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The map label for this gene is kgtP [H]

Identifier: 126446216

GI number: 126446216

Start: 68509

End: 69861

Strand: Direct

Name: kgtP [H]

Synonym: BMA10247_A0083

Alternate gene names: 126446216

Gene position: 68509-69861 (Clockwise)

Preceding gene: 262192846

Following gene: 126447915

Centisome position: 2.91

GC content: 63.27

Gene sequence:

>1353_bases
ATGGAGCCGACCTTTACCGAAACCGGCGCGCGGGCGAGCGAGCCCCACGCAACAACGCTTGCCGGCCGAGCCGCATCGTC
CGGCGATAAGCTGCAACGCCTGAAGTCGATCTTCTCCGCATCGGCGGGCAATCTGATCGAGTGGTACGACTGGTACGTGT
ATTCGGCGTTTGCGCTGTACTTCGCGCATTCGTTTTTTCCGGCCGGCAACCAGACCGCGCAACTGCTCAACACGGCCGCC
GTCTTCGCGGTGGGCTTTCTGGCGCGCCCCGTCGGCGGCTGGCTGATGGGGATCTACGCGGATCGCTACGGCCGCCGTCC
GGCCTTGCTCGTATCGGTCGTGCTGATGTGCGTGGGCTCGCTCATCATCGCCCTCTGCCCGGGGTATGACGTGATCGGCA
CGGCCGCGCCTGTCCTGCTCGTCGTCGCCCGCCTGCTGCAGGGCTTGAGCGTCGGGGGCGAATACGGCACGTCGGCGACC
TATCTCAGCGAAGTCGCGACCGCCCGCGATCGCGGCTTCTACTCCAGCTTTCAGTACGTCACGCTGGTTGCCGGCCAACT
CGTCGCGCTGGCGCTGCTGATCGTCCTGCAGCAATTCGTGCTGACGACACAGCAGCTCGAAAGCTGGGGGTGGAGAATTC
CGTTCTTCATCGGCGCGGCGTGCGCGCTGGCCGCCATGCGCCTGCGCTCGTCGATGGAGGAAACGGGCGAATTCAAGCGG
ACCTTAAACGCCCGGGACAAGCGCGGCACGCTCGCGGAACTGAGCAAGCATCCGCGCGCGGTCCTGACCGTGGTCGGCCT
GACGATGGGCGGCACGATCGCGTTCTACACCTACTCGATCTACATGCAGAAATTCCTCGTGAACACCGTCGGCATGAGCA
AACATGATGCGACCCTGGTATCCGCCGCGTCGCTCGCCCTGTTCGCGATCTTGCAGCCGATCGTCGGCTCGATCTCCGAT
CGCATCGGCCGTCGCCCCGTGCTGATCGCGTTCGGCGTGCTCGGCACGTTGTTCACCGTGCCGATCATGACGGCCATCAG
CCGGACTCATGACGTTTGGACCGCGTTCTTCCTCAACATGGCGGCGCTGGTGATCGTGTCGGGGTATACCTCGATCAACG
CAGTCGTGAAGGCGGAATTGTTCCCTGCGAAAATCCGTGCACTGGGCGTCGGCTTTCCGTATGCACTCACCGTGTCGATC
TTCGGCGGCACGGCGGAGTATCTGGCGCTCTGGCTCAAGCAGGCTGGCCACGAATCGCTGTTCTATTGGTACGTGACAGC
CGCCATATTCTGTTCGTTGCTCGTCTACGTATGCATGCGGGATACCGGGAAGCACTCGCTGATCAAGGATTGA

Upstream 100 bases:

>100_bases
AGAGGCTTATGAAGGGCTCGACAGATCGGCTCGTTGGCACTGTTGTTGCAAGAGGATGGCACCGCATCGAGCGGCATTCG
ACAAAACACCAGGAGACGCC

Downstream 100 bases:

>100_bases
TGGCGCCGGCCGCCACCGTGCCGACGGCGCCCGCGCACGAGGATGCCGCGCGATGAGGCTGCGCGGCATCCGGCGGCTGC
ACCGGCCGGCGGCGGTGCTC

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MEPTFTETGARASEPHATTLAGRAASSGDKLQRLKSIFSASAGNLIEWYDWYVYSAFALYFAHSFFPAGNQTAQLLNTAA
VFAVGFLARPVGGWLMGIYADRYGRRPALLVSVVLMCVGSLIIALCPGYDVIGTAAPVLLVVARLLQGLSVGGEYGTSAT
YLSEVATARDRGFYSSFQYVTLVAGQLVALALLIVLQQFVLTTQQLESWGWRIPFFIGAACALAAMRLRSSMEETGEFKR
TLNARDKRGTLAELSKHPRAVLTVVGLTMGGTIAFYTYSIYMQKFLVNTVGMSKHDATLVSAASLALFAILQPIVGSISD
RIGRRPVLIAFGVLGTLFTVPIMTAISRTHDVWTAFFLNMAALVIVSGYTSINAVVKAELFPAKIRALGVGFPYALTVSI
FGGTAEYLALWLKQAGHESLFYWYVTAAIFCSLLVYVCMRDTGKHSLIKD

Sequences:

>Translated_450_residues
MEPTFTETGARASEPHATTLAGRAASSGDKLQRLKSIFSASAGNLIEWYDWYVYSAFALYFAHSFFPAGNQTAQLLNTAA
VFAVGFLARPVGGWLMGIYADRYGRRPALLVSVVLMCVGSLIIALCPGYDVIGTAAPVLLVVARLLQGLSVGGEYGTSAT
YLSEVATARDRGFYSSFQYVTLVAGQLVALALLIVLQQFVLTTQQLESWGWRIPFFIGAACALAAMRLRSSMEETGEFKR
TLNARDKRGTLAELSKHPRAVLTVVGLTMGGTIAFYTYSIYMQKFLVNTVGMSKHDATLVSAASLALFAILQPIVGSISD
RIGRRPVLIAFGVLGTLFTVPIMTAISRTHDVWTAFFLNMAALVIVSGYTSINAVVKAELFPAKIRALGVGFPYALTVSI
FGGTAEYLALWLKQAGHESLFYWYVTAAIFCSLLVYVCMRDTGKHSLIKD
>Mature_450_residues
MEPTFTETGARASEPHATTLAGRAASSGDKLQRLKSIFSASAGNLIEWYDWYVYSAFALYFAHSFFPAGNQTAQLLNTAA
VFAVGFLARPVGGWLMGIYADRYGRRPALLVSVVLMCVGSLIIALCPGYDVIGTAAPVLLVVARLLQGLSVGGEYGTSAT
YLSEVATARDRGFYSSFQYVTLVAGQLVALALLIVLQQFVLTTQQLESWGWRIPFFIGAACALAAMRLRSSMEETGEFKR
TLNARDKRGTLAELSKHPRAVLTVVGLTMGGTIAFYTYSIYMQKFLVNTVGMSKHDATLVSAASLALFAILQPIVGSISD
RIGRRPVLIAFGVLGTLFTVPIMTAISRTHDVWTAFFLNMAALVIVSGYTSINAVVKAELFPAKIRALGVGFPYALTVSI
FGGTAEYLALWLKQAGHESLFYWYVTAAIFCSLLVYVCMRDTGKHSLIKD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=51.5151515151515, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1790550, Length=442, Percent_Identity=31.447963800905, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=30.2439024390244, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=29.5774647887324, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI87082231, Length=179, Percent_Identity=31.2849162011173, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87082404, Length=378, Percent_Identity=24.0740740740741, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48764; Mature: 48764

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTFTETGARASEPHATTLAGRAASSGDKLQRLKSIFSASAGNLIEWYDWYVYSAFALY
CCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FAHSFFPAGNQTAQLLNTAAVFAVGFLARPVGGWLMGIYADRYGRRPALLVSVVLMCVGS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LIIALCPGYDVIGTAAPVLLVVARLLQGLSVGGEYGTSATYLSEVATARDRGFYSSFQYV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
TLVAGQLVALALLIVLQQFVLTTQQLESWGWRIPFFIGAACALAAMRLRSSMEETGEFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLNARDKRGTLAELSKHPRAVLTVVGLTMGGTIAFYTYSIYMQKFLVNTVGMSKHDATLV
HHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SAASLALFAILQPIVGSISDRIGRRPVLIAFGVLGTLFTVPIMTAISRTHDVWTAFFLNM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALVIVSGYTSINAVVKAELFPAKIRALGVGFPYALTVSIFGGTAEYLALWLKQAGHESL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHH
FYWYVTAAIFCSLLVYVCMRDTGKHSLIKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEPTFTETGARASEPHATTLAGRAASSGDKLQRLKSIFSASAGNLIEWYDWYVYSAFALY
CCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FAHSFFPAGNQTAQLLNTAAVFAVGFLARPVGGWLMGIYADRYGRRPALLVSVVLMCVGS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LIIALCPGYDVIGTAAPVLLVVARLLQGLSVGGEYGTSATYLSEVATARDRGFYSSFQYV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
TLVAGQLVALALLIVLQQFVLTTQQLESWGWRIPFFIGAACALAAMRLRSSMEETGEFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLNARDKRGTLAELSKHPRAVLTVVGLTMGGTIAFYTYSIYMQKFLVNTVGMSKHDATLV
HHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SAASLALFAILQPIVGSISDRIGRRPVLIAFGVLGTLFTVPIMTAISRTHDVWTAFFLNM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALVIVSGYTSINAVVKAELFPAKIRALGVGFPYALTVSIFGGTAEYLALWLKQAGHESL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHH
FYWYVTAAIFCSLLVYVCMRDTGKHSLIKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]