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The map label for this gene is purK [H]

Identifier: 126454636

GI number: 126454636

Start: 824525

End: 825721

Strand: Direct

Name: purK [H]

Synonym: BURPS1106A_0844

Alternate gene names: 126454636

Gene position: 824525-825721 (Clockwise)

Preceding gene: 126454425

Following gene: 126453889

Centisome position: 20.67

GC content: 71.6

Gene sequence:

>1197_bases
ATGACTGCACTCCCCACCCCGAATTCCCCGATCCTGCCGGGCGCCTGGCTCGGCATGGTCGGCGGCGGCCAGCTCGGCCG
CATGTTCTGCTTTGCCGCGCAAGCGATGGGCTACCGCGTCGCCGTGCTCGATCCCGATCCGACGAGCCCCGCGGGCGCCG
TCGCCGACAAGCATCTGCGCGCCGCGTACGACGACGAGGCCGCGCTCGCCGAGCTCGCGCAATTGTGCGATGCCGTATCG
ACCGAATTCGAGAACGTGCCCGCCGCGAGCCTCGAGCTGCTCGCGCAATCGACGTTCGTCGCGCCGGCCGGCCGGTGCGT
CGCGATCGCGCAGGACCGGATCGCCGAGAAACGATTCATCGCGGCGTCGGGCGTGCCCGTCGCGCCGCACGTCGTGATCG
AATCGCACGCGCAGCTCGCCGCGCTCGCCGATGCGGACCTCGCCGCGGTGCTGCCCGGCATCCTGAAGACCGCGCGTCTC
GGTTACGACGGCAAGGGGCAGGTGCGTGTCGCGACGGTGCGCGAGGCGCGCGACGCGTACGCGTCGCTCGGCGGCGTGCC
TTGCGTGCTCGAGAAGCGCCTGCCGCTCGAATACGAGGTGTCGGCGCTGATCGCGCGCGGCGCGAACGGCGCGTCGGCGG
TGTTTCCGCTCGCGCAGAACACGCACCACGGCGGCATCCTGTCGCTGAGCGTCGTGCCTGCGCCCGCCGCGAGCGATGCG
CTCGTGCGCGAAGCGCAGCAGGCGGCCGTGCGGATCGCCGATTCGCTCGGCTACGTCGGCGTGCTGTGCGTCGAGTTCTT
CGTGCTCGAAGACGGCTCGCTCGTCGCGAACGAAATGGCGCCGCGCCCGCACAACTCCGGCCATTACACGGTCGACGCGT
GCGAGACGAGCCAGTTCGAGCAGCAGGTGCGCGCGATGACGCGGCTGCCGCTCGGCAGCACGCGCCAGCATTCGCCCGCC
GCGATGCTCAACGTGCTCGGCGACGTGTGGTTCGCGAACGGCGTGTCGGGTGAACCCGTCACGCCGCCGTGGGACGAGGT
CGCCGCGATGCCGACCGCGCGGCTGCATCTGTACGGCAAGGAAGAGGCGCGCGCCGGCCGCAAGATGGGCCATGTGAACT
TCACCGCGGCGACGCGCGACGAAGCGGTCGCCGGCGCGACCGCGTGCGCGCGGCTGTTGCGCATTGCGCTCGACTGA

Upstream 100 bases:

>100_bases
ATGGCGCTGCCGCCGCTGTGAGGCCGCCGCGCGAGCCGCTTGCAACATCGATTGTCCTGACCGCGGCGGGCGCCGCCGCC
CGCCGCCACCGACCCTAGAC

Downstream 100 bases:

