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The map label for this gene is truA

Identifier: 126446203

GI number: 126446203

Start: 474426

End: 475241

Strand: Direct

Name: truA

Synonym: BMA10247_A0527

Alternate gene names: 126446203

Gene position: 474426-475241 (Clockwise)

Preceding gene: 126445675

Following gene: 126447841

Centisome position: 20.17

GC content: 68.01

Gene sequence:

>816_bases
GTGATGAGAATCGCACTCGGCATTCAGTACGACGGCGCGGCGTTCTGCGGCTGGCAGTCGCAGCCCCACGGCAAGACGGT
TCAGGACGCGCTCGAGCGCTCGCTCGCGGAATTCGCGCAGACGTCGCTGCACACGACGGTCGCGGGCCGCACCGACACCG
GCGTGCACGGCCTCGGCCAGGTCGTCCATTTCGATACCGACCTCGACCGCGCGGATTTCTCATGGGTGCGCGGCACGAAC
GCGTTCCTGCCGCCGACGGTCGCCGTGCAGTGGGCGAAGCCGATGCCGGATACGTTCCACGCGCGCTTCGCCGCTTTCGA
GCGCACCTACTACTACGCGCTGTACGTGCATCCGGTGCGCTCGCCGATGCTCGCGGGGCGCGCGGGGTGGGTCCACACGC
CGCTCGACGTCGACGCAATGCGCGAAGCGGCCGCGCATCTCGTCGGCGAGCACGATTTCTCGGCGTTTCGCTCGTCCGAG
TGCCAGGCGAAATCGCCCGTCAAGCATCTGTACCAGATCGGCATCCGCCCCGACGGCGATTTCATCCACTTCCGCTTCCG
CGCGAACGCGTTCCTGCACCACATGGTGCGCAATCTGATGGGCTGCCTCGTCGCGGTGGGGCGCGGCCGCTATCCGTCGT
CGTGGCTCGCCGAAGTGCTGGAGAGCCGCGATCGCGATTGCGCGGCGCCGACTTTCATGCCCGAAGGGCTCTATCTCGCG
CACGTCGGCTATCCGGCCGAATTCGCGGTGCCGCCCGCACAGCTCGGCAGCGTGCCGTGGAGCAGCGTGTGGGCCGATCT
GGACGGACGAACATGA

Upstream 100 bases:

>100_bases
TGGCCGGCGCGCGCACGCTGCTGCACGAAGTGATCGAAGCCGATCACGCCGACACGCGCGACGAAGCGCGCGTGATGCTC
GCGAAACTCGCCGATCTGTC

Downstream 100 bases:

>100_bases
TGACCGATACACCCGATGCGACCCTTCCGGCCGACGCGCCGCCGAACGACGCGCCCGCCGCCGGCGCGCGCCGCGACACG
GGCGGCGACGGGAGTCGCGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MMRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTN
AFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSE
CQAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLA
HVGYPAEFAVPPAQLGSVPWSSVWADLDGRT

Sequences:

>Translated_271_residues
MMRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTN
AFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSE
CQAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLA
HVGYPAEFAVPPAQLGSVPWSSVWADLDGRT
>Mature_271_residues
MMRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTN
AFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSE
CQAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLA
HVGYPAEFAVPPAQLGSVPWSSVWADLDGRT

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=244, Percent_Identity=31.9672131147541, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=44.8818897637795, Blast_Score=240, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI71985046, Length=261, Percent_Identity=25.6704980842912, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BURM7 (A3MBU2)

Other databases:

- EMBL:   CP000547
- RefSeq:   YP_001077728.1
- ProteinModelPortal:   A3MBU2
- SMR:   A3MBU2
- GeneID:   4890669
- GenomeReviews:   CP000547_GR
- KEGG:   bmn:BMA10247_A0527
- TIGR:   BMA10247_A0527
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   BMAL320389:BMA10247_A0527-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30145; Mature: 30145

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQ
CEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCC
VVHFDTDLDRADFSWVRGTNAFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVR
EEEECCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCC
SPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSECQAKSPVKHLYQIGIRPDGD
CCHHCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
FIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLA
EEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEE
HVGYPAEFAVPPAQLGSVPWSSVWADLDGRT
EECCCCCCCCCHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MMRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQ
CEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCC
VVHFDTDLDRADFSWVRGTNAFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVR
EEEECCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCC
SPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSECQAKSPVKHLYQIGIRPDGD
CCHHCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
FIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLA
EEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEE
HVGYPAEFAVPPAQLGSVPWSSVWADLDGRT
EECCCCCCCCCHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA