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The map label for this gene is nuoB2 [H]

Identifier: 126454466

GI number: 126454466

Start: 3504167

End: 3504955

Strand: Direct

Name: nuoB2 [H]

Synonym: BURPS1106A_3598

Alternate gene names: 126454466

Gene position: 3504167-3504955 (Clockwise)

Preceding gene: 126452709

Following gene: 126453751

Centisome position: 87.86

GC content: 66.16

Gene sequence:

>789_bases
ATGCTGCTCCGGTTCGAGTCGCAAGCGGCGAGCGGCGCGCTGACTTCACTGGCCGGCAGCCCGCCGCGCACCGAAATCTC
CTCGGCCGACGTCTGCTCCGCAAAAACGCGTGCAGCCTGCCTCGATCAATCGGCGCACATACACCCGCTACCGCGGGCAT
CCGTATCGCGCTGCCGTCTTCGCTTTACGCCGCGCGCCCCTCGCTCGCGTCGCATTCGTACAAGCCATTCGGCACGACCG
CGTATAGAGTCTGCGCTCGTCACCGGCTCTTCGACTCCATTCGGCATGGCGAATCATCCCCTCACCCTCGAAAAAGACGG
CTTCATCGTCACGACGCTCGACGCCGCGATGGCCGCCGCGCAGAAAAACAGCCTCTGGTACATGACGTTCGGCCTCGCGT
GTTGCGCGGTCGAGATGATGCACGCGGCGGGCGCGCGTTACGACATGGACCGTTTCGGCATGATCCCGCGCGCGTCGCCG
CGCCAGTGCGATCTGATGATCGTCGCCGGCACGCTGACCAACAAGATGGCGCCCGCGATGCGCCGCGTCTACGACCAGAT
GGCCGAACCGCGCTACGTCGTGTCGATGGGCTCGTGCGCGAACGGCGGCGGCTATTACCACTACAGCTATTCGGTCGTGC
GCGGCTGCGATCGCATCGTGCCCGTCGACGTCTACGTGCCCGGCTGCCCGCCGACCGCCGAGGCGCTCGTCTACGGTCTG
ATGCAACTGCAGCGCAAGGTCGCCGAGCGCAGCACGCATAGTCGGCCTAAACTGTTCGCGCGCCCCTGA

Upstream 100 bases:

>100_bases
CGTCGCGGTGAAGATCGCCGGGTGCGCGCTCGCTTGCCGGTCGACGCCCCGCTGCCGCTCGCGCTCGACGACGAGCCGGT
CGCGCCAGGCGCAAAAATAC

Downstream 100 bases:

>100_bases
CCGCCGACAATCCATGCCCGCCTATCGACGACTCCACGCGCCTCACCCGCGCCTGCGCGACATCGTGAACTGCTATTTCG
TCGAGCTCGGGCGCGCGGGC

Product: putative NADH-quinone oxidoreductase, B subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2 [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLLRFESQAASGALTSLAGSPPRTEISSADVCSAKTRAACLDQSAHIHPLPRASVSRCRLRFTPRAPRSRRIRTSHSARP
RIESALVTGSSTPFGMANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASP
RQCDLMIVAGTLTNKMAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGL
MQLQRKVAERSTHSRPKLFARP

Sequences:

>Translated_262_residues
MLLRFESQAASGALTSLAGSPPRTEISSADVCSAKTRAACLDQSAHIHPLPRASVSRCRLRFTPRAPRSRRIRTSHSARP
RIESALVTGSSTPFGMANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASP
RQCDLMIVAGTLTNKMAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGL
MQLQRKVAERSTHSRPKLFARP
>Mature_262_residues
MLLRFESQAASGALTSLAGSPPRTEISSADVCSAKTRAACLDQSAHIHPLPRASVSRCRLRFTPRAPRSRRIRTSHSARP
RIESALVTGSSTPFGMANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASP
RQCDLMIVAGTLTNKMAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGL
MQLQRKVAERSTHSRPKLFARP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=160, Percent_Identity=71.25, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=49.0445859872611, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1789074, Length=159, Percent_Identity=32.0754716981132, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=38.8429752066116, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=171, Percent_Identity=60.233918128655, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI18859983, Length=161, Percent_Identity=66.4596273291926, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24642371, Length=161, Percent_Identity=66.4596273291926, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=75, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 28636; Mature: 28636

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRFESQAASGALTSLAGSPPRTEISSADVCSAKTRAACLDQSAHIHPLPRASVSRCRL
CEEEECCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEE
RFTPRAPRSRRIRTSHSARPRIESALVTGSSTPFGMANHPLTLEKDGFIVTTLDAAMAAA
EECCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCEEEECCCEEEEEHHHHHHHH
QKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASPRQCDLMIVAGTLTNKMAPAM
HCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHH
RRVYDQMAEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGL
HHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
MQLQRKVAERSTHSRPKLFARP
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLLRFESQAASGALTSLAGSPPRTEISSADVCSAKTRAACLDQSAHIHPLPRASVSRCRL
CEEEECCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEE
RFTPRAPRSRRIRTSHSARPRIESALVTGSSTPFGMANHPLTLEKDGFIVTTLDAAMAAA
EECCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCEEEECCCEEEEEHHHHHHHH
QKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASPRQCDLMIVAGTLTNKMAPAM
HCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHH
RRVYDQMAEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGL
HHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
MQLQRKVAERSTHSRPKLFARP
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA