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The map label for this gene is purN [H]

Identifier: 126454710

GI number: 126454710

Start: 956322

End: 956984

Strand: Direct

Name: purN [H]

Synonym: BURPS1106A_0973

Alternate gene names: 126454710

Gene position: 956322-956984 (Clockwise)

Preceding gene: 126454774

Following gene: 126454034

Centisome position: 23.98

GC content: 69.53

Gene sequence:

>663_bases
ATGAAAAAACTTGTCATCCTGATTTCCGGCCGCGGCAGCAACATGGAGGCCATCGTACGCGCCTGCGCGCGCGAAGGCTG
GCCCGCCGAGGTCGCCGCCGTGATTTCCAACCGGCCGGGCGCGGCCGGCCTCGAATTCGCGGCGTCGCACGGCATCGCGA
CGGCCGTCGTCGACCACCGCGCGTTCGACGGCCGCGACAGCTTCGATGCGGCGCTCGCCGCCGAAATCGACCGCTTCGCC
CCCGATCTCGTCGTGCTCGCCGGCTTCATGCGCATTCTCACGCCGGCGTTCGTCGCCAAATATGAGGGGCGGATGCTCAA
CATCCATCCGTCGCTGCTGCCGAGCTTCAAGGGCATTCATACGCATCAGCAGGCGCTCGACGCGGGCGTCGCGCTGCACG
GCGCATCCGTCCACTTCGTGATTCCGGAGCTCGACAGCGGCGCGATCGTCGCGCAGGCGGCCGTGCCCGTCGTCGCGGGC
GACGATGCCGATGCGCTCGCCGCGCGCGTGCTCGCCGCCGAGCACACGCTGTATCCGCGCGCGGTGCGCTGGTTCGTCGA
AGGCAAGCTGCGCCTCGACGCGGGGCGCGCCATCGTTGCGCCCGACGAGGCGCGCTGGCTATTCGCGGATGCGATCGACA
CGTCGACGAGCGAGGGCGTATGA

Upstream 100 bases:

>100_bases
AAGCGCGGCCGGATGCCGCAAAGCAAGAGATTATAAACGTTCGGGCGGCCGGGCGGCTCAGGATGCCGGAAACGGCGGGG
CCGAATGGTAAAATCCGCGG

Downstream 100 bases:

>100_bases
AGCTGCACGGTTTCCTGATTGGCCAGACCGAGGCGCTCCTCGCCGAAGTCCTGAAATTCGCCGGCCCGGCCGACGTGACG
ACGAGCCGCTTCTTCCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHRAFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DDADALAARVLAAEHTLYPRAVRWFVEGKLRLDAGRAIVAPDEARWLFADAIDTSTSEGV

Sequences:

>Translated_220_residues
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHRAFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DDADALAARVLAAEHTLYPRAVRWFVEGKLRLDAGRAIVAPDEARWLFADAIDTSTSEGV
>Mature_220_residues
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHRAFDGRDSFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAG
DDADALAARVLAAEHTLYPRAVRWFVEGKLRLDAGRAIVAPDEARWLFADAIDTSTSEGV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=3e-45,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=45.2631578947368, Blast_Score=178, Evalue=3e-45,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=54.2105263157895, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=30.8080808080808, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=41.6666666666667, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=30.1507537688442, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.973544973545, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23222; Mature: 23222

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHCCEEEEEECCC
AFDGRDSFDAALAAEIDRFAPDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAGDDADALAARVLAAEHTLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEEECEEEEECCCHHHHHHHHHHHHCCCCHH
AVRWFVEGKLRLDAGRAIVAPDEARWLFADAIDTSTSEGV
HHHHEECCEEEEECCCEEECCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKLVILISGRGSNMEAIVRACAREGWPAEVAAVISNRPGAAGLEFAASHGIATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHCCEEEEEECCC
AFDGRDSFDAALAAEIDRFAPDLVVLAGFMRILTPAFVAKYEGRMLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQAAVPVVAGDDADALAARVLAAEHTLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEEECEEEEECCCHHHHHHHHHHHHCCCCHH
AVRWFVEGKLRLDAGRAIVAPDEARWLFADAIDTSTSEGV
HHHHEECCEEEEECCCEEECCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]