Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 126454794

GI number: 126454794

Start: 3281938

End: 3283356

Strand: Direct

Name: tgt [H]

Synonym: BURPS1106A_3361

Alternate gene names: 126454794

Gene position: 3281938-3283356 (Clockwise)

Preceding gene: 126455279

Following gene: 126451936

Centisome position: 82.29

GC content: 66.53

Gene sequence:

>1419_bases
TTGGCCCATCGGCCGAACAGCCGATCAGCCGATCGTTCGATTGGCTGTTCGGCTGTTCGGCTGTTCGGCGGCTCGGCGGC
TCGGCGGCTCGGCGGCAAGGTAACGACGATCGCGCGCCGAACCGCCGCGAGCCGCGCTTCACTTTTTTCCTTCGCGGCAA
GCCGACGGCTCGCCGCGCCTTCAACATGCGCCGGACTGTATTCCGGTGGCAAGGAGTCCAACCAGATGACGACCGAAGGT
CCATCGCACGATACGCACGATACCTCCGCGGGCATTCGCCCGCACGGCGGCCTGAAATTCGAACTGCTGACGACCGACGG
CCGCGCACGGCGCGGCCGCGTGACGCTCAATCACGGCGTCGTCGAGACGCCGATCTTCATGCCGGTCGGCACGTACGGCA
CCGTGAAGGCCGTGCAGCCGCGCGAACTCGACGAAATGCGCGCGCAGATCATCCTCGGCAACACGTTCCACCTGTGGCTG
CGCCCCGGCCTCGAGACGATCGGCGCGCACGGCGGGCTGCACCGCTTCATGGGCTGGAACAAGCCGATCCTCACCGATTC
GGGCGGCTTCCAGGTGTTCTCGCTCGGCGATCTGCGCAAGATCACCGAGGAAGGCGTCACGTTCGCGTCGCCGATCAACG
GCGATCGGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGACGCTGAATTCCGACATCGTGATGCAGTTCGAC
GAATGCACGCCGTACGCGACGAACGGCGTGCCGACGACGCACCGGGAAGCGGCCGAATCGATGCGGATGTCGCTGCGCTG
GGCGAAGCGCTCGCTCGACGAATTCGAGCGCCTCGGCAACCCGAACGCGCTGTTCGGCATCGTGCAGGGCGGGATGTACG
AGGACCTGCGCGACGAATCGCTCGCCGGGCTCTCGGCGCTCGGCTTCCACGGCCTCGCGATCGGCGGGCTGTCGGTCGGC
GAGCCGAAGGAGGACATGATGCGCGTGCTCGAGCACGTCGCGCCGCGCCTGCCCGCCGACAAGCCGCACTACCTGATGGG
CGTGGGCACGCCGGAGGATCTCGTCGCGGGTGTCGCGGCGGGCGTCGACATGTTCGACTGCGTGATGCCGACCCGCAACG
CGCGCAACGGCTGGCTCTTCACGCGCCTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAATTCGCTCAAGCCGCTC
GACGAGACCTGCGGCTGCTATACGTGCCGGAATTTCTCGCGCGGCTACCTGCATCATCTGCACCGCGTCGGCGAGATTCT
CGGCGCGCAGTTGAACACGATCCACAACCTGCACTACTACCTCGATCTGATGCGCGAGATCCGCGAGGCGATCGAGACGC
ACACGTTCGACGCGTTCCAAAAGCGCTTCGCGCAGGACCGCGCGCGCGGCGTCGACTGA

Upstream 100 bases:

>100_bases
TACCGGTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGGCGCGACGACGCGGCCGAGGCGACGCACGGCGGCGCATA
AGCGCGGCGATTCGCGCCGA

Downstream 100 bases:

>100_bases
CGCCGCGCGCCGGCATCGGCATCGGCATTGGCATTGGCGGCGCCGCGCTCGCGCGCCAATCGCGCGGGCAATGCCGCCGC
GCTTCGCAGCCCAATCGTCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAHRPNSRSADRSIGCSAVRLFGGSAARRLGGKVTTIARRTAASRASLFSFAASRRLAAPSTCAGLYSGGKESNQMTTEG
PSHDTHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLWL
RPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFD
ECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLSVG
EPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRLGDVKIRNATHKNSLKPL
DETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFDAFQKRFAQDRARGVD

Sequences:

>Translated_472_residues
MAHRPNSRSADRSIGCSAVRLFGGSAARRLGGKVTTIARRTAASRASLFSFAASRRLAAPSTCAGLYSGGKESNQMTTEG
PSHDTHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLWL
RPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFD
ECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLSVG
EPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRLGDVKIRNATHKNSLKPL
DETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFDAFQKRFAQDRARGVD
>Mature_471_residues
AHRPNSRSADRSIGCSAVRLFGGSAARRLGGKVTTIARRTAASRASLFSFAASRRLAAPSTCAGLYSGGKESNQMTTEGP
SHDTHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLWLR
PGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFDE
CTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLSVGE
PKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRLGDVKIRNATHKNSLKPLD
ETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFDAFQKRFAQDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=44.6629213483146, Blast_Score=298, Evalue=6e-81,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=66.3101604278075, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=43.1266846361186, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=29.7101449275362, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=45.3781512605042, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21355311, Length=166, Percent_Identity=28.9156626506024, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 51916; Mature: 51785

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRPNSRSADRSIGCSAVRLFGGSAARRLGGKVTTIARRTAASRASLFSFAASRRLAAP
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
STCAGLYSGGKESNQMTTEGPSHDTHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCE
VETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWN
EECCEEEECCCCCCEECCCCCCHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCC
KPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFD
CCEEECCCCEEEEECCHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEEC
ECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDES
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
LAGLSALGFHGLAIGGLSVGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAA
HHCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHH
GVDMFDCVMPTRNARNGWLFTRLGDVKIRNATHKNSLKPLDETCGCYTCRNFSRGYLHHL
CCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHCCCHHHCCCCHHHHHHH
HRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFDAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHRPNSRSADRSIGCSAVRLFGGSAARRLGGKVTTIARRTAASRASLFSFAASRRLAAP
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
STCAGLYSGGKESNQMTTEGPSHDTHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCE
VETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWN
EECCEEEECCCCCCEECCCCCCHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCC
KPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFD
CCEEECCCCEEEEECCHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEEC
ECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDES
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
LAGLSALGFHGLAIGGLSVGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAA
HHCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHH
GVDMFDCVMPTRNARNGWLFTRLGDVKIRNATHKNSLKPLDETCGCYTCRNFSRGYLHHL
CCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHCCCHHHCCCCHHHHHHH
HRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFDAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA