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The map label for this gene is alaXM [H]

Identifier: 126445649

GI number: 126445649

Start: 1343065

End: 1343784

Strand: Reverse

Name: alaXM [H]

Synonym: BMA10247_A1375

Alternate gene names: 126445649

Gene position: 1343784-1343065 (Counterclockwise)

Preceding gene: 126447290

Following gene: 126445779

Centisome position: 57.12

GC content: 69.58

Gene sequence:

>720_bases
ATGAATCGATTGTTCTGTCAAGACGACGCCGACACGCTGCTGCTTTCGGGCACGGTGGTCGACACGCGGCCGGGCGCGGT
GCGCCTGTCGCGGCATCCGTTCTATCCGGGCGGCGGCGGCCAGTTGCCCGATCTGGGCACGGTTCGATGGGAGGGCGGCG
TCGAGACCGTCGCCGGTTTCAGCGAGGACGGCGGCGGCCTCTGGATCGAATTCGCGAACGGCGTCGAGATCGTCGGCGAC
GTGCAGTCGAGCGTCGACGCCGCGTTCCGCCGCGCGATGCGCGAGCTGCATACCGATCTGCACGTGCTCAACGCGCTCGT
CTATCGCGATTTCGACGGCGCGCTCGTGACGGGCGTGCAGATGAACGCCGATCGCACCGCGCGCATCGATTTCGATCTGC
CCGACGCCGATAACGATCGCCTGCGCGCGCTGGAGGCCGAAATGAACGATGTCGTGCGCCGCGATCTGCCGGTGCGCACG
CGCTACGTGCCGCTCGCCGACGCGCAGCAGGCAAGCGGGCTGCTGCGCTCGCGCTCGGTCGCGCCGCCGCCGACGGCCGA
CGGGCTCGTGCGGATCGTGGAGATCGACGGGCTCGACAGCCAGGCGTGCGGCGGCACGCATCTCGCGTCGACGGGCGGCT
CGAGCCCGGTGCGGATCCTGAAGATCGAAAACAAGGGCCGCTCGAACCGCCGCGTGCGCATCGGGCTGGAGCCGAGATGA

Upstream 100 bases:

>100_bases
GAAGCGCCCGGCGGCGAGCGGGCGTCGCGCGCCGCCAAATTTTCGCCAATAAGCCCGCTTCGACTTCTGACATCTTTCAT
TCATCAGCCAGGTGGAGCTT

Downstream 100 bases:

>100_bases
CGCAGCGCCTGCTCGCGGCGGCATGGCGCCGGCCGGTGTGGCTGCTGTGGGTGCCGCCCGCGTTCTGGGCCGGCAACGTC
GTGCTTGGCCGCGCGTTCAG

Product: alanyl-tRNA synthetase-related protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNRLFCQDDADTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR

Sequences:

>Translated_239_residues
MNRLFCQDDADTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
>Mature_239_residues
MNRLFCQDDADTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGFSEDGGGLWIEFANGVEIVGD
VQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQMNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRT
RYVPLADAQQASGLLRSRSVAPPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=231, Percent_Identity=28.1385281385281, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 25885; Mature: 25885

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLFCQDDADTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGF
CCCEECCCCCCEEEEECEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHCCC
SEDGGGLWIEFANGVEIVGDVQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQ
CCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRTRYVPLADAQQASGLLRSRSV
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCC
APPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
CCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
>Mature Secondary Structure
MNRLFCQDDADTLLLSGTVVDTRPGAVRLSRHPFYPGGGGQLPDLGTVRWEGGVETVAGF
CCCEECCCCCCEEEEECEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHCCC
SEDGGGLWIEFANGVEIVGDVQSSVDAAFRRAMRELHTDLHVLNALVYRDFDGALVTGVQ
CCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
MNADRTARIDFDLPDADNDRLRALEAEMNDVVRRDLPVRTRYVPLADAQQASGLLRSRSV
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCC
APPPTADGLVRIVEIDGLDSQACGGTHLASTGGSSPVRILKIENKGRSNRRVRIGLEPR
CCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]