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The map label for this gene is kefA [H]

Identifier: 126452978

GI number: 126452978

Start: 958255

End: 959607

Strand: Direct

Name: kefA [H]

Synonym: BURPS1106A_0975

Alternate gene names: 126452978

Gene position: 958255-959607 (Clockwise)

Preceding gene: 126454034

Following gene: 126452903

Centisome position: 24.03

GC content: 70.66

Gene sequence:

>1353_bases
ATGATGACGAACCACCTCACACGGCGGCTCGCGGCCGTCGCGCACGACTTCGGCCAGCCGGCGATGATCTGGCAGGCGGT
CGCGTTCTTCGGCTCGCTCGCGCTCGCGTGGCTGCTCGCGCGCCATCTGCGCGGGCGCATCGACGCGCGCCGTCGCGCGG
CCGGGCGCACGCCCGGCCCGGGCGCGCTGAGCCTGAACCGCGCGCTGTTCCCGCTCATCGGCGGCGTGTTCATGTGGCTC
GCGGAGACGACGTTCGACGAGCTGATGCCGACGTCGCTCCTGCAGCTCGCGCTCGTGCCGCTCTTCGGCATCGGGCTCAT
CTACATCCTGTTCTTCGTCGCGCGCCGCGTGTTCGCGCGCGACGGCCACACGCACGCGTGGCTGTCGATCGTCGAGAAGC
TCGTGTCGATCGTCGTGTGGATCGCGATGGCGCTCACGGTACTCGGCATCCAGCGCGACGTGCTCGGCTGGCTCGACAGC
GTCCAGTTCCGCGTCGCGAACGCGCGCCTCACGCTGCTGTCGATCGTGTCGGGCGCGCTGTGGGTCTGCGTGACGCTCGT
CGTCGCGATGTGGCTCGGCTCGGTGCTCGAGGAGCGGCTGATGCGCTCGCGCTCGCTCGACGCGAACCTGCGGGTCGTGC
TCGCGCGCGTCGGCCGTGCGCTCCTCATCTTCGCGGCGGTGCTGATCGGGCTGTCGCTCGTCGGCATCGACGTGACCGTG
CTCGGCGTGTTCGGCGGCGCGCTCGGCGTCGGGCTCGGCTTCGGCCTGCAGAAAATTGCGAGCAACTATGTGTCGGGCTT
CATCATCCTGCTCGACCGCTCGCTGCGGCTCGGCGACGCGATCAACGTGGGCGGCCTGCAGGGCGTCGTCACGCAGATCC
GCACGCGCTACACGGTCGTGCGCGGCCTCGACGGCAACGAGACGCTGATTCCGAACGAGAAGCTCATCACCGACGTCGTG
CAGAACCAGTCGTCGTACCTGACGCGCGGCTACGCGAAGGTCGCGGTTCAGGTCGCGTACACGTCGGACGTCGAGCGCGC
GATGGCGCTGCTCGCCGACGCGGCGAAGGGCGTCGAGCGCGTGCTCGAGGATCCGGCGCCCACGCCTTATCTCGTCGGCT
TCGGCGCGGACGGCATCGATCTCGAGCTCGGCTTCTGGATCGCCGACGCGGCGAAGGGGACGGCCGGCGTGCGCTCGACG
GTCAACCGCAACATCTGGCGGCTTTTTGTCGCACACGGGATCTCGATTCCGTTCCCGCAGCGGGAGGTGCGCGTGATCGG
GCTGCCCGACGGCATCGCGCCCGCGGCCGGCGCAGCCGGCCCGGCGGCGGGGCGGCAGCCGCAGGACGCCTAG

Upstream 100 bases:

>100_bases
TCGACACGGGCGACTACCTGTCGCTCTGGCCGCATCGCCACGCGACCGACGGCTTTTTCGCCGCCGTGCTCGAGCGGCGC
GCGCAGTAACGGGCGGCGGC

Downstream 100 bases:

>100_bases
CGCGGCGGGCGGGCCGCCCGCCGCGCGCCAGCCCGAAAATCTCCGCGCGCGTATTGCGTCGCCGCAAAAAATATTCATTT
TTTACAAAGACTTGGAACGG

Product: small conductance mechanosensitive ion channel (MscS) family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MMTNHLTRRLAAVAHDFGQPAMIWQAVAFFGSLALAWLLARHLRGRIDARRRAAGRTPGPGALSLNRALFPLIGGVFMWL
AETTFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKLVSIVVWIAMALTVLGIQRDVLGWLDS
VQFRVANARLTLLSIVSGALWVCVTLVVAMWLGSVLEERLMRSRSLDANLRVVLARVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVERAMALLADAAKGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTAGVRST
VNRNIWRLFVAHGISIPFPQREVRVIGLPDGIAPAAGAAGPAAGRQPQDA

Sequences:

>Translated_450_residues
MMTNHLTRRLAAVAHDFGQPAMIWQAVAFFGSLALAWLLARHLRGRIDARRRAAGRTPGPGALSLNRALFPLIGGVFMWL
AETTFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKLVSIVVWIAMALTVLGIQRDVLGWLDS
VQFRVANARLTLLSIVSGALWVCVTLVVAMWLGSVLEERLMRSRSLDANLRVVLARVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVERAMALLADAAKGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTAGVRST
VNRNIWRLFVAHGISIPFPQREVRVIGLPDGIAPAAGAAGPAAGRQPQDA
>Mature_450_residues
MMTNHLTRRLAAVAHDFGQPAMIWQAVAFFGSLALAWLLARHLRGRIDARRRAAGRTPGPGALSLNRALFPLIGGVFMWL
AETTFDELMPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVEKLVSIVVWIAMALTVLGIQRDVLGWLDS
VQFRVANARLTLLSIVSGALWVCVTLVVAMWLGSVLEERLMRSRSLDANLRVVLARVGRALLIFAAVLIGLSLVGIDVTV
LGVFGGALGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKVAVQVAYTSDVERAMALLADAAKGVERVLEDPAPTPYLVGFGADGIDLELGFWIADAAKGTAGVRST
VNRNIWRLFVAHGISIPFPQREVRVIGLPDGIAPAAGAAGPAAGRQPQDA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=192, Percent_Identity=36.4583333333333, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI2367355, Length=318, Percent_Identity=29.559748427673, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=30.6818181818182, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48397; Mature: 48397

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]