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The map label for this gene is puuB [H]

Identifier: 126446303

GI number: 126446303

Start: 1576597

End: 1577898

Strand: Direct

Name: puuB [H]

Synonym: BMA10247_A1637

Alternate gene names: 126446303

Gene position: 1576597-1577898 (Clockwise)

Preceding gene: 126445646

Following gene: 126446943

Centisome position: 67.01

GC content: 72.96

Gene sequence:

>1302_bases
ATGCAGAACTACTACGAAGCCACGGTCACGCGCACGCCCTACCCCGCGCTCGCCGAACGCATCGATACCGACGTGTGCAT
CGTCGGCGGCGGCCTGGCCGGCCTGTCGACAGCCCTCGGGCTCGTCGAGCGCGGCGTGCGCGACATTGCGCTGCTCGACG
CGCAAACGGTCGGCTTCGGCGCGTCGGGCCGCAACGGCGGCTTCGTGTTCGGCGGCTATAGCCTCGACAACGCGGACCTG
CTGCGCACGCTCGGCCCCGAGTCCGCCCGCGCGCTCTATCGCCTGACGATCGACGCGGTCGAGCTGATCCGCGCGCGCGC
CGCGCGCTACGGCATCGATTGCGAACTCGTCGACGGCGGCGTGATCCTCGCGAACTGGTTCGACGATCCGGCGCGCCTTT
GCGCGCCGCGCAAGCTGATGAAGGAGGCGTTCGGCGTCGACTGGGAGCCGCTCGATGAACGCGCACTGCGCACGCGGCTG
AAGACGGACCGCTATCGCGGCGGCCTCTTCGAGCGCAACGCGTTCCACTTCCATCCGCTGAAGTACGTGCTCGGCGTCGC
GGCCGCGGCCGCGCAGGGCGGCGCACGGATCCACGAGCATTCGGCCGCGCGCGCGATTCGCCGCGACGGCGCGGGTTTTG
TCGTCGCGACCGACGGCGGCGCGGTGCGCGCACGCCGCGTGGTGTTCGCGGGCGGCGGCTATGCGCGCGGCGTGTATCCG
CGCATCGAGCGCGCGGTGCTGCCGATCGCGACCTACGTGATCGCGACCGAGCCGCTCGGCGCGCGGCTCGCCGACGCGAT
CGCGTGCGAGGCGGCCGTCTACGACACCCGCTTCGCGTTCGACTACTACCGGCCGCTCGCCGACACGCGAATTCTGTGGG
GCGGCCGCATTTCGGTGCTCGAACGCGGGCCGGAAGCAATCGCGCGGCTGCTCAGGCGCGACCTGCTGCGCGTCTATCCG
CAACTGCGCGATGCCGCCGTGCAGTTCGCGTGGGGCGGGCTCATGAGCTACGCGCGCCACAAGATGCCGCAGATCGGCCG
CGATGCGCACGGCATCTGGCACGCGGTCGGCTTCGGCGGCCACGGCATGGCGCCGACGACGGTGGCGGGCGAGGTGCTCG
CGGCGGCGCTCGCGGGCGAGCGGCCCGTGCCGGACGGCTTCGCGAGGTTCGGCCTCGCGCGCACGTTCGGGCTCGCGGGG
CTCGCCGCCGCGCAGCTCACGTACAGCGCGTACGAGGCGCGCGACGCGCTCGCCGCGCGCCGCTGCGCACGCGCCGGCCA
GCCCTTTCGTCAGCGCCTTTAG

Upstream 100 bases:

>100_bases
GTCCATCCGTGCAGACGCGGCTTCCTCGCAGGCAAGACAACCGATACGATAATCCGCTCATCCGCTCTCGAGCGGCCCGC
ACGGTCCCAACGACATCCGC

Downstream 100 bases:

>100_bases
AGCCCGGAGTCGGGCAAAAGCCGCCTGGGGCGCGTGCTAGAATGCGCCGACCATGCCGCCGTCCGATCACGCCAAAATGA
AACAAGGAAGCAAGGCCGCA

Product: oxidoreductase, FAD-binding

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQNYYEATVTRTPYPALAERIDTDVCIVGGGLAGLSTALGLVERGVRDIALLDAQTVGFGASGRNGGFVFGGYSLDNADL
LRTLGPESARALYRLTIDAVELIRARAARYGIDCELVDGGVILANWFDDPARLCAPRKLMKEAFGVDWEPLDERALRTRL
KTDRYRGGLFERNAFHFHPLKYVLGVAAAAAQGGARIHEHSAARAIRRDGAGFVVATDGGAVRARRVVFAGGGYARGVYP
RIERAVLPIATYVIATEPLGARLADAIACEAAVYDTRFAFDYYRPLADTRILWGGRISVLERGPEAIARLLRRDLLRVYP
QLRDAAVQFAWGGLMSYARHKMPQIGRDAHGIWHAVGFGGHGMAPTTVAGEVLAAALAGERPVPDGFARFGLARTFGLAG
LAAAQLTYSAYEARDALAARRCARAGQPFRQRL

Sequences:

>Translated_433_residues
MQNYYEATVTRTPYPALAERIDTDVCIVGGGLAGLSTALGLVERGVRDIALLDAQTVGFGASGRNGGFVFGGYSLDNADL
LRTLGPESARALYRLTIDAVELIRARAARYGIDCELVDGGVILANWFDDPARLCAPRKLMKEAFGVDWEPLDERALRTRL
KTDRYRGGLFERNAFHFHPLKYVLGVAAAAAQGGARIHEHSAARAIRRDGAGFVVATDGGAVRARRVVFAGGGYARGVYP
RIERAVLPIATYVIATEPLGARLADAIACEAAVYDTRFAFDYYRPLADTRILWGGRISVLERGPEAIARLLRRDLLRVYP
QLRDAAVQFAWGGLMSYARHKMPQIGRDAHGIWHAVGFGGHGMAPTTVAGEVLAAALAGERPVPDGFARFGLARTFGLAG
LAAAQLTYSAYEARDALAARRCARAGQPFRQRL
>Mature_433_residues
MQNYYEATVTRTPYPALAERIDTDVCIVGGGLAGLSTALGLVERGVRDIALLDAQTVGFGASGRNGGFVFGGYSLDNADL
LRTLGPESARALYRLTIDAVELIRARAARYGIDCELVDGGVILANWFDDPARLCAPRKLMKEAFGVDWEPLDERALRTRL
KTDRYRGGLFERNAFHFHPLKYVLGVAAAAAQGGARIHEHSAARAIRRDGAGFVVATDGGAVRARRVVFAGGGYARGVYP
RIERAVLPIATYVIATEPLGARLADAIACEAAVYDTRFAFDYYRPLADTRILWGGRISVLERGPEAIARLLRRDLLRVYP
QLRDAAVQFAWGGLMSYARHKMPQIGRDAHGIWHAVGFGGHGMAPTTVAGEVLAAALAGERPVPDGFARFGLARTFGLAG
LAAAQLTYSAYEARDALAARRCARAGQPFRQRL

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=392, Percent_Identity=36.4795918367347, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46834; Mature: 46834

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYYEATVTRTPYPALAERIDTDVCIVGGGLAGLSTALGLVERGVRDIALLDAQTVGFG
CCCCEEEEEECCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCHHHHHHCCCHHCCCC
ASGRNGGFVFGGYSLDNADLLRTLGPESARALYRLTIDAVELIRARAARYGIDCELVDGG
CCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
VILANWFDDPARLCAPRKLMKEAFGVDWEPLDERALRTRLKTDRYRGGLFERNAFHFHPL
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECHH
KYVLGVAAAAAQGGARIHEHSAARAIRRDGAGFVVATDGGAVRARRVVFAGGGYARGVYP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCCCCH
RIERAVLPIATYVIATEPLGARLADAIACEAAVYDTRFAFDYYRPLADTRILWGGRISVL
HHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEHHH
ERGPEAIARLLRRDLLRVYPQLRDAAVQFAWGGLMSYARHKMPQIGRDAHGIWHAVGFGG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCC
HGMAPTTVAGEVLAAALAGERPVPDGFARFGLARTFGLAGLAAAQLTYSAYEARDALAAR
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RCARAGQPFRQRL
HHHHCCCHHHHCC
>Mature Secondary Structure
MQNYYEATVTRTPYPALAERIDTDVCIVGGGLAGLSTALGLVERGVRDIALLDAQTVGFG
CCCCEEEEEECCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCHHHHHHCCCHHCCCC
ASGRNGGFVFGGYSLDNADLLRTLGPESARALYRLTIDAVELIRARAARYGIDCELVDGG
CCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
VILANWFDDPARLCAPRKLMKEAFGVDWEPLDERALRTRLKTDRYRGGLFERNAFHFHPL
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECHH
KYVLGVAAAAAQGGARIHEHSAARAIRRDGAGFVVATDGGAVRARRVVFAGGGYARGVYP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCCCCH
RIERAVLPIATYVIATEPLGARLADAIACEAAVYDTRFAFDYYRPLADTRILWGGRISVL
HHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEHHH
ERGPEAIARLLRRDLLRVYPQLRDAAVQFAWGGLMSYARHKMPQIGRDAHGIWHAVGFGG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCC
HGMAPTTVAGEVLAAALAGERPVPDGFARFGLARTFGLAGLAAAQLTYSAYEARDALAAR
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RCARAGQPFRQRL
HHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]