTCGGCGCGCTCCT
GAAGAAGAACGGCGTCGAAGTCGTGCACGGCGACGCGCGCGTCGTCGACGGCAAGACCGTCGACGTCGACACGGGCGGCG
GCGCGCGCGTGCGGATCCAGTGCGAGCATCTGCTGCTCGCCGCGGGCTCCGAGCCCGTCGAGCTGCCGGCGATGCCGTTC
GGCGGCAACGTGATCTCGTCGACCGGCGCGTTGTCGCCGGGCAGGCTGCCGAAGCGGCTCGTCGTCGTCGGGGCGGGCTA
CATCGGGCTCGAGCTCGGCATCGCGTACCGCAAGCTCGGCGTCGAGGTGAGCGTCGTCGAGGCGCGCGAGCGCATCCTGC
CGATCTACGACGCGGAGCTGACGAAGCCCGTGGCCGCCTCGCTCAAGCGCCTCGGCGTGCGCGTGCTGCTCGGCCACAAG
GTGCTCGGGCTGAACGCGCGCGGCGATGCGGTGTGCGTGCAGGACGACGCGCACGCGCAAACCGAGCTCGCCGCCGATCA
GGTGCTCGTGACGGTCGGCCGCCGTCCGCGCACGCAGGGCTGGGGGCTCGAGACGCTGCAGCTCGACCGCGCGGGCGCCG
CGCTGAAAGTCGACGACATGTGCCGCACGTCGATGCGCAACGTCTGGGCGATCGGCGATCTGACGGGCGAGCCGATGCTC
GCGCATCGCGCGATGGCGCAAGGCGAGATGGTCGCCGAGATCGTGGCCGGCAAGAAGCGCCATTTCATGCCCGCCGCGAT
CGCCGCGATCTGCTTCACCGATCCCGAGGTCGTCTCGGCCGGGCTCGCGCCCGACGAAGCCGAGCGCACGTTCGGCGCGT
GCGTGAGCGCGTCGTTTCCGTTCGCCGCGAACGGCCGCGCGCTGACGCTCGAGAGCGCGGACGGCTTCGTGCGCGTCGTC
GCGCGCCGCGACGATCACCTGATCGTCGGCTGGCAGGCGGTGGGCGCCGGCGTGTCCGAACTCGCGGCCGCGTTCTCGCA
ATCGCTCGAAATGGGTGCGCGCCTCGAGGACGTCGGTGGTACGATTCATGCGCACCCGACGCTCGGAGAAGCGGTGATGG
AAGCCGCGCTTCGCGCGCTCGGACACGCGTTGCACATCTGA

Upstream 100 bases:

>100_bases
TTCGATCACCGCGTGATCGACGGCATGGACGCGGCCGAGTTCATCCAGGCCGTGCGCGCGCTGCTCGAGCAGCCCGCCCT
TCTTTTCGTGGAATGAGCCG

Downstream 100 bases:

>100_bases
AGCACGCATTCGCGATCACGCGCGCCGCGCGGATCGCGTCCGACATCAACGACGCCGGGACGCGTCGCGATCCCGGCCAT
CAGGCACAGAGGCGAGCGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDTKTTTLLVIGGGPGGYVAAIRAGQLGVRTILVERDRLGGTCLNIGCIPSKALIHAAGEFDKVRGFAGDSPLGIRTQA
PAIDIARTVAWKDGIVKKLTGGVGALLKKNGVEVVHGDARVVDGKTVDVDTGGGARVRIQCEHLLLAAGSEPVELPAMPF
GGNVISSTGALSPGRLPKRLVVVGAGYIGLELGIAYRKLGVEVSVVEARERILPIYDAELTKPVAASLKRLGVRVLLGHK
VLGLNARGDAVCVQDDAHAQTELAADQVLVTVGRRPRTQGWGLETLQLDRAGAALKVDDMCRTSMRNVWAIGDLTGEPML
AHRAMAQGEMVAEIVAGKKRHFMPAAIAAICFTDPEVVSAGLAPDEAERTFGACVSASFPFAANGRALTLESADGFVRVV
ARRDDHLIVGWQAVGAGVSELAAAFSQSLEMGARLEDVGGTIHAHPTLGEAVMEAALRALGHALHI

Sequences:

>Translated_466_residues
MSDTKTTTLLVIGGGPGGYVAAIRAGQLGVRTILVERDRLGGTCLNIGCIPSKALIHAAGEFDKVRGFAGDSPLGIRTQA
PAIDIARTVAWKDGIVKKLTGGVGALLKKNGVEVVHGDARVVDGKTVDVDTGGGARVRIQCEHLLLAAGSEPVELPAMPF
GGNVISSTGALSPGRLPKRLVVVGAGYIGLELGIAYRKLGVEVSVVEARERILPIYDAELTKPVAASLKRLGVRVLLGHK
VLGLNARGDAVCVQDDAHAQTELAADQVLVTVGRRPRTQGWGLETLQLDRAGAALKVDDMCRTSMRNVWAIGDLTGEPML
AHRAMAQGEMVAEIVAGKKRHFMPAAIAAICFTDPEVVSAGLAPDEAERTFGACVSASFPFAANGRALTLESADGFVRVV
ARRDDHLIVGWQAVGAGVSELAAAFSQSLEMGARLEDVGGTIHAHPTLGEAVMEAALRALGHALHI
>Mature_465_residues
SDTKTTTLLVIGGGPGGYVAAIRAGQLGVRTILVERDRLGGTCLNIGCIPSKALIHAAGEFDKVRGFAGDSPLGIRTQAP
AIDIARTVAWKDGIVKKLTGGVGALLKKNGVEVVHGDARVVDGKTVDVDTGGGARVRIQCEHLLLAAGSEPVELPAMPFG
GNVISSTGALSPGRLPKRLVVVGAGYIGLELGIAYRKLGVEVSVVEARERILPIYDAELTKPVAASLKRLGVRVLLGHKV
LGLNARGDAVCVQDDAHAQTELAADQVLVTVGRRPRTQGWGLETLQLDRAGAALKVDDMCRTSMRNVWAIGDLTGEPMLA
HRAMAQGEMVAEIVAGKKRHFMPAAIAAICFTDPEVVSAGLAPDEAERTFGACVSASFPFAANGRALTLESADGFVRVVA
RRDDHLIVGWQAVGAGVSELAAAFSQSLEMGARLEDVGGTIHAHPTLGEAVMEAALRALGHALHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=36.304347826087, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=26.9978401727862, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI22035672, Length=468, Percent_Identity=26.7094017094017, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045266, Length=436, Percent_Identity=23.1651376146789, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI148277065, Length=442, Percent_Identity=22.6244343891403, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519430, Length=442, Percent_Identity=22.6244343891403, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519428, Length=442, Percent_Identity=22.6244343891403, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519426, Length=442, Percent_Identity=22.6244343891403, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI148277071, Length=442, Percent_Identity=22.6244343891403, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=22.7699530516432, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=37.3650107991361, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1789915, Length=441, Percent_Identity=27.6643990929705, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=26.963906581741, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=24.6724890829694, Blast_Score=125, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=39.3162393162393, Blast_Score=298, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=27.3755656108597, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=27.3755656108597, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=23.6673773987207, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=36.4779874213836, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.1762208067941, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=25.6842105263158, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=38.3795309168444, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=26.7223382045929, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=26.9311064718163, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24640551, Length=479, Percent_Identity=26.9311064718163, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=26.4392324093817, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48576; Mature: 48445

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTKTTTLLVIGGGPGGYVAAIRAGQLGVRTILVERDRLGGTCLNIGCIPSKALIHAAG
CCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCHHHHEECC
EFDKVRGFAGDSPLGIRTQAPAIDIARTVAWKDGIVKKLTGGVGALLKKNGVEVVHGDAR
CHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCEEEECCEE
VVDGKTVDVDTGGGARVRIQCEHLLLAAGSEPVELPAMPFGGNVISSTGALSPGRLPKRL
EECCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEE
VVVGAGYIGLELGIAYRKLGVEVSVVEARERILPIYDAELTKPVAASLKRLGVRVLLGHK
EEEECCCEEEHHHHHHHHHCCEEEEEEHHHHCCCEECCCCCHHHHHHHHHCCEEEEECCE
VLGLNARGDAVCVQDDAHAQTELAADQVLVTVGRRPRTQGWGLETLQLDRAGAALKVDDM
EEEECCCCCEEEEECCCCHHHHHHHHHEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHH
CRTSMRNVWAIGDLTGEPMLAHRAMAQGEMVAEIVAGKKRHFMPAAIAAICFTDPEVVSA
HHHHHCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHCC
GLAPDEAERTFGACVSASFPFAANGRALTLESADGFVRVVARRDDHLIVGWQAVGAGVSE
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCEEEEEHHHHCCCHHH
LAAAFSQSLEMGARLEDVGGTIHAHPTLGEAVMEAALRALGHALHI
HHHHHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTKTTTLLVIGGGPGGYVAAIRAGQLGVRTILVERDRLGGTCLNIGCIPSKALIHAAG
CCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCHHHHEECC
EFDKVRGFAGDSPLGIRTQAPAIDIARTVAWKDGIVKKLTGGVGALLKKNGVEVVHGDAR
CHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCEEEECCEE
VVDGKTVDVDTGGGARVRIQCEHLLLAAGSEPVELPAMPFGGNVISSTGALSPGRLPKRL
EECCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEE
VVVGAGYIGLELGIAYRKLGVEVSVVEARERILPIYDAELTKPVAASLKRLGVRVLLGHK
EEEECCCEEEHHHHHHHHHCCEEEEEEHHHHCCCEECCCCCHHHHHHHHHCCEEEEECCE
VLGLNARGDAVCVQDDAHAQTELAADQVLVTVGRRPRTQGWGLETLQLDRAGAALKVDDM
EEEECCCCCEEEEECCCCHHHHHHHHHEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHH
CRTSMRNVWAIGDLTGEPMLAHRAMAQGEMVAEIVAGKKRHFMPAAIAAICFTDPEVVSA
HHHHHCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHCC
GLAPDEAERTFGACVSASFPFAANGRALTLESADGFVRVVARRDDHLIVGWQAVGAGVSE
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCEEEEEHHHHCCCHHH
LAAAFSQSLEMGARLEDVGGTIHAHPTLGEAVMEAALRALGHALHI
HHHHHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]