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The map label for this gene is citA [H]

Identifier: 126454498

GI number: 126454498

Start: 1241290

End: 1242579

Strand: Direct

Name: citA [H]

Synonym: BURPS1106A_1266

Alternate gene names: 126454498

Gene position: 1241290-1242579 (Clockwise)

Preceding gene: 126454185

Following gene: 126452881

Centisome position: 31.12

GC content: 71.09

Gene sequence:

>1290_bases
ATGGCCGGCGTGGACGGCGCGGATGCGGCACGCGCCGACGCCCGTCGCGACGTCGCGGCGGTTGCGCTCGGCAACGCGAT
CGAATTCTTCGACTTCGGCGCCTATGCGACCTTCGCGGTGATGATCGGCCGCACGTTCTTCCCGACGCAGCGCCCGTTCG
TGAGCCTGCTGCTGTCGGTATCCGTGTTCGGGCTCGGCTTCGTCGTGCGGCCGCTCGGCGCGCTCGTGATCGGCGCGTAC
GCGGACCGCGCGGGACGCAAGCCCGCGATGACGCTGACGCTCGCGATGATGGCGGTCGGCACGGGCGCGATCGCCGTGCT
GCCGGGATACGAGACGATCGGCGTCGCGGCGCCGATCCTGCTCGTCGTCACGCGGCTGATCCAGGGGCTCGCGTGGGGCG
GCGAGGCGGGGCCCGCGACGACCTACATTCTCGAGGCCGCGCCGCCCGAGCGGCGCGCCGCGTACGCGTGCTGGCAGGTC
GCGACGCAAGGTTTCGCGGCGGTCGCGGCGGGGCTCGCGGGTTACGCGCTGACGCTCGCGCTGCCCGAGGCCGAGCTGGA
TGCGTGGGGCTGGCGCGTGCCGTTCGCGCTCGGCCTGCTGGTGCTGCCGGTCGGCGTCTACATTCGCCGGCGGTTATCCG
ACACGATCGACCCGCGCCACGCATATTGCACGACGCGCGAGGTCCTGCGCGAGCTGAACGCTCGGCACCGGCGGCCGATC
GCGATCGGCCTGATGATCCTGCTCGGCAGCACGATCACGCAGTACTTCCTCAACTACATGACGACGTTTGCATTGACCGA
GCTGCATTTGCCGGGGGGCGTCGCGATGCTCGCCACGTTCGTGACAGGCGCGGCGCTCGCGGTGGGCGCGCTCGCGGGCG
GCAGCCTGTCGGATCGCGTCGGACGCCGCGCGATCCTGATCTGGCCGCGCGTGCTGCTGTTGCTGCTGCTCTTTCCAGCG
TTGCAACTGATCGTGTCGCGTCCGACGCCCGCCGTGTTCCTCGCCACGCTGACCGTGCTGTCCGGCCTGCACGGCATGAG
CGGCGCGGCGCTCATCGTGCTGATCGCCGAGAGCTTTCCGCAGCGCGTGCGCTCGACGGGATTCTCGATCGTCTACGCCG
TCGCGGTGTCGTTGTTCGGCGGCACCGCACAGAGCATCGTCACCTGGCTCATCGGCACCACCGGCAACCCGATGGCGCCG
AGCGGCTATCTGCTTGTCGCGAACGCGATCTGCATCGCGGCGGGATGGCTTGCCGCCGAGACCCGGCCGGGCGGGCGCGG
CACGCGCTAG

Upstream 100 bases:

>100_bases
AGGGCGCTTCATGAAAGACCCGCTTGAATCCGCTTGCCCGCCGGTGCCGGGATGCGGAGCCGGCGCCGACGGTGCGGCCT
GTGCGGCCGCCATGGCCGGG

Downstream 100 bases:

>100_bases
GGTCGGCGCGCCGGCTTCGAGCGCGGACGGGAATTCGCCGAAGGAGGCCGTGAGGCGGCCGGCTGCCGACGCGGGCACCG
GCCGCGCGCATCGGCGTTCG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAGVDGADAARADARRDVAAVALGNAIEFFDFGAYATFAVMIGRTFFPTQRPFVSLLLSVSVFGLGFVVRPLGALVIGAY
ADRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRAAYACWQV
ATQGFAAVAAGLAGYALTLALPEAELDAWGWRVPFALGLLVLPVGVYIRRRLSDTIDPRHAYCTTREVLRELNARHRRPI
AIGLMILLGSTITQYFLNYMTTFALTELHLPGGVAMLATFVTGAALAVGALAGGSLSDRVGRRAILIWPRVLLLLLLFPA
LQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAP
SGYLLVANAICIAAGWLAAETRPGGRGTR

Sequences:

>Translated_429_residues
MAGVDGADAARADARRDVAAVALGNAIEFFDFGAYATFAVMIGRTFFPTQRPFVSLLLSVSVFGLGFVVRPLGALVIGAY
ADRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRAAYACWQV
ATQGFAAVAAGLAGYALTLALPEAELDAWGWRVPFALGLLVLPVGVYIRRRLSDTIDPRHAYCTTREVLRELNARHRRPI
AIGLMILLGSTITQYFLNYMTTFALTELHLPGGVAMLATFVTGAALAVGALAGGSLSDRVGRRAILIWPRVLLLLLLFPA
LQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAP
SGYLLVANAICIAAGWLAAETRPGGRGTR
>Mature_428_residues
AGVDGADAARADARRDVAAVALGNAIEFFDFGAYATFAVMIGRTFFPTQRPFVSLLLSVSVFGLGFVVRPLGALVIGAYA
DRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRAAYACWQVA
TQGFAAVAAGLAGYALTLALPEAELDAWGWRVPFALGLLVLPVGVYIRRRLSDTIDPRHAYCTTREVLRELNARHRRPIA
IGLMILLGSTITQYFLNYMTTFALTELHLPGGVAMLATFVTGAALAVGALAGGSLSDRVGRRAILIWPRVLLLLLLFPAL
QLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPS
GYLLVANAICIAAGWLAAETRPGGRGTR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=29.5348837209302, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788942, Length=386, Percent_Identity=35.4922279792746, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=32.3040380047506, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=28.3062645011601, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082231, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082404, Length=337, Percent_Identity=24.0356083086053, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44993; Mature: 44861

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVDGADAARADARRDVAAVALGNAIEFFDFGAYATFAVMIGRTFFPTQRPFVSLLLSV
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SVFGLGFVVRPLGALVIGAYADRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LVVTRLIQGLAWGGEAGPATTYILEAAPPERRAAYACWQVATQGFAAVAAGLAGYALTLA
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LPEAELDAWGWRVPFALGLLVLPVGVYIRRRLSDTIDPRHAYCTTREVLRELNARHRRPI
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
AIGLMILLGSTITQYFLNYMTTFALTELHLPGGVAMLATFVTGAALAVGALAGGSLSDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GRRAILIWPRVLLLLLLFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFP
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPSGYLLVANAICIAAGWLAAE
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TRPGGRGTR
CCCCCCCCC
>Mature Secondary Structure 
AGVDGADAARADARRDVAAVALGNAIEFFDFGAYATFAVMIGRTFFPTQRPFVSLLLSV
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SVFGLGFVVRPLGALVIGAYADRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
LVVTRLIQGLAWGGEAGPATTYILEAAPPERRAAYACWQVATQGFAAVAAGLAGYALTLA
HHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LPEAELDAWGWRVPFALGLLVLPVGVYIRRRLSDTIDPRHAYCTTREVLRELNARHRRPI
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
AIGLMILLGSTITQYFLNYMTTFALTELHLPGGVAMLATFVTGAALAVGALAGGSLSDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GRRAILIWPRVLLLLLLFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFP
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPSGYLLVANAICIAAGWLAAE
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TRPGGRGTR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]