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The map label for this gene is gshA [H]

Identifier: 126453055

GI number: 126453055

Start: 128599

End: 130212

Strand: Direct

Name: gshA [H]

Synonym: BURPS1106A_0134

Alternate gene names: 126453055

Gene position: 128599-130212 (Clockwise)

Preceding gene: 126452165

Following gene: 126454673

Centisome position: 3.22

GC content: 67.72

Gene sequence:

>1614_bases
ATGCCGAACAACATGACTTCCCGCCAGACAGACTTGTTGCAGCAACGCCTTGCCGTGTTGAGCACGAGCCCGACGCGCGA
GCAGCTGCCTGACGGCCTGCGCGGCATCGAGAAGGAAAGCCTGCGCGTGACGCGCGACGGGATGATCGCGTTTACGCCGC
ATCCGCGCGCGCTCGGCTCGGCGCTCACGCATCCGTCGCTGACGACCGATTATTCGGAGGCGCTCGTCGAGCTGATCACG
CCTGCGGAGCGCGACGCGGCGACGACGCTCGAGCGGCTCGACGAGCTGCATCGCTACGTCTACGCGTCGCTCGGCGACGA
GATGCTGTGGACCGATTCGATGCCGGGCCTGCTGCCGGCCGACGACGAAATCCCGATCGCGGACTACGGCACGTCGAACA
TCGGGCGGCTGAAGACCGTCTACCGGCGCGGCCTCGCGTATCGCTACGGCCGCACGATGCAGTGCATCGCGGGGATTCAC
TACAACTATTCGCTGAGCGAGGAAGTGTGGCGGCGGCTGCACGCGGAGGAAGGCTCGACGGCGGGCATCGTCGATTACCA
GTCCGAGCGCTACCTCGCGCTGATCCGCAATTTCCGCCGCACGAGCTGGCTGCTGATGTACCTGTTCGGCGCGTCGCCCG
CGCTCGATGCCGGCTTCCTGCGCGGCCGAGCGCACAAGCTCGACACGTTCGACGCCGCGACGCTGTATCGCCCGCACGCG
ACGAGCCTGCGGATGAGCGACCTCGGCTACTCGAACACGACCGCGCAAGCCGCGCTGCAGGCGGACTACAACACGCTGTC
CGGCTATCTCGACGCGCTGTCGAAGGCGGTCAGCGAGCCGTATCCGCCGTACGAGGCGATCGGCACGCACCGCGACGGCG
AGTGGATTCAGATCAACACGAACGTGCTGCAGATCGAGAACGAGTTCTACTCGACGATCCGGCCGAAGCGCGTCACGTAT
TCGGGCGAGCGGCCGCTGCACGCGCTTGCGTCGCGCGGCGTTCAGTACATCGAGGTGCGCTGTCTCGACATCGATCCGTT
CGAGCCGACGGGCATCGCGCTCGACACCGCGCGCTTTCTCGACGCGTACCTGCTCGTCTGCGCGCTCGACGAGAGCGCGC
CGCTCACGTGCGATGCGTATCGCGAGTCGAATGCGAACTTCGGCCGCGTGACGATGGAAGGGCGCAAGCCGGGGCTCGAG
CTGACGCGCGACGGCGCGGCCGTGCCGATGCGTGCATGGGCCGACGAGATCTTCGCGCAGATCGAAGCCGCGGGCCGCGT
GCTCGACGACATCCGCGGCGACGACACGCATGCGCGCGCGATCGCCGCGCAGCGCGCGAAGCTGGACGATCCTGAGCTCA
CGTCTTCCGCGCGCGTGCTGCGCGCGATGCGCGAGAACGGACAGTCGTTCCTCGAGTTCGCACGTGCGCAAAGCGCCGCG
CATGCGGCGTATTTCCATGGCCGCCCGCTCGACGAAGCCGCGACGCGCGACGCGCGGGCGCTCGCCGAGCGCTCGCTTGC
CGAGCAGGCCGAACTCGAAGGCAAGAACGCCGGGTCGTTCGACGCGTTCGTCGCTGCTTACCGCGCCTATACGCTGAACC
GCTTCAGCGTGTGA

Upstream 100 bases:

>100_bases
TCAAGCGAGCCTTTCGGCGAGATGTTAAAGTGCCCGCTACTGTCAAATCCCGCCAGGTGATTCGCGCGTGCCGCGGATCG
CCGAGCCCGCACTTGCATCA

Downstream 100 bases:

>100_bases
CGCGCGGCGGGCGTTACGGCTGCACGTCGAGGAAGTCGTGCGCGTTGCACGCTTCGGCCGTCACGCGCAGCGTCTGCAGC
GCGCGCTTCTCGATGCGGCG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALVELIT
PAERDAATTLERLDELHRYVYASLGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLSEEVWRRLHAEEGSTAGIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDAGFLRGRAHKLDTFDAATLYRPHA
TSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLE
LTRDGAAVPMRAWADEIFAQIEAAGRVLDDIRGDDTHARAIAAQRAKLDDPELTSSARVLRAMRENGQSFLEFARAQSAA
HAAYFHGRPLDEAATRDARALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_537_residues
MPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALVELIT
PAERDAATTLERLDELHRYVYASLGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLSEEVWRRLHAEEGSTAGIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDAGFLRGRAHKLDTFDAATLYRPHA
TSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLE
LTRDGAAVPMRAWADEIFAQIEAAGRVLDDIRGDDTHARAIAAQRAKLDDPELTSSARVLRAMRENGQSFLEFARAQSAA
HAAYFHGRPLDEAATRDARALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
>Mature_536_residues
PNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALVELITP
AERDAATTLERLDELHRYVYASLGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHY
NYSLSEEVWRRLHAEEGSTAGIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDAGFLRGRAHKLDTFDAATLYRPHAT
SLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYS
GERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLEL
TRDGAAVPMRAWADEIFAQIEAAGRVLDDIRGDDTHARAIAAQRAKLDDPELTSSARVLRAMRENGQSFLEFARAQSAAH
AAYFHGRPLDEAATRDARALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=521, Percent_Identity=40.8829174664108, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59846; Mature: 59715

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRYVYASLGDEMLWTDSMPGLLPA
HHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
DDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEVWRRLHAEEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
AGIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDAGFLRGRAHKLDTFDAATLYRPHA
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHEECCCC
TSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINT
CCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFL
CEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEHHHHHHH
DAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELTRDGAAVPMRAWADEIFAQ
HHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHH
IEAAGRVLDDIRGDDTHARAIAAQRAKLDDPELTSSARVLRAMRENGQSFLEFARAQSAA
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
HAAYFHGRPLDEAATRDARALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRYVYASLGDEMLWTDSMPGLLPA
HHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
DDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEVWRRLHAEEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
AGIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDAGFLRGRAHKLDTFDAATLYRPHA
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHEECCCC
TSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINT
CCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFL
CEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEHHHHHHH
DAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELTRDGAAVPMRAWADEIFAQ
HHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHH
IEAAGRVLDDIRGDDTHARAIAAQRAKLDDPELTSSARVLRAMRENGQSFLEFARAQSAA
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
HAAYFHGRPLDEAATRDARALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA