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The map label for this gene is potI [H]

Identifier: 126455120

GI number: 126455120

Start: 2161076

End: 2161894

Strand: Reverse

Name: potI [H]

Synonym: BURPS1106A_2175

Alternate gene names: 126455120

Gene position: 2161894-2161076 (Counterclockwise)

Preceding gene: 126452007

Following gene: 126452610

Centisome position: 54.2

GC content: 64.35

Gene sequence:

>819_bases
ATGAAGCCGAATCGCTATCTGCAGGTGCTGATGCTCGCGATCGGCTTCGGTTTCCTCTATATCCCGATCGCGAGCCTCGT
CGTCTATTCGTTCAACGATTCGAAGCTCGTCACCGTGTGGTCCGGTTTCTCGACGCGCTGGTACGCGGCACTCGTCGAGG
ACGACGAGCTGATCGCGGCCGCGTGGCTGTCGCTGAAGATCGCGGTGATGACCGCGTTCGCATCGGTGTTCATCGGCACG
TGGGCGGGTTTCGTGCTCGCGCGGATGGGCCGCTTTCGCGGCTTCGCGCTCTACAGCGGGATGATCAACGCGCCGCTCGT
GATTCCCGAGGTGATCCAGGGCATCTCGCTGCTGCTGCTGTTCATCGAGCTCGGCAAGTGGTTCGGATGGCCCGCCGGCC
GTGGGATGTTCACGATCTGGCTCGGCCACGTGATGCTGTGCATCTCGTACGTCGCGATCATCGTGCAGTCGCGCGTGCGC
GAGCTGAACCCGTCGCTCGAGGAGGCGGCGCTCGATCTCGGCGCGACCCCGTTCAGGGTGTTCTTCTCGATCACGCTGCC
GCTGATCTCCCAGGCGCTCGTGTCCGGCTGGCTGCTGTCGTTTACGCTGTCGATCGACGATCTGGTGCTGTCGGCGTTCC
TGTCCGGACCCGGCTCGACGACGCTGCCGCTCGTCGTGTTCTCGCGCGTGCGCCTCGGCCTGAATCCGGAGATGAACGCG
CTCGCGACGCTGTTCATCGTCGCGGTGACGATCGGCGTCGTGGCGGCGAACTATGTGATGCTGCGTCAGGAGAAGAGGCG
GATGGCCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CGATGGCGTCGGCGGTGACCTGCGCGATGGTGCTGCTGCTCCTCGTGCCGATGGCGATGTTCCAGCACTTCCAGGCGAAG
GAGCAGGAGGGCCACCGCCG

Downstream 100 bases:

>100_bases
CGCGGCGGCGGCGCGAGCCGCAAGTGCCGCGTGCGCGGCGCGGGCGGCGCGACGGCTCGGTCGCACCTCGGTCGCGGATG
GCGCATCGCTTGCCGGTGCG

Product: putrescine ABC transporter, permease protein PotI

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNDSKLVTVWSGFSTRWYAALVEDDELIAAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFRVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVAANYVMLRQEKRRMAEAA

Sequences:

>Translated_272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNDSKLVTVWSGFSTRWYAALVEDDELIAAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFRVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVAANYVMLRQEKRRMAEAA
>Mature_272_residues
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNDSKLVTVWSGFSTRWYAALVEDDELIAAAWLSLKIAVMTAFASVFIGT
WAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPFRVFFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVVAANYVMLRQEKRRMAEAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=263, Percent_Identity=58.5551330798479, Blast_Score=286, Evalue=9e-79,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=38.2716049382716, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787714, Length=245, Percent_Identity=31.0204081632653, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788764, Length=233, Percent_Identity=29.6137339055794, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29927; Mature: 29927

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNDSKLVTVWSGFSTRWYAALVEDDELIAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCHHEEEEECCCHHHHH
AWLSLKIAVMTAFASVFIGTWAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPFRV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVVAANYVMLRQEKRRMAEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPNRYLQVLMLAIGFGFLYIPIASLVVYSFNDSKLVTVWSGFSTRWYAALVEDDELIAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCHHEEEEECCCHHHHH
AWLSLKIAVMTAFASVFIGTWAGFVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELGKWFGWPAGRGMFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPFRV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVVAANYVMLRQEKRRMAEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]