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The map label for this gene is leuD

Identifier: 126446106

GI number: 126446106

Start: 468437

End: 469087

Strand: Direct

Name: leuD

Synonym: BMA10247_A0522

Alternate gene names: 126446106

Gene position: 468437-469087 (Clockwise)

Preceding gene: 126447682

Following gene: 126446612

Centisome position: 19.91

GC content: 63.59

Gene sequence:

>651_bases
ATGGAAAAATTCAATGTGCATACCGGCGTCGTGGCGCCGCTCGATCGCGAGAACGTCGACACCGACGCGATCATTCCGAA
GCAGTTCCTGAAGTCGATCAAGCGCACGGGCTTCGGCCCGAACGCGTTCGACGAGTGGCGCTATCTCGATCACGGCGAGC
CCGGCCAGGACAACTCGAAGCGTCCGCTGAACCCGGACTTCGTGCTGAACCAGCCGCGCTATCAGGGCGCATCGGTGCTG
CTCGCGCGCAAGAACTTCGGCTGCGGCAGCTCGCGCGAGCACGCGCCGTGGGCGCTGCAGCAGTACGGCTTTCGCGCGAT
CGTCGCGCCGAGCTTCGCCGACATTTTCTTCAACAACTGCTACAAGAACGGCCTGCTGCCGATCGTGCTGACCGAGCAGC
AGGTCGACCACCTGTTCAACGACACGTACGCGTTCAACGGCTATCAGCTGACGATCGATCTGGACGCGCAGGTCGTGCGC
GCGCCGGACGGCCGCGAGTATCCGTTTGAAATCACCGCGTTTCGCAAGTACTGCCTGCTGAACGGCTTCGACGACATCGG
GCTCACGCTGCGGCACGCGGACAAGATCCGTCAGTTCGAGGCCGAGCGGCTCGCGAAGCAGCCGTGGCTCGACAACCGGC
TGGTCGGCTGA

Upstream 100 bases:

>100_bases
CGATCAAGCGCGCGGCGGACTGACGGCGCGCGCGAGCGCGCCGATTCGAGCTTCGATTTTGCCGGCCCGTGCGGCCGGCC
TCCAATCTGGTTGACGGATC

Downstream 100 bases:

>100_bases
CGCGTCAGGTCGCCCGACTCCCCTCATTCATCTAGTCAGGAATTTCGCATGAAGATTGCAGTGCTGCCCGGCGACGGCAT
CGGTCCGGAAATCGTCAATG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MEKFNVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIVAPSFADIFFNNCYKNGLLPIVLTEQQVDHLFNDTYAFNGYQLTIDLDAQVVR
APDGREYPFEITAFRKYCLLNGFDDIGLTLRHADKIRQFEAERLAKQPWLDNRLVG

Sequences:

>Translated_216_residues
MEKFNVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIVAPSFADIFFNNCYKNGLLPIVLTEQQVDHLFNDTYAFNGYQLTIDLDAQVVR
APDGREYPFEITAFRKYCLLNGFDDIGLTLRHADKIRQFEAERLAKQPWLDNRLVG
>Mature_216_residues
MEKFNVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIVAPSFADIFFNNCYKNGLLPIVLTEQQVDHLFNDTYAFNGYQLTIDLDAQVVR
APDGREYPFEITAFRKYCLLNGFDDIGLTLRHADKIRQFEAERLAKQPWLDNRLVG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=211, Percent_Identity=57.8199052132701, Blast_Score=239, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321429, Length=215, Percent_Identity=48.3720930232558, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BURM7 (A3MBT7)

Other databases:

- EMBL:   CP000547
- RefSeq:   YP_001077723.1
- ProteinModelPortal:   A3MBT7
- SMR:   A3MBT7
- GeneID:   4889699
- GenomeReviews:   CP000547_GR
- KEGG:   bmn:BMA10247_A0522
- TIGR:   BMA10247_A0522
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   BMAL320389:BMA10247_A0522-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24726; Mature: 24726

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFNVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK
CCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALQQYGFRAIVAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
YKNGLLPIVLTEQQVDHLFNDTYAFNGYQLTIDLDAQVVRAPDGREYPFEITAFRKYCLL
HHCCCEEEEECHHHHHHHHCCCEEECCEEEEEECCEEEEECCCCCCCCEEHHHHHHHHHH
NGFDDIGLTLRHADKIRQFEAERLAKQPWLDNRLVG
CCCCHHCEEHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKFNVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK
CCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALQQYGFRAIVAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
YKNGLLPIVLTEQQVDHLFNDTYAFNGYQLTIDLDAQVVRAPDGREYPFEITAFRKYCLL
HHCCCEEEEECHHHHHHHHCCCEEECCEEEEEECCEEEEECCCCCCCCEEHHHHHHHHHH
NGFDDIGLTLRHADKIRQFEAERLAKQPWLDNRLVG
CCCCHHCEEHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA