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The map label for this gene is ubiE [H]

Identifier: 126454075

GI number: 126454075

Start: 658246

End: 658977

Strand: Direct

Name: ubiE [H]

Synonym: BURPS1106A_0683

Alternate gene names: 126454075

Gene position: 658246-658977 (Clockwise)

Preceding gene: 126451504

Following gene: 126454670

Centisome position: 16.5

GC content: 60.25

Gene sequence:

>732_bases
ATGAGCAAAACCCACTTCGGCTTCGAAACCGTCGAGGAAAACGAAAAAGCGAAGAAGGTCGCGGGGGTGTTTCACTCGGT
CGCGAGCAACTACGATCTGATGAACGACCTGATGTCGGCGGGCCTGCACCGCGCGTGGAAGGCGTTCACGATCGCGCAGG
CGAACGTGCGCCCGGGCGGCAAGGTGCTCGACATCGCAGCCGGCACGGGCGACCTGACGAAGGCGTTCGCGAAGGCGGCG
GGGCCGACGGGCGAGGTCTGGCATACCGACATCAACGAATCGATGCTGCGCGTCGGCCGCGACCGCCTGCTCGACAAGGG
CGTCGTCACGCCGTCGCTGCTCTGCGACGCGGAGAAGCTGCCGTTCCCGGACAACTATTTCGATGTGGTCACCGTCGCGT
TCGGCCTGCGCAACATGACGCACAAGGATTCGGCGCTTGCCGAGATGCGCCGCGTCGCGAAGCCGGGCGGGCGCGTGATG
GTGCTCGAGTTCTCGAAAGTCTGGGAGCCGCTGAAGAAAGCGTACGACGTCTATTCTTTCAAAGTATTACCGTGGCTTGG
CGACAAGTTCGCGAAAGATGCCGACAGTTACCGGTATCTTGCTGAATCTATCCGGATGCACCCGGATCAGGAGACGCTGA
AGACGATGATGGAACAAGCGGGCCTCGATGCCGTCAAATATTACAATTTGTCAGGTGGCGTGGTAGCTTTACACGTGGGA
ACCAAGTATTAA

Upstream 100 bases:

>100_bases
AGCGGGACGCGCCGATGCCGGCGGACTCGCTGCCGCGAGGCCACCACCACTGAACGACGGCGCCGGCGGCGCCGTTTGCC
ATTGATAGAGGATCAACGCG

Downstream 100 bases:

>100_bases
GGGGTTCCATCCATACATTTTCCTTTCAGTTGGAGGCCGGGATGTCCGTATCACGTTCGTCGTTTAACCGCAGCAGAGAG
CCGAAGTCGTTGGCGCGGCG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSKTHFGFETVEENEKAKKVAGVFHSVASNYDLMNDLMSAGLHRAWKAFTIAQANVRPGGKVLDIAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKLPFPDNYFDVVTVAFGLRNMTHKDSALAEMRRVAKPGGRVM
VLEFSKVWEPLKKAYDVYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHVG
TKY

Sequences:

>Translated_243_residues
MSKTHFGFETVEENEKAKKVAGVFHSVASNYDLMNDLMSAGLHRAWKAFTIAQANVRPGGKVLDIAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKLPFPDNYFDVVTVAFGLRNMTHKDSALAEMRRVAKPGGRVM
VLEFSKVWEPLKKAYDVYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHVG
TKY
>Mature_242_residues
SKTHFGFETVEENEKAKKVAGVFHSVASNYDLMNDLMSAGLHRAWKAFTIAQANVRPGGKVLDIAAGTGDLTKAFAKAAG
PTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKLPFPDNYFDVVTVAFGLRNMTHKDSALAEMRRVAKPGGRVMV
LEFSKVWEPLKKAYDVYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHVGT
KY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=44.043321299639, Blast_Score=223, Evalue=1e-58,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=62.5514403292181, Blast_Score=310, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=44.672131147541, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=42.0233463035019, Blast_Score=168, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=46.6666666666667, Blast_Score=223, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26955; Mature: 26824

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTHFGFETVEENEKAKKVAGVFHSVASNYDLMNDLMSAGLHRAWKAFTIAQANVRPGG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKL
CEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDVVTVAFGLRNMTHKDSALAEMRRVAKPGGRVMVLEFSKVWEPLKKAYDVYSF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHVG
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEC
TKY
CCC
>Mature Secondary Structure 
SKTHFGFETVEENEKAKKVAGVFHSVASNYDLMNDLMSAGLHRAWKAFTIAQANVRPGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKL
CEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDVVTVAFGLRNMTHKDSALAEMRRVAKPGGRVMVLEFSKVWEPLKKAYDVYSF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHVG
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEC
TKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA