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The map label for this gene is phnJ [H]

Identifier: 126455305

GI number: 126455305

Start: 3265116

End: 3266090

Strand: Reverse

Name: phnJ [H]

Synonym: BURPS1106A_3342

Alternate gene names: 126455305

Gene position: 3266090-3265116 (Counterclockwise)

Preceding gene: 126453653

Following gene: 126454070

Centisome position: 81.89

GC content: 68.62

Gene sequence:

>975_bases
ATGAACGCGCCCGACCATTGCGCGGCCGCCGCGCCCGACACGCACGCCGCCGAAGGCTACAACTTCGCGTACCTCGACGA
GCAGACGAAGCGGATGACCCGCCGCGCGCTGCTCAAGGCGGTCGCGGTGCCGGGCTATCAGGTGCCGTTCGCGTCGCGCG
AGATGCCGCTGCCGTTCGGCTGGGGAACGGGCGGCATTCAGGTGACGGCCGCGCTGATCGGCCGCGACGATACGCTGAAG
GTGATCGATCAAGGCTCCGACGAAACGACGAACGCGGTCAACATCCGTCGCTTCTTCGCGCGCACCGCGGGCGTCGCGAC
GACGACGAAGACGGCGCAAGCGACGATCATCCAGACGCGCCACCGCGTTCCGGAGACGCCGCTCACCGAGCGTCAGATCC
TCGTCTATCAGGTGCCGATGCCGGAGCCGCTCTTTCGCCTGGAGCCGCGCGCGACCGAATGCGCGAAGCTGCACGCGCAC
GCCGATTACGGGCTGATGCGCGTGAAGCTGTACGAGGACATCGTGCATCACGGCAGCATCGCGACGACGTACGATTACCC
GGTGATCGTCAACGGCCGCTACCTGAGCTCGCCGTCGCCGATCCCGAAGTTCGACAATCCGAAGCTGCACATGAACCCGG
CGCTGCAACTGTTCGGCGCGGGGCGCGAGCGCCGCATCTACGCGATTGCGCCGTACACGCCGGTCGCGAGCCTCGATTTC
GACGATCATCCGTTCGAAGTCCAGCGCTGGGACGCCGCGTGCGCGCTGTGCGGCTCGCGCGAGAGCTTTCTCGACGAGAT
GATCGTCGACGATCACGGCACGCGCCGCTTCGTGTGCTCGGACAGCGACTATTGCAGCGACCGCCGCGGCGGCGCGCAGG
CGCGCGCCGACGGCGGCGACGAGGGCAACGGCGGCGGCGACGGGGAGATGCCCGCGCCGCTCGCGGCCGAACAGCCGCTG
GGAGAGACGACATGA

Upstream 100 bases:

>100_bases
TGCGCGCCGGGCATGCGGCGCAGGCGGGAGCGGGCGCGAACGCGCACGAGAACGCGCCCGCCCGCGCCGAACGCGCCGAC
ACGCACACCGAGGAGTCCCG

Downstream 100 bases:

>100_bases
CGCCGCTTCTGAGCGCGCGCGGGCTGACGAAGCGCTTCGGCGGACGCAACGGTTTCGCGAACGCGAGCTTCGATCTGTAT
CCGGGGGAGGTGCTGTGCAT

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNAPDHCAAAAPDTHAAEGYNFAYLDEQTKRMTRRALLKAVAVPGYQVPFASREMPLPFGWGTGGIQVTAALIGRDDTLK
VIDQGSDETTNAVNIRRFFARTAGVATTTKTAQATIIQTRHRVPETPLTERQILVYQVPMPEPLFRLEPRATECAKLHAH
ADYGLMRVKLYEDIVHHGSIATTYDYPVIVNGRYLSSPSPIPKFDNPKLHMNPALQLFGAGRERRIYAIAPYTPVASLDF
DDHPFEVQRWDAACALCGSRESFLDEMIVDDHGTRRFVCSDSDYCSDRRGGAQARADGGDEGNGGGDGEMPAPLAAEQPL
GETT

Sequences:

>Translated_324_residues
MNAPDHCAAAAPDTHAAEGYNFAYLDEQTKRMTRRALLKAVAVPGYQVPFASREMPLPFGWGTGGIQVTAALIGRDDTLK
VIDQGSDETTNAVNIRRFFARTAGVATTTKTAQATIIQTRHRVPETPLTERQILVYQVPMPEPLFRLEPRATECAKLHAH
ADYGLMRVKLYEDIVHHGSIATTYDYPVIVNGRYLSSPSPIPKFDNPKLHMNPALQLFGAGRERRIYAIAPYTPVASLDF
DDHPFEVQRWDAACALCGSRESFLDEMIVDDHGTRRFVCSDSDYCSDRRGGAQARADGGDEGNGGGDGEMPAPLAAEQPL
GETT
>Mature_324_residues
MNAPDHCAAAAPDTHAAEGYNFAYLDEQTKRMTRRALLKAVAVPGYQVPFASREMPLPFGWGTGGIQVTAALIGRDDTLK
VIDQGSDETTNAVNIRRFFARTAGVATTTKTAQATIIQTRHRVPETPLTERQILVYQVPMPEPLFRLEPRATECAKLHAH
ADYGLMRVKLYEDIVHHGSIATTYDYPVIVNGRYLSSPSPIPKFDNPKLHMNPALQLFGAGRERRIYAIAPYTPVASLDF
DDHPFEVQRWDAACALCGSRESFLDEMIVDDHGTRRFVCSDSDYCSDRRGGAQARADGGDEGNGGGDGEMPAPLAAEQPL
GETT

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=267, Percent_Identity=70.7865168539326, Blast_Score=416, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 35537; Mature: 35537

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDHCAAAAPDTHAAEGYNFAYLDEQTKRMTRRALLKAVAVPGYQVPFASREMPLPFG
CCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WGTGGIQVTAALIGRDDTLKVIDQGSDETTNAVNIRRFFARTAGVATTTKTAQATIIQTR
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHH
HRVPETPLTERQILVYQVPMPEPLFRLEPRATECAKLHAHADYGLMRVKLYEDIVHHGSI
HCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCEEEEHHHHHHHHCCCE
ATTYDYPVIVNGRYLSSPSPIPKFDNPKLHMNPALQLFGAGRERRIYAIAPYTPVASLDF
EEEECCEEEECCEEECCCCCCCCCCCCEEEECCHHHEECCCCCCEEEEEECCCCEEECCC
DDHPFEVQRWDAACALCGSRESFLDEMIVDDHGTRRFVCSDSDYCSDRRGGAQARADGGD
CCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
EGNGGGDGEMPAPLAAEQPLGETT
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPDHCAAAAPDTHAAEGYNFAYLDEQTKRMTRRALLKAVAVPGYQVPFASREMPLPFG
CCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WGTGGIQVTAALIGRDDTLKVIDQGSDETTNAVNIRRFFARTAGVATTTKTAQATIIQTR
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHH
HRVPETPLTERQILVYQVPMPEPLFRLEPRATECAKLHAHADYGLMRVKLYEDIVHHGSI
HCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCEEEEHHHHHHHHCCCE
ATTYDYPVIVNGRYLSSPSPIPKFDNPKLHMNPALQLFGAGRERRIYAIAPYTPVASLDF
EEEECCEEEECCEEECCCCCCCCCCCCEEEECCHHHEECCCCCCEEEEEECCCCEEECCC
DDHPFEVQRWDAACALCGSRESFLDEMIVDDHGTRRFVCSDSDYCSDRRGGAQARADGGD
CCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
EGNGGGDGEMPAPLAAEQPLGETT
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]