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The map label for this gene is alkA [H]

Identifier: 126452165

GI number: 126452165

Start: 127526

End: 128440

Strand: Direct

Name: alkA [H]

Synonym: BURPS1106A_0133

Alternate gene names: 126452165

Gene position: 127526-128440 (Clockwise)

Preceding gene: 126454639

Following gene: 126453055

Centisome position: 3.2

GC content: 74.64

Gene sequence:

>915_bases
GTGCGGCCCGACAGCGTCGTGCTCGAGCTGCCGTTCAAATCGCCGTACGATTGGCCGCGCGTGCTGCGCTTCTTCGCGGG
GCGCGCGATTCCGGGCGTCGAGGCGGTCGGGGACGGCGCGTATCGGCGCACGGTCGACCATCACGGCGCGATCGGCACGT
TGACGGTGCGCAAGCATCCGCGCAAGCGCTGTCTTGTCGCGCTCGTCGAGGGCGATGCGGCGCGGCATGCGGACGCTGCA
TTCGCCGCGCGGCTTTCGACGATGTTCGATTTGCAGGCCGATCCGGCCGCGATCGGCGCGCATCTCGCGCGCGACGCGTG
GTTCGCGCCGCTCGTCGGCGCCGCGCCCGGCCTGCGCGTGCCGGGCGCGTGGTCGGGCTTCGAGCTGATCGTGCGCGCGA
TCGTCGGCCAGCAGGTGAGCGTGAAGGCCGCGACGACGATCGTCGGGCGGCTCGTCGAGCGCGCGGGCGAGCGGCTTGCC
CCGCACGCGCCGGGCGCGATTGGCTGGCGGTTTCCGGAGCCCGCCGCGCTCGCCGCGTGCGACGTGTCGCGCATCGGGAT
GCCGGGCAAGCGTGCCGCGGCGCTGCAGGGCGTCGCGCGCGCGGTCGCCTCGGGCGACGTGCCGCTCGATGCGTACGCGA
GCGATCCGGCCGGCGTGCGCGCCGCGCTGCTCGCGCTGCCGGGGATCGGCCCGTGGACCGTCGAATACGTCGCGATGCGC
GCATGGCGCGACGCCGACGCATGGCCCGCGACCGACCTCGTGCTGATGCAGGCGATCGTCGCGCGCGACCCGGCGCTAGA
CCGGCCGGCAAGCCAGCGCCTGCGCGCCGATGCGTGGCGGCCGTGGCGCGCGTATGCGGCGATGCATCTATGGAACGAGA
TCGCCGATCGCGCCGGTTCGGCGCGCGGCGGATAG

Upstream 100 bases:

>100_bases
AGGCGGCGCTCCTCGATGCCGAGGCGCAGCGCGCGGCGGGCGAATCCGTCGAATCCGCCGTGGACCACGCAGCTTGAGCG
CCGCCGTCGAACCGGCCGCC

Downstream 100 bases:

>100_bases
GCGCGCGCGCCCGCCCCGCGCGGCGCGCGCGAATCGACAGGCAATGCCGAAGGGCAGGTCAAGCGAGCCTTTCGGCGAGA
TGTTAAAGTGCCCGCTACTG

Product: DNA-3-methyladenine glycosylase 2

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRPDSVVLELPFKSPYDWPRVLRFFAGRAIPGVEAVGDGAYRRTVDHHGAIGTLTVRKHPRKRCLVALVEGDAARHADAA
FAARLSTMFDLQADPAAIGAHLARDAWFAPLVGAAPGLRVPGAWSGFELIVRAIVGQQVSVKAATTIVGRLVERAGERLA
PHAPGAIGWRFPEPAALAACDVSRIGMPGKRAAALQGVARAVASGDVPLDAYASDPAGVRAALLALPGIGPWTVEYVAMR
AWRDADAWPATDLVLMQAIVARDPALDRPASQRLRADAWRPWRAYAAMHLWNEIADRAGSARGG

Sequences:

>Translated_304_residues
MRPDSVVLELPFKSPYDWPRVLRFFAGRAIPGVEAVGDGAYRRTVDHHGAIGTLTVRKHPRKRCLVALVEGDAARHADAA
FAARLSTMFDLQADPAAIGAHLARDAWFAPLVGAAPGLRVPGAWSGFELIVRAIVGQQVSVKAATTIVGRLVERAGERLA
PHAPGAIGWRFPEPAALAACDVSRIGMPGKRAAALQGVARAVASGDVPLDAYASDPAGVRAALLALPGIGPWTVEYVAMR
AWRDADAWPATDLVLMQAIVARDPALDRPASQRLRADAWRPWRAYAAMHLWNEIADRAGSARGG
>Mature_304_residues
MRPDSVVLELPFKSPYDWPRVLRFFAGRAIPGVEAVGDGAYRRTVDHHGAIGTLTVRKHPRKRCLVALVEGDAARHADAA
FAARLSTMFDLQADPAAIGAHLARDAWFAPLVGAAPGLRVPGAWSGFELIVRAIVGQQVSVKAATTIVGRLVERAGERLA
PHAPGAIGWRFPEPAALAACDVSRIGMPGKRAAALQGVARAVASGDVPLDAYASDPAGVRAALLALPGIGPWTVEYVAMR
AWRDADAWPATDLVLMQAIVARDPALDRPASQRLRADAWRPWRAYAAMHLWNEIADRAGSARGG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=292, Percent_Identity=36.6438356164384, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32396; Mature: 32396

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPDSVVLELPFKSPYDWPRVLRFFAGRAIPGVEAVGDGAYRRTVDHHGAIGTLTVRKHP
CCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHCCCEEEEEECCCC
RKRCLVALVEGDAARHADAAFAARLSTMFDLQADPAAIGAHLARDAWFAPLVGAAPGLRV
HHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PGAWSGFELIVRAIVGQQVSVKAATTIVGRLVERAGERLAPHAPGAIGWRFPEPAALAAC
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DVSRIGMPGKRAAALQGVARAVASGDVPLDAYASDPAGVRAALLALPGIGPWTVEYVAMR
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
AWRDADAWPATDLVLMQAIVARDPALDRPASQRLRADAWRPWRAYAAMHLWNEIADRAGS
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
ARGG
CCCC
>Mature Secondary Structure
MRPDSVVLELPFKSPYDWPRVLRFFAGRAIPGVEAVGDGAYRRTVDHHGAIGTLTVRKHP
CCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHCCCEEEEEECCCC
RKRCLVALVEGDAARHADAAFAARLSTMFDLQADPAAIGAHLARDAWFAPLVGAAPGLRV
HHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PGAWSGFELIVRAIVGQQVSVKAATTIVGRLVERAGERLAPHAPGAIGWRFPEPAALAAC
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DVSRIGMPGKRAAALQGVARAVASGDVPLDAYASDPAGVRAALLALPGIGPWTVEYVAMR
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
AWRDADAWPATDLVLMQAIVARDPALDRPASQRLRADAWRPWRAYAAMHLWNEIADRAGS
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
ARGG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]