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The map label for this gene is braC [H]

Identifier: 126445864

GI number: 126445864

Start: 1704354

End: 1705496

Strand: Reverse

Name: braC [H]

Synonym: BMA10247_A1769

Alternate gene names: 126445864

Gene position: 1705496-1704354 (Counterclockwise)

Preceding gene: 126447448

Following gene: 126446384

Centisome position: 72.49

GC content: 65.44

Gene sequence:

>1143_bases
GTGAAAAGGAAAATCAACAGACTGTTGCCGATCAGCGCGGCCGCGATGGTGATGGCCGCGGCCTCGACGCATGCGCTCGC
CGATCAGGTCGTGAAGATCGGCAGTGCCGAGCCCTTGACGGGCGGCATCGCCCACCTCGGCAAGGACAACGAGAACGGCG
CGCGTCTCGCGGTCGAGGAGATCAATGCGAAGGGCCTCACGATCGGCGGCCAGAAGGTCACGCTCCAGCTCGACGCGCAG
GACGACGCGGCCGACCCGCGCACCGCGACGCAGGTCGCGCAGAAGCTCGTCGACGGCAAGGTCGTCGCGGTGATCGGCCA
CCTGAATTCGGGAACGTCGATTCCGGCCTCGAAGATCTACAGCGATGCCGGCATCCTGCAGATCTCGCCGTCGGCGACGA
ACCCGGCCTACACGCAACAAGGCTTCAAGACGACCTACCGCGTCGTCGCGACCGACGCGCAGCAAGGCCCGGCGCTCGCC
GACTACGCGAAGCAGAAGGGCATCAAGACGGTGGCCGTCGTCGACGATTCGACGGCCTACGGCCAGGGCCTCGCGAACGA
ATTCGAGAAGAAGGCGAAGGCGCTCGGCCTGAAGGTGGTGTCGCACGACGCGACCAACGACAAGGCGGTCGATTTCCGCG
CGATCCTGACGAAGATCAAGGGTACGAACCCGGACGCGATCATGTACGGCGGCATGGACGCGACGGGCGGCCCGTTCGCG
AAGCAGGCGAAGCAGCTCGGCCTGCGCGCGAAGATCTTCGCGGGCGACGGCGTGTGCACCGAGCAGTTGCCGGCGCTCGC
CGGCAACGCGGCCGAGAACGTCGTGTGCTCGCAGGCGGGCGCGGCGCTCGAGAAGATGCCGGGCGGCGCGACGTTCCAGG
CCAAGTACGAGAAGCGCTTCAACCAGCCGATCCGCTTCGACGCGCCGTTCACGTATGACGCGGTGTACATCATCGTCGAC
GCGATGAAGCGCGCGAACTCGACGGACCCGGCGAAGATCCTCGCGGTGATGCCGAAGACGAACTACACCGGCGTGATCGG
CACGACGGTCTTCGATTCGAAGGGTGACCTGAAGCACGGCGTGATTTCGCTGTACGACTTCAAGGGCGGCAAGAAGACCT
TCCTCGATCAGGTGACGATGTAA

Upstream 100 bases:

>100_bases
GCAGCCTGCGAACGAACCGGCGCACGCGGCGCGCTGCAACGAATTTTGAACGGGTATCATCCGATCATATGATCTACGCC
CGTTCAACAAGGAGACTGCA

Downstream 100 bases:

>100_bases
GCGCGACGGGCGGCGTTTGCCGCTCATTGCGCCGATCGATGCGACGGGCGGACGCCGGATTGGGCGTCCGCCCGTTTTGT
TGTCCGTTTGGTTATCCGTT

Product: putative amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKRKINRLLPISAAAMVMAAASTHALADQVVKIGSAEPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDGKVVAVIGHLNSGTSIPASKIYSDAGILQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
DYAKQKGIKTVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGTNPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEQLPALAGNAAENVVCSQAGAALEKMPGGATFQAKYEKRFNQPIRFDAPFTYDAVYIIVD
AMKRANSTDPAKILAVMPKTNYTGVIGTTVFDSKGDLKHGVISLYDFKGGKKTFLDQVTM

Sequences:

>Translated_380_residues
MKRKINRLLPISAAAMVMAAASTHALADQVVKIGSAEPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDGKVVAVIGHLNSGTSIPASKIYSDAGILQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
DYAKQKGIKTVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGTNPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEQLPALAGNAAENVVCSQAGAALEKMPGGATFQAKYEKRFNQPIRFDAPFTYDAVYIIVD
AMKRANSTDPAKILAVMPKTNYTGVIGTTVFDSKGDLKHGVISLYDFKGGKKTFLDQVTM
>Mature_380_residues
MKRKINRLLPISAAAMVMAAASTHALADQVVKIGSAEPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDGKVVAVIGHLNSGTSIPASKIYSDAGILQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
DYAKQKGIKTVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGTNPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEQLPALAGNAAENVVCSQAGAALEKMPGGATFQAKYEKRFNQPIRFDAPFTYDAVYIIVD
AMKRANSTDPAKILAVMPKTNYTGVIGTTVFDSKGDLKHGVISLYDFKGGKKTFLDQVTM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=359, Percent_Identity=33.983286908078, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI48994941, Length=355, Percent_Identity=32.3943661971831, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39922; Mature: 39922

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKINRLLPISAAAMVMAAASTHALADQVVKIGSAEPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKVTLQLDAQDDAADPRTATQVAQKLVDGKVVAVIGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHH
SDAGILQISPSATNPAYTQQGFKTTYRVVATDAQQGPALADYAKQKGIKTVAVVDDSTAY
CCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGTNPDAIMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEQLPALAGNAAENVVCSQAGAALEKMPGGATFQAKYEKRF
HHHHHCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHCCCCCEEEEHHHHHC
NQPIRFDAPFTYDAVYIIVDAMKRANSTDPAKILAVMPKTNYTGVIGTTVFDSKGDLKHG
CCCEEECCCCCCCHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCC
VISLYDFKGGKKTFLDQVTM
EEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MKRKINRLLPISAAAMVMAAASTHALADQVVKIGSAEPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKVTLQLDAQDDAADPRTATQVAQKLVDGKVVAVIGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHH
SDAGILQISPSATNPAYTQQGFKTTYRVVATDAQQGPALADYAKQKGIKTVAVVDDSTAY
CCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGTNPDAIMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEQLPALAGNAAENVVCSQAGAALEKMPGGATFQAKYEKRF
HHHHHCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHCCCCCEEEEHHHHHC
NQPIRFDAPFTYDAVYIIVDAMKRANSTDPAKILAVMPKTNYTGVIGTTVFDSKGDLKHG
CCCEEECCCCCCCHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCC
VISLYDFKGGKKTFLDQVTM
EEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]