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The map label for this gene is mhpA [H]

Identifier: 126453928

GI number: 126453928

Start: 3133763

End: 3135424

Strand: Reverse

Name: mhpA [H]

Synonym: BURPS1106A_3216

Alternate gene names: 126453928

Gene position: 3135424-3133763 (Counterclockwise)

Preceding gene: 126453943

Following gene: 126454817

Centisome position: 78.61

GC content: 71.12

Gene sequence:

>1662_bases
ATGACCATCGATTATCAGACGCTGAAGTTCGACTATCGCCCGCGCGCGGCGCTCGCCGATGCGGGCGAGCCGCATCCGGC
CGTCGTCGTCGGCGCGGGGCCCGTCGGGCTCGCGGCGGCGATCGACCTCGCGCAGCAGGGCGTGCCCGTCGTGCTGCTCG
ACGACGACGACACGCTGTCGAGCGGCTCGCGCGCGATCTGTTTCGCGAAGCGCACGCTCGAAATCTTCGATCGCCTCGGC
TGCGGCGAGCGGGTCGCGCAAAAGGGCGTGGGCTGGCACGTCGGCAAGGTGTTCCTGCAGGACGAGCTGATCTACGCGTT
CGACCTGCTGCCGGAAGTCGGGCATGCGCGGCCCGCGTTCGTCAATCTGCAGCAGTATTACGTCGAAGGCTATCTGGCCG
AGCGCGCGCTCGAGCTGCCGAATCTCGATCTGCGCTGGAAGAACCGCGTGACCGGAATCCGGCAGACGCCCGAGCACGCG
GAGCTCGACGTCGACACGCCGGAGGGGCCGCACACGCTGCGCGCGCGCTACGTGATCGCGGCGGACGGCTCGAAGAGCCC
GTTGCGCGCGATGATGGGGCTCGACAGCCGCGGCCGCACGTTCAAGGACCGCTTCCTGATCGCCGACGTGAAGATGAAAG
CGCCGTTCCCGGCCGAGCGCTGGTTCTGGTTCGATCCGCCGTTCCATCGCAACCAGTCGGTGCTGCTGCACCGCCAGCCG
GACGACATGTGGCGCATCGATTTCCAGCTCGGCTGGGACGCCGATCCCGTCGCCGAGAAGGCGCCGGAGCGCGTGATCCC
GCGCGTGCGCGCGCTGCTCGGGCCGGACGTCGAGTTCGAGCTCGAATGGGTGAGCGTCTATACGTTCCGCTGCCAGCGGA
TGGACAGCTTTCGCCACGGCCGCGTGCTGTTCGCGGGCGACGCCGCGCACGGCGTGTCGCCGTTCGGCGCGCGCGGCGCG
AACAGCGGCGTGCAGGACGCGGACAACCTCGCGTGGAAGCTCAAGCTCGTGCTCGACGGCCGCGCGCCCGATCGTCTGCT
CGACACGTATGCGCGCGAGCGCGAATTCGCGGCCGACGAGAACATCCGCAATTCGACGCGCTCGACCGATTTCATCACGC
CGAAGAGCCCGATCTCGCGGGTGTTTCGCGATGCGACGCTCAAGCTCGCGCGCGATTGCGAGTTCGCGCGCCGGCTCGTC
AACAGCGGCCGGCTGTCGGTGCCGGCCGTGCTCGCCGATTCGCCGCTGAACACGCCCGATCGCGCGGGCGAGGCGTTCGA
GCGCGCGCCGGTGCCGGGCGCGGTCGCCCCCGATGCGCCCGTGCGCGCGAACGGCGAGCCCGGCTGGCTGCTGCAGCATC
TGCAGGGCGGCTTCGTCGGCGTCCTGTTCGGCTCGATCGGCGATGCGGGCGCGCTCGTCGACGCGACGCGCGGCCACGCG
CTGCCGGTGGCGCCCGTGCTCATCGTGCCGAAGGGCGAGGCGCGCGCGGTCGCGGGCGTCGACGTGATCGAGGACAGCGA
GGGGCTCGTCGCGCGGCGCTACGACGCGCGCCCCGGCACGTTCTATCTGCTGCGCCCGGACCAGCACGTGTGCGCGCGGT
TTCGCACGGCGCAAGCGAGCGCGGTGCGCGCGGCGCTCGCCCGCGCGATCTGCGATGCGTGA

Upstream 100 bases:

>100_bases
GACGAGGCCACGCAATACCCGGATCCGCGGATCTGGACGGCCGAGCGCGACAAGGACATGTGGCACACCCTCGAAGGCTG
AGGCACGGCGGGCGGCGATC

Downstream 100 bases:

>100_bases
CGTGAGCGCAAAGACTCGGTTAAAGACATCAGGAGACCCACGATGCACCTCGACACCCGCTTGCGCCTGCCCGATCCGGA
TGCGTTCTACGAGGCGCTGA

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLG
CGERVAQKGVGWHVGKVFLQDELIYAFDLLPEVGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKNRVTGIRQTPEHA
ELDVDTPEGPHTLRARYVIAADGSKSPLRAMMGLDSRGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDAAHGVSPFGARGA
NSGVQDADNLAWKLKLVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLV
NSGRLSVPAVLADSPLNTPDRAGEAFERAPVPGAVAPDAPVRANGEPGWLLQHLQGGFVGVLFGSIGDAGALVDATRGHA
LPVAPVLIVPKGEARAVAGVDVIEDSEGLVARRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICDA

Sequences:

>Translated_553_residues
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLG
CGERVAQKGVGWHVGKVFLQDELIYAFDLLPEVGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKNRVTGIRQTPEHA
ELDVDTPEGPHTLRARYVIAADGSKSPLRAMMGLDSRGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDAAHGVSPFGARGA
NSGVQDADNLAWKLKLVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLV
NSGRLSVPAVLADSPLNTPDRAGEAFERAPVPGAVAPDAPVRANGEPGWLLQHLQGGFVGVLFGSIGDAGALVDATRGHA
LPVAPVLIVPKGEARAVAGVDVIEDSEGLVARRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICDA
>Mature_552_residues
TIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLGC
GERVAQKGVGWHVGKVFLQDELIYAFDLLPEVGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKNRVTGIRQTPEHAE
LDVDTPEGPHTLRARYVIAADGSKSPLRAMMGLDSRGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQPD
DMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDAAHGVSPFGARGAN
SGVQDADNLAWKLKLVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVN
SGRLSVPAVLADSPLNTPDRAGEAFERAPVPGAVAPDAPVRANGEPGWLLQHLQGGFVGVLFGSIGDAGALVDATRGHAL
PVAPVLIVPKGEARAVAGVDVIEDSEGLVARRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICDA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=515, Percent_Identity=25.8252427184466, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60889; Mature: 60758

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLS
CCCCCEEEEECCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCC
SGSRAICFAKRTLEIFDRLGCGERVAQKGVGWHVGKVFLQDELIYAFDLLPEVGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEHHHEEHHHHHHHHHHHHHHHCCCCCCE
VNLQQYYVEGYLAERALELPNLDLRWKNRVTGIRQTPEHAELDVDTPEGPHTLRARYVIA
EHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEE
ADGSKSPLRAMMGLDSRGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
ECCCCCHHHHHHCCCCCCCEEECEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEECC
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMDSFRHG
CCEEEEEEEECCCCCHHHHHCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHCCCCCC
RVLFAGDAAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAPDRLLDTYAREREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
NIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVNSGRLSVPAVLADSPLNTPD
CHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCH
RAGEAFERAPVPGAVAPDAPVRANGEPGWLLQHLQGGFVGVLFGSIGDAGALVDATRGHA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCC
LPVAPVLIVPKGEARAVAGVDVIEDSEGLVARRYDARPGTFYLLRPDQHVCARFRTAQAS
CCCCCEEEEECCCCCEEECCEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
AVRAALARAICDA
HHHHHHHHHHHCC
>Mature Secondary Structure 
TIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLS
CCCCEEEEECCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCC
SGSRAICFAKRTLEIFDRLGCGERVAQKGVGWHVGKVFLQDELIYAFDLLPEVGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEHHHEEHHHHHHHHHHHHHHHCCCCCCE
VNLQQYYVEGYLAERALELPNLDLRWKNRVTGIRQTPEHAELDVDTPEGPHTLRARYVIA
EHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEE
ADGSKSPLRAMMGLDSRGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
ECCCCCHHHHHHCCCCCCCEEECEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEECC
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMDSFRHG
CCEEEEEEEECCCCCHHHHHCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHCCCCCC
RVLFAGDAAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAPDRLLDTYAREREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
NIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVNSGRLSVPAVLADSPLNTPD
CHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCH
RAGEAFERAPVPGAVAPDAPVRANGEPGWLLQHLQGGFVGVLFGSIGDAGALVDATRGHA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCC
LPVAPVLIVPKGEARAVAGVDVIEDSEGLVARRYDARPGTFYLLRPDQHVCARFRTAQAS
CCCCCEEEEECCCCCEEECCEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
AVRAALARAICDA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA