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The map label for this gene is priA [H]

Identifier: 126455110

GI number: 126455110

Start: 3939997

End: 3942264

Strand: Reverse

Name: priA [H]

Synonym: BURPS1106A_4035

Alternate gene names: 126455110

Gene position: 3942264-3939997 (Counterclockwise)

Preceding gene: 126451836

Following gene: 126454416

Centisome position: 98.84

GC content: 72.4

Gene sequence:

>2268_bases
ATGGCTGATGCGTTCGTGCGCGTCGCGCTCGATCATCCGCTGCCGACGCTGTTCGATTACCGGTATCGCGGCGACATGCC
GGCGGCGCCCGGGATGCTCGTGCAGGTGCCGTTCGGCCGGCGCAGCGTGGTCGGGCTCGTATGCGAAGTGACCGCTCACA
CGGACGTGCCGGCGGCCAAGCTGAAGGCCGTCGATGCGGTCTGTACGGAGCTGCCGCCGCTGTCGCGGCACTGGCTCGAA
CTCGCCGCGTTCGCGGCCGACTACTATCAGCGCGGGCTCGGTGAAGTTGCGCTGCCGGCGCTGCCGCAGGCGCTGCGCGA
CGTGCCGCGCTGGGGGCGGCTGCTTGCGCCCGAGCTGCGCTACCGGCTGCTCGACGCCGGGCGCGCCGCGCTGCCGGACG
CGCTGCCCGCGCGCGGCGCGGCGCTGCGCCGGCTCGCGCAGGCGCTCGCCGACGCGCCTGCGCTCTCGCTCGCCGAGGTT
CGCGCGCTGCATCCGAAGGCCGTCGCGGTGCTCGACGAATGGCAGGCGCAAGGCTGGGCCGCGTGCGACGAGATTGCGAT
CACGCACGCCGATGCGCAGCTTGTGGATAACCCGGCACCGGCGTCCGCTCGGCGCCCGACTGCATTGCCGCCGCAACTGA
CCGATCAACAGGCGGAGGCGCTCGACGCGATCGCGGCGGCGGACGGTTTCGCGCCATTCCTGCTGCACGGCGTGACGGGC
AGCGGCAAGACCGAGGTGTATCTGCGCGCGCTCGCCGCGCTGCTCGAGCGGCGGCCCGAAGCACAGGCGCTCGTCCTCGT
GCCCGAGATCAACCTGACGCCGCAGTTCGAAGCGGCGTTTCGCGCGCGCTTCGCCGCGACGCTGCCCGCCGATGCGATCG
TCACGCTGCACAGCGGCCTTGCCGAAGGCGAGCGCGCGCGCCACTGGCTCGCCGCACATACCGGCCGCGCGCGGATCGTG
CTCGGCACGCGGCTCGCGGTGCTCGCGTCGCTGCCGGCGCTTGCGATGATCGTCGTCGACGAGGAGCACGAGCCGGCCTA
CAAGCAACAGGAAGGGTTGCGCTATTCGGCGCGCGATCTCGCCGTGTGGCGGGCGAAGCAGCTCGCGATTCCGGTCGTCC
TCGGCTCGGCGACACCGTCGCTCGAAACCTGGTGGCAGGCCGAGCAGGGCCGCTACCGGCGGCTCACGCTGACGAAGCGC
GCGGTGGCCGACGCGGCGCTGCCGACGGTGCGGCTCGTCGATCTCGAGGAGGAGCGGCGGCGCGGGCGCGGGTCGATGGG
CGGGCTGTCGGGGCCGCTCGTCGCTGCGCTGAAGGCGCGGCTCGAGCGTGGCGAGCAGAGCCTCGTGTTCCTGAACCGGC
GCGGCTATGCCCCCGCGCTCGCGTGCGACGCGTGCGGCTGGGTCGCAGGCTGCCCGCGATGCAGCGCGTATGTCGTGATG
CACAAGCCCGAGCACGCGGTGCGCTGCCATCACTGCGGCTGGGAGGCGCGCATTCCGCGCGCGTGCCCGGAATGCGGGAA
CGTCGATATCGCGCCGCTCGGCCGCGGCACGCAGCGCGTCGAGGAGACGCTCGCGCTCGCCGTGCCGGGCGCGCGGATCC
TGCGCATCGACGCGGACAGCACGCGCCGCAAGGGCAGCGCGCAGGCGCTCTTCTCGGACGTGCACGCGGGCGAGGTCGAC
ATCCTCGTCGGCACGCAGATGATCGCGAAGGGGCACGATTTCCAGCGGGTGTCGCTCGTCGGCGTGCTGAATGCGGATAC
CGCATTGTTCTCGCACGATTTTCGCGCAAGCGAGCGGCTATTCGCGCAACTGATGCAGGTGAGCGGCCGCGCGGGGCGCG
CGGGACTGCCGGGCGAGGTGCTGATCCAGACGCGATATCCGCGTCACGCGCTCTATCACGCGCTCGCGCGCCAGGATTAC
GTGGGGTTCGCCGGTGCGACGCTCGGCGAGCGGCGCGACGCGCACCTGCCGCCGTTCGTCTATCAGGCGCTCTTGCGCGC
CGAGGGGCGCACGCTCGATGCGGCGCTCGCCTTCCTGCAGCAGGCGGCCGACGCGTTCTCGGGGCTGCCGGGCGCGGAGC
GCGTGACGGTGTACGACGCCGTGCCGATGACGATCGTCAAGGTGGCCAATGTCCACCGGGCGCAACTGTTGATCGAGAGC
GCGTCGCGCGCGGCGCTTCAGCACACGCTGCGCGCGTGGCAGCCGCTGCTGCGCGAGCTGAAGGGCGTGCTGCGCTGGAG
CGTCGAGGTCGATCCGCTCGACATCTGA

Upstream 100 bases:

>100_bases
ATCCAAAACTTAGCGCCGAAAGTGACGTTTCACTTTAACTTCACGGGCGTCGCGGATATCCGGCCTGCCGGGCCTGTCGG
CATTCGGCGGTGCCGCACCG

Downstream 100 bases:

>100_bases
CGCCGGCGCGTCATTCGTGCGCTTCGCGCAGGCCGGACAGCCGCGCGCGGCGCTCGTCCTTCTTCACGCGTTGCGCACAT
CCGAGCGCCTGGAAAAACAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Sequences:

>Translated_755_residues
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLE
LAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEV
RALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIV
LGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKR
AVADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVD
ILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDY
VGFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
>Mature_754_residues
ADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAKLKAVDAVCTELPPLSRHWLEL
AAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELRYRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVR
ALHPKAVAVLDEWQAQGWAACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTGS
GKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAAHTGRARIVL
GTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRA
VADAALPTVRLVDLEEERRRGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVMH
KPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDI
LVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYV
GFAGATLGERRDAHLPPFVYQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIESA
SRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=41.501976284585, Blast_Score=490, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81882; Mature: 81751

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
ADAFVRVALDHPLPTLFDYRYRGDMPAAPGMLVQVPFGRRSVVGLVCEVTAHTDVPAAK
CCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHH
LKAVDAVCTELPPLSRHWLELAAFAADYYQRGLGEVALPALPQALRDVPRWGRLLAPELR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCHHHH
YRLLDAGRAALPDALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVLDEWQAQGWA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
ACDEIAITHADAQLVDNPAPASARRPTALPPQLTDQQAEALDAIAAADGFAPFLLHGVTG
CHHHHEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCC
SGKTEVYLRALAALLERRPEAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGL
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
AEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDL
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCHHHH
AVWRAKQLAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVRLVDLEEERR
HHHHHHHEEEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEEECCHHHHH
RGRGSMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCPRCSAYVVM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCCCCCCEEEEE
HKPEHAVRCHHCGWEARIPRACPECGNVDIAPLGRGTQRVEETLALAVPGARILRIDADS
ECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
TRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERL
CCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHH
FAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGERRDAHLPPFV
HHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YQALLRAEGRTLDAALAFLQQAADAFSGLPGAERVTVYDAVPMTIVKVANVHRAQLLIES
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
ASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]