>100_bases
CGGAGCGCGCGATGTCGAACGATCGACCCTTCCCGAGCGCCGAGGCCATCGAGGACGCGGCCGCGCGGCTCGATGCGGGC
GAGCTCGTCGCGTTTCCGAC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTALPTPNSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLDPDPTSPAGAVADKHLRAAYDDEAALAELAQLCDAVS
TEFENVPAASLELLAQSTFVAPAGRCVAIAQDRIAEKRFIAASGVPVAPHVVIESHAQLAALADADLAAVLPGILKTARL
GYDGKGQVRVATVREARDAYASLGGVPCVLEKRLPLEYEVSALIARGANGASAVFPLAQNTHHGGILSLSVVPAPAASDA
LVREAQQAAVRIADSLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACETSQFEQQVRAMTRLPLGSTRQHSPA
AMLNVLGDVWFANGVSGEPVTPPWDEVAAMPTARLHLYGKEEARAGRKMGHVNFTAATRDEAVAGATACARLLRIALD

Sequences:

>Translated_398_residues
MTALPTPNSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLDPDPTSPAGAVADKHLRAAYDDEAALAELAQLCDAVS
TEFENVPAASLELLAQSTFVAPAGRCVAIAQDRIAEKRFIAASGVPVAPHVVIESHAQLAALADADLAAVLPGILKTARL
GYDGKGQVRVATVREARDAYASLGGVPCVLEKRLPLEYEVSALIARGANGASAVFPLAQNTHHGGILSLSVVPAPAASDA
LVREAQQAAVRIADSLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACETSQFEQQVRAMTRLPLGSTRQHSPA
AMLNVLGDVWFANGVSGEPVTPPWDEVAAMPTARLHLYGKEEARAGRKMGHVNFTAATRDEAVAGATACARLLRIALD
>Mature_397_residues
TALPTPNSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLDPDPTSPAGAVADKHLRAAYDDEAALAELAQLCDAVST
EFENVPAASLELLAQSTFVAPAGRCVAIAQDRIAEKRFIAASGVPVAPHVVIESHAQLAALADADLAAVLPGILKTARLG
YDGKGQVRVATVREARDAYASLGGVPCVLEKRLPLEYEVSALIARGANGASAVFPLAQNTHHGGILSLSVVPAPAASDAL
VREAQQAAVRIADSLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACETSQFEQQVRAMTRLPLGSTRQHSPAA
MLNVLGDVWFANGVSGEPVTPPWDEVAAMPTARLHLYGKEEARAGRKMGHVNFTAATRDEAVAGATACARLLRIALD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=379, Percent_Identity=32.4538258575198, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788155, Length=388, Percent_Identity=25.2577319587629, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=376, Percent_Identity=32.4468085106383, Blast_Score=195, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41597; Mature: 41466

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPTPNSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLDPDPTSPAGAVADKHLR
CCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALAELAQLCDAVSTEFENVPAASLELLAQSTFVAPAGRCVAIAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHEE
AASGVPVAPHVVIESHAQLAALADADLAAVLPGILKTARLGYDGKGQVRVATVREARDAY
EECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
ASLGGVPCVLEKRLPLEYEVSALIARGANGASAVFPLAQNTHHGGILSLSVVPAPAASDA
HHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHH
LVREAQQAAVRIADSLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACETSQFE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEECCCHHHHH
QQVRAMTRLPLGSTRQHSPAAMLNVLGDVWFANGVSGEPVTPPWDEVAAMPTARLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEEECC
EEARAGRKMGHVNFTAATRDEAVAGATACARLLRIALD
HHHHHCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TALPTPNSPILPGAWLGMVGGGQLGRMFCFAAQAMGYRVAVLDPDPTSPAGAVADKHLR
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALAELAQLCDAVSTEFENVPAASLELLAQSTFVAPAGRCVAIAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHEE
AASGVPVAPHVVIESHAQLAALADADLAAVLPGILKTARLGYDGKGQVRVATVREARDAY
EECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
ASLGGVPCVLEKRLPLEYEVSALIARGANGASAVFPLAQNTHHGGILSLSVVPAPAASDA
HHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHH
LVREAQQAAVRIADSLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACETSQFE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEECCCHHHHH
QQVRAMTRLPLGSTRQHSPAAMLNVLGDVWFANGVSGEPVTPPWDEVAAMPTARLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEEECC
EEARAGRKMGHVNFTAATRDEAVAGATACARLLRIALD
HHHHHCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]