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The map label for this gene is wcaJ [H]

Identifier: 126455454

GI number: 126455454

Start: 634541

End: 635923

Strand: Direct

Name: wcaJ [H]

Synonym: BURPS1106A_0660

Alternate gene names: 126455454

Gene position: 634541-635923 (Clockwise)

Preceding gene: 126452554

Following gene: 126455396

Centisome position: 15.91

GC content: 67.25

Gene sequence:

>1383_bases
ATGGTGGGACTTTTGAATAAATTGCTCGATGTGGCGCTGGTCGCGGTGGGCGCGCTGCTGGCGCAGATGCTGTACGACGG
CGGCCTCTTCGATGTGTCCGACGCGCAGCGCACGGCCATCGCGCTGCTGTGCGCGCTGACGCTGCTCGTCTTTGCGGCGA
TCGGCGTCTACGACGGCGCACGCGAGCAGATGTCGTATCGCACGCTCTCGCGCGTGCTGCTCGGCTGGCTCGGCGTCGCG
GCGGCCGCCGCGGCCTTCACGCTGCTGCTGCAGCGTGACGCCGACGTGTCGCTCGCGTGGCTCGGCCGCACGATGCTGAT
GTCCGGCGCGGTGCTGCTGCTCGGCAGGGCGTCGATCCATGGCGTGCTGAACGGCCTGCGCCGCACGGGCGCGAAGCCGC
GGGCAGTCGCGATCGTCGGCTCCGAAGTCTATGGCCGCGCGGTGATCGAGCAACTGCGCGCGTCGTCGCTGCACGGCTAC
GAGGCTGTCTGCGTGTTCGACGACAGCGCGCGCGCGGCCGATGCCGAGCTTCGCGTGGGCGGCGTGCCGGTCGTCTCCGA
TCTGCGCGAGTTCAAGCGCCGCGTGCGCGCGGGTGCGATCAAGGAAATCTGGCTCGCGCTGCCGCTGTCGCACGAGCGAC
GGATTCAGCGGATCGTGCGCGAGTTTCGCCACGATTTCGTGAACCTGCGCTTCCTGCCCGACGTGCGCGGGATCACGTTC
TTCAACCGCTCGGTCGCGCAGGTCGCGGGCATGCCCGCGATCAACCTCGCGACGAGCCCGCTGTCGATTCCGCAGCTCTG
GCCGAAGTTCATCTTCGATCGCCTGTTCGCGCTCGCCGTGCTGATTCCGCTGTCGCCGATCCTCGCGGCGCTCGCGCTCG
CGGTGAAGCTCTCGTCGCCGGGGCCCGTGCTGTTCCGGCAGAAGCGCAAGGGCGTCGACGGCCGCGAGTTCGAGATCCTG
AAGTTCCGCACGATGCGCGTGCACGCCGAGGAGACGGGCGTCGTGCGCCAGGCGTCGCGTAACGATTCGCGCATCACGAA
GGTCGGCGCGTTCCTGCGCCGCACGTCGCTCGACGAGCTGCCGCAGTTCTTCAACGTGCTGTTCGGCCAGATGTCCGTCG
TCGGTCCGCGTCCGCACGCGATCGAGCACGACGACCTCTACAAGGAGCTCGTCGACGGCTACATGTACCGCTATCGCGTG
CGTCCCGGCATCACCGGCTGGGCGCAGGTGAACGGGTATCGAGGCGAGACGCGCAAGGTCGAGAAGATGGCCGCGCGCGT
GAAGTTCGATCTTTTCTACATGCAGAACTGGACCTTCTGGTTCGACATCAAGATCATCCTGATCACGCTCGTCAAGGGTT
TCGTCGGCCGCAACGCATTCTGA

Upstream 100 bases:

>100_bases
CGATTCCGGCGGAGGGCCGGCCGCTGCGTGTCGCCGGCTATCACGGCGCGGTGCGCTCGGCGGCGTGAGACGACGGAACA
CGAAACGCGGAGATTCGGTG

Downstream 100 bases:

>100_bases
GCCGGCGCGCGTTCGCCGCGAACGCGCGGCATTCCTTTTCGACGATCACGCTACCAAGGAGAAACGACAAGATGTTCAAG
CCCCTAGCATGGGCGAGCGC

Product: putative sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MVGLLNKLLDVALVAVGALLAQMLYDGGLFDVSDAQRTAIALLCALTLLVFAAIGVYDGAREQMSYRTLSRVLLGWLGVA
AAAAAFTLLLQRDADVSLAWLGRTMLMSGAVLLLGRASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVIEQLRASSLHGY
EAVCVFDDSARAADAELRVGGVPVVSDLREFKRRVRAGAIKEIWLALPLSHERRIQRIVREFRHDFVNLRFLPDVRGITF
FNRSVAQVAGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKLSSPGPVLFRQKRKGVDGREFEIL
KFRTMRVHAEETGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDLYKELVDGYMYRYRV
RPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF

Sequences:

>Translated_460_residues
MVGLLNKLLDVALVAVGALLAQMLYDGGLFDVSDAQRTAIALLCALTLLVFAAIGVYDGAREQMSYRTLSRVLLGWLGVA
AAAAAFTLLLQRDADVSLAWLGRTMLMSGAVLLLGRASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVIEQLRASSLHGY
EAVCVFDDSARAADAELRVGGVPVVSDLREFKRRVRAGAIKEIWLALPLSHERRIQRIVREFRHDFVNLRFLPDVRGITF
FNRSVAQVAGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKLSSPGPVLFRQKRKGVDGREFEIL
KFRTMRVHAEETGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDLYKELVDGYMYRYRV
RPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
>Mature_460_residues
MVGLLNKLLDVALVAVGALLAQMLYDGGLFDVSDAQRTAIALLCALTLLVFAAIGVYDGAREQMSYRTLSRVLLGWLGVA
AAAAAFTLLLQRDADVSLAWLGRTMLMSGAVLLLGRASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVIEQLRASSLHGY
EAVCVFDDSARAADAELRVGGVPVVSDLREFKRRVRAGAIKEIWLALPLSHERRIQRIVREFRHDFVNLRFLPDVRGITF
FNRSVAQVAGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKLSSPGPVLFRQKRKGVDGREFEIL
KFRTMRVHAEETGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDLYKELVDGYMYRYRV
RPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=470, Percent_Identity=37.8723404255319, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51213; Mature: 51213

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGLLNKLLDVALVAVGALLAQMLYDGGLFDVSDAQRTAIALLCALTLLVFAAIGVYDGA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
REQMSYRTLSRVLLGWLGVAAAAAAFTLLLQRDADVSLAWLGRTMLMSGAVLLLGRASIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
GVLNGLRRTGAKPRAVAIVGSEVYGRAVIEQLRASSLHGYEAVCVFDDSARAADAELRVG
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEC
GVPVVSDLREFKRRVRAGAIKEIWLALPLSHERRIQRIVREFRHDFVNLRFLPDVRGITF
CCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHH
FNRSVAQVAGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKLSSP
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPVLFRQKRKGVDGREFEILKFRTMRVHAEETGVVRQASRNDSRITKVGAFLRRTSLDEL
CCHHHHHHHCCCCCCCEEEEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
PQFFNVLFGQMSVVGPRPHAIEHDDLYKELVDGYMYRYRVRPGITGWAQVNGYRGETRKV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHEECCCCCHHHHH
EKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
HHHHHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVGLLNKLLDVALVAVGALLAQMLYDGGLFDVSDAQRTAIALLCALTLLVFAAIGVYDGA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
REQMSYRTLSRVLLGWLGVAAAAAAFTLLLQRDADVSLAWLGRTMLMSGAVLLLGRASIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
GVLNGLRRTGAKPRAVAIVGSEVYGRAVIEQLRASSLHGYEAVCVFDDSARAADAELRVG
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEC
GVPVVSDLREFKRRVRAGAIKEIWLALPLSHERRIQRIVREFRHDFVNLRFLPDVRGITF
CCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHH
FNRSVAQVAGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKLSSP
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPVLFRQKRKGVDGREFEILKFRTMRVHAEETGVVRQASRNDSRITKVGAFLRRTSLDEL
CCHHHHHHHCCCCCCCEEEEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
PQFFNVLFGQMSVVGPRPHAIEHDDLYKELVDGYMYRYRVRPGITGWAQVNGYRGETRKV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHEECCCCCHHHHH
EKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
HHHHHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]