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The map label for this gene is pksR [H]

Identifier: 126446905

GI number: 126446905

Start: 1254693

End: 1255556

Strand: Reverse

Name: pksR [H]

Synonym: BMA10247_A1291

Alternate gene names: 126446905

Gene position: 1255556-1254693 (Counterclockwise)

Preceding gene: 126446689

Following gene: 126446343

Centisome position: 53.37

GC content: 65.51

Gene sequence:

>864_bases
ATGCGCCACCTTCGCGCGGATGAACTCGAAATCCACCGCCGCATCGATCTGGCGCAGCAGGTGGTCCTTCGGCACGAGTT
CCTCGAGCGTCACCATCTCGAGTTCGTGCTGCGTGGGCATGGGCGTCTTCAGCATCACGCCATTAAAAAACAAAAATCCC
CCCCACTTGGCGAGGGTTTGTCAGCAATCTGGGCGCACTACGTGCGCTGCATCCGCAGCGTGCAGCCCGCGGGGCCGTAC
TTCCTCGGCGGGCATTCGCTCGGCGGCAAGATCGCCTATGAAGTCGCACGCCGGCTGCATGCGCAGGGCGACGCGATCGG
CCTCGTCGCGATGTTCGATTCCGCCGCGCCGCCGTATTCGTTCGTCGCGCATCAGGACGATTTCGCGATCGCCAGCATGA
TTCTCGGCGTCTTCGCGTACTACGCGGGCAAGATGGAGATGATGGCCGGCATCGACGACGCGCGCTTGCGCGACGCGCCG
CGCGAGCGGTTGCTCGCGTTCATGGGCGAGCGGCTCGCGCAGTTCGGCGTGATTCAGTCGCAAAGCGATACGAGCGCGAT
TCGCGGGCTCTTCAACGTGTATCGCGCGGCGGCCGATTTTTCCGCGCGCTATGCGCCGCCGCACGAGCACCTGCCGCTGC
CGATACTGCTCGTCAAGGCGACCGAGCCGATGCCCGACGGCATCAAGCTGCCCGAAATCCGCGAGACGCCGGCGTGGGGC
TGGGAAAACTTCACGCGGCTGCCGGTGCGCACGTGCGAAGTCGCGGGCAACCATTACAGTTGCCTGATGGACGGCTACGT
CGAGCGCATCGCCGATGCGCTGCGCGATGCGCTGGCGTCGGCGCGGCAAGCGATCGAGGCGTGA

Upstream 100 bases:

>100_bases
GTTGCCGCTCGCTGCGCACCCCGAACAGGTAGCCGATGAACAACAGCTTGAACATCACCACGGGATCGAGCGCCGGCCGC
CCGTTGTCCGCGCAATACAG

Downstream 100 bases:

>100_bases
CGCGATGGATCTCCACGAACGCACGCTCGACGCGCCGGGCGCACCGGCGGCCGCGGCGCACGGCGCCGGCTCGCGTCTGT
CGCGCGAGGTCTGGATCATT

Product: putative non-ribosomal peptide synthase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRHLRADELEIHRRIDLAQQVVLRHEFLERHHLEFVLRGHGRLQHHAIKKQKSPPLGEGLSAIWAHYVRCIRSVQPAGPY
FLGGHSLGGKIAYEVARRLHAQGDAIGLVAMFDSAAPPYSFVAHQDDFAIASMILGVFAYYAGKMEMMAGIDDARLRDAP
RERLLAFMGERLAQFGVIQSQSDTSAIRGLFNVYRAAADFSARYAPPHEHLPLPILLVKATEPMPDGIKLPEIRETPAWG
WENFTRLPVRTCEVAGNHYSCLMDGYVERIADALRDALASARQAIEA

Sequences:

>Translated_287_residues
MRHLRADELEIHRRIDLAQQVVLRHEFLERHHLEFVLRGHGRLQHHAIKKQKSPPLGEGLSAIWAHYVRCIRSVQPAGPY
FLGGHSLGGKIAYEVARRLHAQGDAIGLVAMFDSAAPPYSFVAHQDDFAIASMILGVFAYYAGKMEMMAGIDDARLRDAP
RERLLAFMGERLAQFGVIQSQSDTSAIRGLFNVYRAAADFSARYAPPHEHLPLPILLVKATEPMPDGIKLPEIRETPAWG
WENFTRLPVRTCEVAGNHYSCLMDGYVERIADALRDALASARQAIEA
>Mature_287_residues
MRHLRADELEIHRRIDLAQQVVLRHEFLERHHLEFVLRGHGRLQHHAIKKQKSPPLGEGLSAIWAHYVRCIRSVQPAGPY
FLGGHSLGGKIAYEVARRLHAQGDAIGLVAMFDSAAPPYSFVAHQDDFAIASMILGVFAYYAGKMEMMAGIDDARLRDAP
RERLLAFMGERLAQFGVIQSQSDTSAIRGLFNVYRAAADFSARYAPPHEHLPLPILLVKATEPMPDGIKLPEIRETPAWG
WENFTRLPVRTCEVAGNHYSCLMDGYVERIADALRDALASARQAIEA

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism [H]

COG id: COG3319

COG function: function code Q; Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR013217
- InterPro:   IPR006163
- InterPro:   IPR020841
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR001031
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08242 Methyltransf_12; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 32136; Mature: 32136

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLRADELEIHRRIDLAQQVVLRHEFLERHHLEFVLRGHGRLQHHAIKKQKSPPLGEGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCH
SAIWAHYVRCIRSVQPAGPYFLGGHSLGGKIAYEVARRLHAQGDAIGLVAMFDSAAPPYS
HHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
FVAHQDDFAIASMILGVFAYYAGKMEMMAGIDDARLRDAPRERLLAFMGERLAQFGVIQS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QSDTSAIRGLFNVYRAAADFSARYAPPHEHLPLPILLVKATEPMPDGIKLPEIRETPAWG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
WENFTRLPVRTCEVAGNHYSCLMDGYVERIADALRDALASARQAIEA
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHLRADELEIHRRIDLAQQVVLRHEFLERHHLEFVLRGHGRLQHHAIKKQKSPPLGEGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCH
SAIWAHYVRCIRSVQPAGPYFLGGHSLGGKIAYEVARRLHAQGDAIGLVAMFDSAAPPYS
HHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
FVAHQDDFAIASMILGVFAYYAGKMEMMAGIDDARLRDAPRERLLAFMGERLAQFGVIQS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QSDTSAIRGLFNVYRAAADFSARYAPPHEHLPLPILLVKATEPMPDGIKLPEIRETPAWG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
WENFTRLPVRTCEVAGNHYSCLMDGYVERIADALRDALASARQAIEA
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]