Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 126454697

GI number: 126454697

Start: 2227343

End: 2228311

Strand: Reverse

Name: pcm [H]

Synonym: BURPS1106A_2238

Alternate gene names: 126454697

Gene position: 2228311-2227343 (Counterclockwise)

Preceding gene: 126451735

Following gene: 126454413

Centisome position: 55.87

GC content: 73.27

Gene sequence:

>969_bases
ATGAGCGGCGAGCGCGCGAAGCGGTTTCCGCTCGCGCTCGAGGATCTGAAGCGCGAGCCGCGCAAGCCCGAGGGCCGCGT
GGCGGAGCGCCAGGCGGCGGGAGACGCCGCCCGCCAGCGCCTCACCGCGGCGGCCGCCGTGCCCGCCGCTGCTTCTCCGA
TCGTGCCCGAACGCCGCGCGCCGCACGGCGGCGTGTTCGCCGCGAAACCCGCGCGTGCGAAGCAGCACGCGCCTGCGGCG
CCCGGCGCCGCGAAGCGCGCGCCGCAGGGGGGCGCGAAGCAGGGGGACCGGAGCGCCGCGCCGAACGTCGCGCTGAGCGG
CGCGCTCGCGCTGACTTCGGAACGGGTGCGCGAGCGCATGGTCGAACGGCTGCGGGCAAACGGCGTGGCCGATCCGCGCG
TGCTCGCCGCGATGTCGGCCGTGCCGCGCCACATGTTCGTCGATCCCGGGCTCGCGGCTCAGGCGTACGAGGACGCCGCG
CTGCCGATCGGCCATCAGCAGACGATCTCGAAGCCGTCGGTCGTCGCGCGCATGATCGAGCTCGCGGCGGCGGGCCGCGC
GCTCGAGCGCGTGCTCGAAATCGGCACGGGCTGCGGCTATCAGGCCGCGGTGTTGAGCCGCGTCGCGCGCGACGTGTATT
CGATCGAGCGCGTGAAGCCGCTCTACGAGCGCGCAAAACTGAATTTGCGGCCGCTGCGCGTGCCGAACATCCGCCTGCAC
TACGGCGACGGACGCGTGGGCCTGCCCGCCGCGGCGCCGTTCGACGCGATCGTGATCGCGGCGGCGGGGCTCGACGTGCC
GCGGGCGCTGTTGGAACAATTGGCGATCGGCGGCAGGCTCGTCGCGCCTGTCGGAGAGCAGGCGGGCGAGCAGGTGCTGA
CGCTCGTGGAGCGCGTCGCACCCGCGCAGTGGCGCGAGTCGCGGCTTGATCGCGTTTTCTTTGTCCCTTTAAAATCCGGA
GTGATTTGA

Upstream 100 bases:

>100_bases
CGTCGGGCTACGTGTCGATCACGCCGCTGCAGCTCGATCTCACGCATACGCAGATGCTCGCAGCCACGCGCGATTGGGCG
CGTGCCGGGAGCGGCGCTTC

Downstream 100 bases:

>100_bases
TACCGATGAGTATGCTGCGCGCGATGCAAAACAATCGTTCGAAGGTTCCGCTCTCGCTCGCTCAGCGCGCGATTTGCATG
GCGGCGTTCGCGCTGCTCGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSGERAKRFPLALEDLKREPRKPEGRVAERQAAGDAARQRLTAAAAVPAAASPIVPERRAPHGGVFAAKPARAKQHAPAA
PGAAKRAPQGGAKQGDRSAAPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAA
LPIGHQQTISKPSVVARMIELAAAGRALERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPNIRLH
YGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFVPLKSG
VI

Sequences:

>Translated_322_residues
MSGERAKRFPLALEDLKREPRKPEGRVAERQAAGDAARQRLTAAAAVPAAASPIVPERRAPHGGVFAAKPARAKQHAPAA
PGAAKRAPQGGAKQGDRSAAPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAA
LPIGHQQTISKPSVVARMIELAAAGRALERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPNIRLH
YGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFVPLKSG
VI
>Mature_321_residues
SGERAKRFPLALEDLKREPRKPEGRVAERQAAGDAARQRLTAAAAVPAAASPIVPERRAPHGGVFAAKPARAKQHAPAAP
GAAKRAPQGGAKQGDRSAAPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAAL
PIGHQQTISKPSVVARMIELAAAGRALERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPNIRLHY
GDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFVPLKSGV
I

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=211, Percent_Identity=31.7535545023697, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789100, Length=210, Percent_Identity=44.7619047619048, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=223, Percent_Identity=33.1838565022422, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=218, Percent_Identity=32.5688073394495, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17981723, Length=218, Percent_Identity=33.0275229357798, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 34158; Mature: 34027

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGERAKRFPLALEDLKREPRKPEGRVAERQAAGDAARQRLTAAAAVPAAASPIVPERRA
CCCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHGGVFAAKPARAKQHAPAAPGAAKRAPQGGAKQGDRSAAPNVALSGALALTSERVRERM
CCCCEEECCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAALPIGHQQTISKPSVVARMIE
HHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
LAAAGRALERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPNIRLH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEE
YGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVA
ECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHC
PAQWRESRLDRVFFVPLKSGVI
CHHHHHHHCCEEEEEECCCCCC
>Mature Secondary Structure 
SGERAKRFPLALEDLKREPRKPEGRVAERQAAGDAARQRLTAAAAVPAAASPIVPERRA
CCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHGGVFAAKPARAKQHAPAAPGAAKRAPQGGAKQGDRSAAPNVALSGALALTSERVRERM
CCCCEEECCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAALPIGHQQTISKPSVVARMIE
HHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
LAAAGRALERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPNIRLH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEE
YGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVA
ECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHC
PAQWRESRLDRVFFVPLKSGVI
CHHHHHHHCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA