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The map label for this gene is leuB [H]

Identifier: 126446612

GI number: 126446612

Start: 469136

End: 470203

Strand: Direct

Name: leuB [H]

Synonym: BMA10247_A0523

Alternate gene names: 126446612

Gene position: 469136-470203 (Clockwise)

Preceding gene: 126446106

Following gene: 126447206

Centisome position: 19.94

GC content: 67.7

Gene sequence:

>1068_bases
ATGAAGATTGCAGTGCTGCCCGGCGACGGCATCGGTCCGGAAATCGTCAATGAAGCGGTGAAGGTGCTGAACGCGCTCGA
CGAAAAGTTCGAACTGGAGCAGGCGCCGGTCGGCGGCGCCGGCTACGAGGCAAGCGGCCATCCGCTGCCCGACGCGACGC
TCGCGCTCGCGAAGGAAGCGGACGCGATCCTGTTCGGCGCGGTCGGCGACTGGAAGTACGATTCGCTCGAGCGCGCGCTG
CGCCCCGAGCAGGCGATCCTCGGCCTGCGCAAGCATCTGGAGCTGTTCGCGAACTTCCGTCCGGCGATCTGCTATCCGCA
GCTCGTCGACGCTTCGCCGCTCAAGCCCGAGCTCGTCGCGGGCCTCGACATCCTGATCGTGCGCGAACTGAACGGCGATA
TCTACTTCGGCCAGCCGCGCGGCGTGCGCGCCGCGCCGGACGGCCCGTTCGCGGGCGCGCGCGAAGGCTTCGACACGATG
CGCTATTCGGAGCCGGAAGTGCGCCGCATCGCGCACGTCGCGTTCCAGGCCGCGCGAAAGCGCGCGAAGAAGCTGCTGTC
GGTCGACAAATCGAACGTGCTCGAGACGTCGCAGTTCTGGCGCGACGTGATGATCGACGTGTCGAAGGAATACGCGGACG
TCGAGCTGTCACACATGTACGTCGACAACGCGGCGATGCAGCTCGCGAAGGCGCCGAAGCAGTTCGACGTGATCGTGACG
GGCAACATGTTCGGCGACATTTTGTCCGACGAGGCGTCGATGCTGACGGGCTCGATCGGCATGCTGCCGTCCGCGTCGCT
CGACCAGCGCAACAAGGGCCTGTACGAGCCGTCGCACGGCTCCGCGCCGGACATCGCGGGCAAGGGCATCGCGAATCCGC
TCGCGACGATCCTATCGGCCGCGATGCTGCTGCGCTACTCGCTGAACCGCGCGGAGCAGGCCGACCGCATCGAGCGCGCG
GTCAAGGCGGTGCTCGAGCAGGGCTACCGCACGGGCGACATCGCGACGCCGGGCTGCAAGCAGGTGGGCACGGCCGCGAT
GGGCGACGCGGTGGTCGCGGCGCTGTAA

Upstream 100 bases:

>100_bases
GGCCGAGCGGCTCGCGAAGCAGCCGTGGCTCGACAACCGGCTGGTCGGCTGACGCGTCAGGTCGCCCGACTCCCCTCATT
CATCTAGTCAGGAATTTCGC

Downstream 100 bases:

>100_bases
GCGCCGCGGGCCCGGCGCGACGGCCCGCGCGTCGTGCGCGGTGCGTCGCCGGGAAACGGCCCGGCGGCCCCGAAAACGGG
GTCCGTCCGGCTTTCCCGGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEQAPVGGAGYEASGHPLPDATLALAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAARKRAKKLLSVDKSNVLETSQFWRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL

Sequences:

>Translated_355_residues
MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEQAPVGGAGYEASGHPLPDATLALAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAARKRAKKLLSVDKSNVLETSQFWRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
>Mature_355_residues
MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEQAPVGGAGYEASGHPLPDATLALAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAARKRAKKLLSVDKSNVLETSQFWRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.0030395136778, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI28178821, Length=307, Percent_Identity=26.3843648208469, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4758582, Length=340, Percent_Identity=27.0588235294118, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178816, Length=307, Percent_Identity=26.3843648208469, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178838, Length=328, Percent_Identity=26.8292682926829, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=53.2394366197183, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=347, Percent_Identity=34.2939481268012, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=25.8793969849246, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=347, Percent_Identity=29.1066282420749, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=29.9703264094956, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=338, Percent_Identity=30.4733727810651, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=340, Percent_Identity=29.4117647058824, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=45.4794520547945, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=37.2159090909091, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=367, Percent_Identity=30.2452316076294, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=28.2282282282282, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24643270, Length=345, Percent_Identity=31.8840579710145, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643268, Length=345, Percent_Identity=31.8840579710145, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24661184, Length=347, Percent_Identity=29.6829971181556, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=26.7241379310345, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161078637, Length=365, Percent_Identity=26.5753424657534, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078633, Length=365, Percent_Identity=26.5753424657534, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=365, Percent_Identity=26.5753424657534, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=363, Percent_Identity=26.7217630853994, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281362242, Length=363, Percent_Identity=27.8236914600551, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648872, Length=363, Percent_Identity=27.8236914600551, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=29.6511627906977, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38187; Mature: 38187

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEQAPVGGAGYEASGHPLPDATLALAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGVRAAPDGPFAGAREGFDTMRYSEPEVRRIAHVAFQAARK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCHHHHHHHHHHHHHHHH
RAKKLLSVDKSNVLETSQFWRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMLLRYSLNRAEQADRIERAVKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEQAPVGGAGYEASGHPLPDATLALAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGVRAAPDGPFAGAREGFDTMRYSEPEVRRIAHVAFQAARK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCHHHHHHHHHHHHHHHH
RAKKLLSVDKSNVLETSQFWRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLDQRNKGLYEPSHGSAPDIAGKGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMLLRYSLNRAEQADRIERAVKAVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]