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The map label for this gene is flgJ [H]

Identifier: 126454563

GI number: 126454563

Start: 267918

End: 268853

Strand: Direct

Name: flgJ [H]

Synonym: BURPS1106A_0286

Alternate gene names: 126454563

Gene position: 267918-268853 (Clockwise)

Preceding gene: 126451759

Following gene: 126453685

Centisome position: 6.72

GC content: 66.45

Gene sequence:

>936_bases
GTGACGACTTTCACGAAACCGAACGATCTGTCGCAGCGCTTCGCGCTCGACGTTCAGGGGTTCGACGCGCTGCGCTCGAA
GGCCACGGCGGCTGCGCCGCGCGAGGGTGTGAAGATGGTCGCGGGCCAGTTCGACGCGATGTTCACGCAGATGATGCTGA
AGAGCATGCGCGACGCGACGCCTTCGGACGGCCTGCTCGATTCGAGCTCGTCGAAGATGTACACGTCGATGCTCGATCAG
CAGCTCGCGCAGCAGATGTCGTCGAAGGGCATCGGCGTGGCCGACGCGCTCACCAAGCAGTTGCTGCGCAACGCGAACGT
CGCGCCCGACGCGCAGGGCGAAGGCGGCCTCGCGGCGATGAACGCGCTCGCGAAGGCGTATGCGAACTCGAATGGGGCGC
CAGGCAACGGCGCGCTCGCGGGCACGCGCGGCTATTCGGCGGCGAGCGCGCTCACGCCGCCGCTGAAGGGCAACGGCAAC
TCCGCGCAGGCGGATGCGTTCGTCGAGAAGATGGCGCTCGCCGCGCAGGCGGCGAGCGCGACCACCGGCATTCCGGCGCG
CTTCATCGTCGGCCAGGCGGCGCTCGAATCCGGCTGGGGCAAGCGCGAGATCCGCGGCGCGAACGGCGAATCGAGCTACA
ACGTGTTCGGCATCAAGGCGACGAAGGGCTGGACCGGCCGCACGGTATCGGCCGTCACCACCGAATACGTGAACGGCAAG
CCGCACCGCGTGGTCGCGCAGTTCCGCGCATACGACTCGTACGAGCATGCGATGACCGATTACGCGAACCTGCTGAAAAA
CAATCCGCGCTACGCGAGCGTGCTGAACGCGGGCCACAACGCGGAAGGCTTCGCGCATGGAATGCAGAAGGCCGGCTACG
CAACCGATCCGCACTACGCGAAGAAGCTGATTTCGATCATGCAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
GGCGCTCAACTCGCTCGGCGCGACGCCCGCGGATCTGATGTCGATCCTGCAGGCGATGAAGGCGGCGGGCGCGCTTCGCG
CGGACCTGGAGATCATCTAA

Downstream 100 bases:

>100_bases
CGCGAGCCGAGCAACCGTTCGGTACATTTTGGACCGCGCCGGGCCTGGCCTCGCGCGGTTTTAACGTTTGCTGAAAAAAT
AAACTCCGGTTTCGATCTAA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTTFTKPNDLSQRFALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSSSSKMYTSMLDQ
QLAQQMSSKGIGVADALTKQLLRNANVAPDAQGEGGLAAMNALAKAYANSNGAPGNGALAGTRGYSAASALTPPLKGNGN
SAQADAFVEKMALAAQAASATTGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGK
PHRVVAQFRAYDSYEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYAKKLISIMQQIG

Sequences:

>Translated_311_residues
MTTFTKPNDLSQRFALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSSSSKMYTSMLDQ
QLAQQMSSKGIGVADALTKQLLRNANVAPDAQGEGGLAAMNALAKAYANSNGAPGNGALAGTRGYSAASALTPPLKGNGN
SAQADAFVEKMALAAQAASATTGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGK
PHRVVAQFRAYDSYEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYAKKLISIMQQIG
>Mature_310_residues
TTFTKPNDLSQRFALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSSSSKMYTSMLDQQ
LAQQMSSKGIGVADALTKQLLRNANVAPDAQGEGGLAAMNALAKAYANSNGAPGNGALAGTRGYSAASALTPPLKGNGNS
AQADAFVEKMALAAQAASATTGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKP
HRVVAQFRAYDSYEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYAKKLISIMQQIG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=297, Percent_Identity=42.7609427609428, Blast_Score=231, Evalue=6e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 32719; Mature: 32588

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFTKPNDLSQRFALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDAT
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSDGLLDSSSSKMYTSMLDQQLAQQMSSKGIGVADALTKQLLRNANVAPDAQGEGGLAAM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NALAKAYANSNGAPGNGALAGTRGYSAASALTPPLKGNGNSAQADAFVEKMALAAQAASA
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TTGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCC
PHRVVAQFRAYDSYEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
KKLISIMQQIG
HHHHHHHHHHC
>Mature Secondary Structure 
TTFTKPNDLSQRFALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDAT
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSDGLLDSSSSKMYTSMLDQQLAQQMSSKGIGVADALTKQLLRNANVAPDAQGEGGLAAM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NALAKAYANSNGAPGNGALAGTRGYSAASALTPPLKGNGNSAQADAFVEKMALAAQAASA
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TTGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCC
PHRVVAQFRAYDSYEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
KKLISIMQQIG
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]