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The map label for this gene is shiA [H]

Identifier: 126453000

GI number: 126453000

Start: 1133263

End: 1134576

Strand: Direct

Name: shiA [H]

Synonym: BURPS1106A_1154

Alternate gene names: 126453000

Gene position: 1133263-1134576 (Clockwise)

Preceding gene: 126452360

Following gene: 126452829

Centisome position: 28.41

GC content: 70.47

Gene sequence:

>1314_bases
ATGACTGCCCCCCAACCGTGCGCGCGCCAGCCGGCGCGCGCCGCTGCCGCCGCGTTCGTCGGCACGATGATCGAGTGGTA
CGACTTCTACATTTACGCGACCGCCGCCGCGCTCGTGTTCGGCGAGTTGTACTTTCCGTCGGGCGACCCGTTCGTCAGCA
CGATGGCTTCGTTCGGCACGTTCGCGGTCGGCTTTTTCGCGCGGCCGCTCGGCGGCGTCGTGTTCGGCCATCTCGGCGAC
CGGATCGGCCGCAAGAAGGCGCTGATGACGACGCTCGCGATGATGGGCGCGGCGACCGTGTGCATCGGCCTGTTGCCGAG
CCATGCGTCGATCGGTGCGCTCGCGCCGGTGCTGCTCGTGCTGCTGCGGATCGTGCAGGGCGTCGCGGTGGGCGGCGAGT
GGGGCGGCGCGGTGCTGATGGCGGGCGAGCACGCGCCCGACGGGCGGCGCACGTTCTTCGCTTCGTTCGCGCAGTTGGGC
AGCCCGGCCGGGCTGATCCTGTCGCTCGTCGCGTTTCGCGCGGCCACGTCGCTCGAGAAGGCGGACTTTCTCGCATGGGG
CTGGCGCCTGCCGTTTCTCGCGAGCGCGGTGCTGCTGATCGTCGGTCTCGCGATCCGGCTGGGCGTGAACGAATCGCCGG
AATTCGAGGCGGTGAAGCGCACGCGCGCCACCGCGAAGCTGCCGATCGCCGACGTGTTGCGCTCCGCGTCGGGGCTCGTG
CTGCTGTGCATCGGCGCGAACACGATCGGCATCGCCGGCGTGTATTTCACGAACACGTTCATGATCGCGTACACGACGCA
GCAGCTCGGCATCCCGCGCGCGACGATCCTCGATTGCCTGTTCGCGGTCGCGATCATCCAGTTCGTCGCGCAGCCCGCCG
CCGCGTGGCTGGCCGAAAAGCTCGGCGGCGCGCGTTTCCTGAAGCTCGCGGCCGTCGCCGCGATGGCTTCGCCGTATCCG
ATGTTCATGCTCGTGACGAGCGGGCGGCCCGGCGCGATCGTCGCCGGCATCGCGCTCGCGGTGGTCTGCATGGCGGGCTT
CTATTCGGTGATCGCGGGTTTCGTCTCCGGCGTGTTCGCGGCGCGCATCCGCTATTCGGCGATCTCGCTGTCCTACCAGG
TGTGCGGCGCGCTCGCGGGCGGCCTCACGCCGCTCGTCGGCACGTGGCTCGCGCATCGCTACGCGGGCGCATGGCTGCCG
CTCGCGCTGTTCTATACGTGTCTCGCGGGGCTCTCGCTCGTGAGCGTCGTCGCGCTCGACGCGCGCCGCGCCGCGCGAGG
CGCTTCGGGCCGCGAAGCGCTGGAGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGCGTGCGGCGCGGGCCGGGCGACGGGCCCCGCGACATCGGGCGCGCGCCCGCCGAATCGGCGGGCCACGCGCATGAG
AGCAGAGAGGAGATCCCGCG

Downstream 100 bases:

>100_bases
CATGATGGCTTCGGCACTGGGCGGGCCGACGCTCGCGATCGACGGCGAGCGGCTGTGGCGCTCGCTCATGGAGATGGCGC
GCATCGGCGGCACCGCGCAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTAPQPCARQPARAAAAAFVGTMIEWYDFYIYATAAALVFGELYFPSGDPFVSTMASFGTFAVGFFARPLGGVVFGHLGD
RIGRKKALMTTLAMMGAATVCIGLLPSHASIGALAPVLLVLLRIVQGVAVGGEWGGAVLMAGEHAPDGRRTFFASFAQLG
SPAGLILSLVAFRAATSLEKADFLAWGWRLPFLASAVLLIVGLAIRLGVNESPEFEAVKRTRATAKLPIADVLRSASGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQQLGIPRATILDCLFAVAIIQFVAQPAAAWLAEKLGGARFLKLAAVAAMASPYP
MFMLVTSGRPGAIVAGIALAVVCMAGFYSVIAGFVSGVFAARIRYSAISLSYQVCGALAGGLTPLVGTWLAHRYAGAWLP
LALFYTCLAGLSLVSVVALDARRAARGASGREALEAR

Sequences:

>Translated_437_residues
MTAPQPCARQPARAAAAAFVGTMIEWYDFYIYATAAALVFGELYFPSGDPFVSTMASFGTFAVGFFARPLGGVVFGHLGD
RIGRKKALMTTLAMMGAATVCIGLLPSHASIGALAPVLLVLLRIVQGVAVGGEWGGAVLMAGEHAPDGRRTFFASFAQLG
SPAGLILSLVAFRAATSLEKADFLAWGWRLPFLASAVLLIVGLAIRLGVNESPEFEAVKRTRATAKLPIADVLRSASGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQQLGIPRATILDCLFAVAIIQFVAQPAAAWLAEKLGGARFLKLAAVAAMASPYP
MFMLVTSGRPGAIVAGIALAVVCMAGFYSVIAGFVSGVFAARIRYSAISLSYQVCGALAGGLTPLVGTWLAHRYAGAWLP
LALFYTCLAGLSLVSVVALDARRAARGASGREALEAR
>Mature_436_residues
TAPQPCARQPARAAAAAFVGTMIEWYDFYIYATAAALVFGELYFPSGDPFVSTMASFGTFAVGFFARPLGGVVFGHLGDR
IGRKKALMTTLAMMGAATVCIGLLPSHASIGALAPVLLVLLRIVQGVAVGGEWGGAVLMAGEHAPDGRRTFFASFAQLGS
PAGLILSLVAFRAATSLEKADFLAWGWRLPFLASAVLLIVGLAIRLGVNESPEFEAVKRTRATAKLPIADVLRSASGLVL
LCIGANTIGIAGVYFTNTFMIAYTTQQLGIPRATILDCLFAVAIIQFVAQPAAAWLAEKLGGARFLKLAAVAAMASPYPM
FMLVTSGRPGAIVAGIALAVVCMAGFYSVIAGFVSGVFAARIRYSAISLSYQVCGALAGGLTPLVGTWLAHRYAGAWLPL
ALFYTCLAGLSLVSVVALDARRAARGASGREALEAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=40, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=33.3333333333333, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=37.5296912114014, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788942, Length=388, Percent_Identity=30.1546391752577, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082231, Length=225, Percent_Identity=24, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45528; Mature: 45397

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPQPCARQPARAAAAAFVGTMIEWYDFYIYATAAALVFGELYFPSGDPFVSTMASFGT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGVVFGHLGDRIGRKKALMTTLAMMGAATVCIGLLPSHASIGALAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLRIVQGVAVGGEWGGAVLMAGEHAPDGRRTFFASFAQLGSPAGLILSLVAFRAATSLEK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ADFLAWGWRLPFLASAVLLIVGLAIRLGVNESPEFEAVKRTRATAKLPIADVLRSASGLV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEE
LLCIGANTIGIAGVYFTNTFMIAYTTQQLGIPRATILDCLFAVAIIQFVAQPAAAWLAEK
EEEECCCCHHHEEEHHHHEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGARFLKLAAVAAMASPYPMFMLVTSGRPGAIVAGIALAVVCMAGFYSVIAGFVSGVFA
HCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIRYSAISLSYQVCGALAGGLTPLVGTWLAHRYAGAWLPLALFYTCLAGLSLVSVVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ARRAARGASGREALEAR
HHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
TAPQPCARQPARAAAAAFVGTMIEWYDFYIYATAAALVFGELYFPSGDPFVSTMASFGT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGVVFGHLGDRIGRKKALMTTLAMMGAATVCIGLLPSHASIGALAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLRIVQGVAVGGEWGGAVLMAGEHAPDGRRTFFASFAQLGSPAGLILSLVAFRAATSLEK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ADFLAWGWRLPFLASAVLLIVGLAIRLGVNESPEFEAVKRTRATAKLPIADVLRSASGLV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEE
LLCIGANTIGIAGVYFTNTFMIAYTTQQLGIPRATILDCLFAVAIIQFVAQPAAAWLAEK
EEEECCCCHHHEEEHHHHEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGARFLKLAAVAAMASPYPMFMLVTSGRPGAIVAGIALAVVCMAGFYSVIAGFVSGVFA
HCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIRYSAISLSYQVCGALAGGLTPLVGTWLAHRYAGAWLPLALFYTCLAGLSLVSVVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ARRAARGASGREALEAR
HHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]