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The map label for this gene is gatA [H]

Identifier: 126454185

GI number: 126454185

Start: 1239639

End: 1241033

Strand: Direct

Name: gatA [H]

Synonym: BURPS1106A_1265

Alternate gene names: 126454185

Gene position: 1239639-1241033 (Clockwise)

Preceding gene: 126453306

Following gene: 126454498

Centisome position: 31.08

GC content: 73.48

Gene sequence:

>1395_bases
ATGTCCGAGTTGCTCGACCTGAGCGCTTGCTCGCTTGCCGAGCGGTTCAAGAACCGCAGCGTGACGCCCGTCGATTACGC
GCACGCGCTGCTCGACCACATTGCCCGTTGGGAGCCGCATCTGAATGCGCTGTGCCGCTTCGATCCGGCGCGCGTGCGTG
ACGAGGCGCTCGCGTCGACGCGCCGCTGGGCGGCGGGCGCGCCGCTCAGCGAGATCGACGGCGTGCCGGTGACGATCAAG
GAACTGATCGCGACGCAGGGCGACCGGGTGGCGCAAGGCTCGGCGGCCGCCGCCGACGCGCCGCCCGCGACCGTCGACGC
GCCCGCCGCGGCGCGGCTGCGCGAAGCGGGCGCGGTCGTGATCGGCAAGACGACGGTGCCCGATTACGCGATGCTGTCGT
CGGGGCTGTCGAGCCTGTACGGCATCACGCGCAACCCGTGGCGGCTCGATCTGAATCCGGGCGGATCGAGCAGCGGCGCG
GCGGCGGCCGCCGCCGCGGGCTACGGGCCGCTGCACGTCGGCACCGACATCGGCGGGTCGATCCGGCTGCCGGCCGGCTG
GTGCGGGATCGTCGGCTTCAAGCCGTCGAACGGGCGGATTCCGATCGAGCCGTACTACACGGGCCGCTGCGCGGGGCCGA
TGACGCGCACGATCGACGATGCGGCGCTGCTGATGCGCTTCCTGTCGCGGCCGGACTGGCGCGACGCGACGAGCCTGCCG
CCCGAGACGATCGACTGGTCGATCGCGCCCGCGCACGTCAAGGGCATGCGGATCGGCCTGATGCTCGACGCCGGCTGCGG
ACTCGCGCCGGATCCGCAGATCGTCGCCGCGGTGGAGGCGGCGGCCGAGCGTTTCGCCGCGCACGGCGCGCGGATCGTCG
CCGCGCCGCCCGTGCTGTCGCGGCCGATGCTCGAGGGGCTCGACCGCTTCTGGCGGGCGCGGCTATGGGCGGACCTCGAA
CGGCTGCCGGACGCGGCGCGCGCGCGCATCCTGCCGTACATCGCCGAATGGGCGGGGCAGGGCGCGTCGGTGTCGGGCGT
CGAGGCGGTGCGCGGCTTCGGCGCGACGTTCGAGATGCGCGCGGCCGCCGCACGCCTGTTCCAGTCGGTCGACGCGGTGC
TGTCGCCGACCAACGTGGTCACCGGCTTTCCAGCCGAATGGGCGGGGCCGACCGATGATCCTGCGCGGCCGTTCGAGCAC
ATCGCGTTCACGGTTCCGTGGAACATGGGCGAGCAGCCGGCGCTGTCGATCAACTGCGGCTTCGCGCGCGACGGGATGCC
GATCGGCCTGCAGATCGTCGGGCCGCGTTTTGCCGATCGGCTCGTGCTGCAGCTCGGTCGCGCGTACGAGGCCTGGCGCG
GCGAGATGCCCCGCTGGCCGGCGCCGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCGCCGCCGTTTTTGCGTTGCTATCGTGCGTTCCACCGACGACGGGCGGCTACGGGCGAAGGCCCGGGCCGCCCGTCTCC
AACCTTGCGAGGCCGATATC

Downstream 100 bases:

>100_bases
TGCGGTCGAGCCGGCGCGCCGGCGTCGCACGCTTGCCCGCCTATTTTTTCCCGCCCTTTGGGCCCATTCATCGTGATACC
GCCTCCGGTCTCGCCGGGCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSELLDLSACSLAERFKNRSVTPVDYAHALLDHIARWEPHLNALCRFDPARVRDEALASTRRWAAGAPLSEIDGVPVTIK
ELIATQGDRVAQGSAAAADAPPATVDAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGA
AAAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIEPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
PETIDWSIAPAHVKGMRIGLMLDAGCGLAPDPQIVAAVEAAAERFAAHGARIVAAPPVLSRPMLEGLDRFWRARLWADLE
RLPDAARARILPYIAEWAGQGASVSGVEAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEH
IAFTVPWNMGEQPALSINCGFARDGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAPPR

Sequences:

>Translated_464_residues
MSELLDLSACSLAERFKNRSVTPVDYAHALLDHIARWEPHLNALCRFDPARVRDEALASTRRWAAGAPLSEIDGVPVTIK
ELIATQGDRVAQGSAAAADAPPATVDAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGA
AAAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIEPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
PETIDWSIAPAHVKGMRIGLMLDAGCGLAPDPQIVAAVEAAAERFAAHGARIVAAPPVLSRPMLEGLDRFWRARLWADLE
RLPDAARARILPYIAEWAGQGASVSGVEAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEH
IAFTVPWNMGEQPALSINCGFARDGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAPPR
>Mature_463_residues
SELLDLSACSLAERFKNRSVTPVDYAHALLDHIARWEPHLNALCRFDPARVRDEALASTRRWAAGAPLSEIDGVPVTIKE
LIATQGDRVAQGSAAAADAPPATVDAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAA
AAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIEPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLPP
ETIDWSIAPAHVKGMRIGLMLDAGCGLAPDPQIVAAVEAAAERFAAHGARIVAAPPVLSRPMLEGLDRFWRARLWADLER
LPDAARARILPYIAEWAGQGASVSGVEAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHI
AFTVPWNMGEQPALSINCGFARDGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAPPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=25.7545271629779, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=25.5578093306288, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI166795287, Length=128, Percent_Identity=42.1875, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=24.7464503042596, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=241, Percent_Identity=31.5352697095436, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=256, Percent_Identity=28.90625, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=453, Percent_Identity=24.2825607064018, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=258, Percent_Identity=27.1317829457364, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=257, Percent_Identity=27.2373540856031, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=444, Percent_Identity=23.1981981981982, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=225, Percent_Identity=28.4444444444444, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=435, Percent_Identity=23.9080459770115, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=25.1243781094527, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=219, Percent_Identity=27.8538812785388, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21356731, Length=476, Percent_Identity=26.6806722689076, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=26.7465069860279, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=26.3366336633663, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648435, Length=280, Percent_Identity=28.9285714285714, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=280, Percent_Identity=28.9285714285714, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550774, Length=280, Percent_Identity=28.9285714285714, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=24.8543689320388, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=236, Percent_Identity=30.9322033898305, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=236, Percent_Identity=30.9322033898305, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652985, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922090, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24652981, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24652983, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49454; Mature: 49323

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLDLSACSLAERFKNRSVTPVDYAHALLDHIARWEPHLNALCRFDPARVRDEALAST
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
RRWAAGAPLSEIDGVPVTIKELIATQGDRVAQGSAAAADAPPATVDAPAAARLREAGAVV
HHHCCCCCHHHHCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEE
IGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAAAAAAAGYGPLHVGTDIGGS
EECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEECCCCCCE
IRLPAGWCGIVGFKPSNGRIPIEPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
EECCCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PETIDWSIAPAHVKGMRIGLMLDAGCGLAPDPQIVAAVEAAAERFAAHGARIVAAPPVLS
CCCCCCCCCCHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHH
RPMLEGLDRFWRARLWADLERLPDAARARILPYIAEWAGQGASVSGVEAVRGFGATFEMR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHH
AAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHIAFTVPWNMGEQPALSINCG
HHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCEEEEECC
FARDGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAPPR
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SELLDLSACSLAERFKNRSVTPVDYAHALLDHIARWEPHLNALCRFDPARVRDEALAST
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
RRWAAGAPLSEIDGVPVTIKELIATQGDRVAQGSAAAADAPPATVDAPAAARLREAGAVV
HHHCCCCCHHHHCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEE
IGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAAAAAAAGYGPLHVGTDIGGS
EECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEECCCCCCE
IRLPAGWCGIVGFKPSNGRIPIEPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
EECCCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PETIDWSIAPAHVKGMRIGLMLDAGCGLAPDPQIVAAVEAAAERFAAHGARIVAAPPVLS
CCCCCCCCCCHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHH
RPMLEGLDRFWRARLWADLERLPDAARARILPYIAEWAGQGASVSGVEAVRGFGATFEMR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHH
AAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHIAFTVPWNMGEQPALSINCG
HHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCEEEEECC
FARDGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAPPR
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA