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The map label for this gene is pucI [H]

Identifier: 126452220

GI number: 126452220

Start: 2392493

End: 2394025

Strand: Reverse

Name: pucI [H]

Synonym: BURPS1106A_2434

Alternate gene names: 126452220

Gene position: 2394025-2392493 (Counterclockwise)

Preceding gene: 126452872

Following gene: 126453356

Centisome position: 60.02

GC content: 65.1

Gene sequence:

>1533_bases
ATGACGATCGCGCGGCTCGTCCGCGGCAGGCAAGGAGATGTCATGGCTCAGTTCAGTGTGGCGCGGCAAAGCGCCTCGTA
TCGGCCGAACGAGGATCGCGCCGGCGGCCCGGATGGCGGCGCTCAGATGCCCGCCGGCTACAGCAGTCGTCTGTACAACG
AAGATCTCGCGCCGCTCGCGAGCCAGCGCTGGGGCGCATACAACATCTTCGCGTTCTGGATGTCGGACGTGCACAGCGTC
GGCGGCTACGTGTTTGCGGGCAGCCTGTTCGCGCTCGGTCTGACGAGCTGGCAGGTGCTCGTCGCGCTGATCGTCGGCAT
TTCGATCGTCAACGTGCTGTGCAACCTGATCGCGAGGCCGAGCCAGCAGCTAGGCGTGCCGTATCCGGTGGCATGCCGCG
CGACGTTCGGCGTGCTCGGCGCGAACGTGCCCGCGGTGATCCGCGGCCTCATCGCGATCGCATGGTACGGCATCCAAACT
TATCTGGCGTCGAGCGCGCTCGTGATCGTCGTGCTCAAGTTCTTTCCGCACTGGATGCCGTACGCGGACGTGCATCGCTA
CGGCTTTCTCGGGCTGTCGGCGCTCGGCTGGGCGGGCTTCATGCTGCTGTGGGTGCTGCAGGCGTTCGTGTTCTGGAACG
GCATGGAGACGATCAAGAAGTTCATCGATTTCGCCGGCCCCGCCGTCTACGTGGTGATGTTCGCCCTCGCGGGCTACATG
GTATGGCGCGCGGGCTGGCGCAATATCGGCCTGAATCTCGGCGGCGTCCGGTATCACGGCGCCGAAGTGATTCCGGTGAT
GGTGACGGCGATCTCGCTCGTCGTGTCGTATTTCTCGGGGCCGATGCTCAACTTCGGCGATTTCTCGCGTTATTGCAGCA
GCTACGGCGGCGTGAAGCGCGGCAATTTCTGGGGGCTGCCCGTCAATTTCCTCGCGTTCTCGCTCGTCACCGTGATCACG
ACGGCCGCGACGCTGCCGGTGTTCGGAGAACTGATCACCGATCCCGTCGAGACGGTCGGGCGCATCGATCATCCGAGCGC
CGTGATACTTGGCGCGCTGACCTTCACGATCGCGACGATCGGCATCAACATCGTCGCGAATTTCGTGTCGCCCGCGTTCG
ATTTCTCGAACGTCGCGCCGCGCCTGATCAGTTGGCGCGCGGGCGGGATGCTCGCGGCGGTCGCATCGGTGTTCATCACG
CCGTGGAATCTCTTCAACAATCCCGCGGTGATCCATTACACGCTCGACGTGCTCGGCAGCTTCATCGGGCCGCTGTACGG
CGTGCTGATCGTCGATTTCTATCTCGTGAAGCGCGGCGCGCTGCGGCGCGACGATCTGTACACGACGTCGGCCGACGGCG
CGTACTGGTATCGCGACGGCGTGAACCGGCGCGCGATCGCCGCGCTGTTGCCCGCGGCCGCGATCGCCGTCGCATGCGTG
ATGGCGCCCGCGCTGTCCGGGCTCGCGAATTTCTCGTGGTTCATCGGCGCGGCGCTCGGCGGCGCGTTCTATCGCGCGCT
CGCGAAAGCATGA

Upstream 100 bases:

>100_bases
CGCCGAACGCGCTCACTGAACTGACGAAATGCCGGGCGGCCGCGTGTGCGGACGCCTCGCCAGGGCGGCATCGGGCGATC
CCGTTCGAGCCGCGATTTCA

Downstream 100 bases:

>100_bases
CGGAGTCCCCATGAAAATAAAGCTGATCAATCCGAACACGACGCGGCGGATGACCGACGCGATGGGCCGTTGCGCGCGCG
AGGTCGCGTCGGCGGGCACG

Product: NCS1 nucleoside transporter family protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTIARLVRGRQGDVMAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSV
GGYVFAGSLFALGLTSWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQT
YLASSALVIVVLKFFPHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYM
VWRAGWRNIGLNLGGVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVIT
TAATLPVFGELITDPVETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFIT
PWNLFNNPAVIHYTLDVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACV
MAPALSGLANFSWFIGAALGGAFYRALAKA

Sequences:

>Translated_510_residues
MTIARLVRGRQGDVMAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSV
GGYVFAGSLFALGLTSWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQT
YLASSALVIVVLKFFPHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYM
VWRAGWRNIGLNLGGVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVIT
TAATLPVFGELITDPVETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFIT
PWNLFNNPAVIHYTLDVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACV
MAPALSGLANFSWFIGAALGGAFYRALAKA
>Mature_509_residues
TIARLVRGRQGDVMAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSVG
GYVFAGSLFALGLTSWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTY
LASSALVIVVLKFFPHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYMV
WRAGWRNIGLNLGGVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVITT
AATLPVFGELITDPVETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITP
WNLFNNPAVIHYTLDVLGSFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACVM
APALSGLANFSWFIGAALGGAFYRALAKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=471, Percent_Identity=28.6624203821656, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319495, Length=485, Percent_Identity=25.5670103092783, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6322219, Length=455, Percent_Identity=23.7362637362637, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319429, Length=476, Percent_Identity=22.8991596638655, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323266, Length=472, Percent_Identity=23.0932203389831, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324766, Length=472, Percent_Identity=22.2457627118644, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=20.9401709401709, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 55041; Mature: 54910

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIARLVRGRQGDVMAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLA
CHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
SQRWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQVLVALIVGISIVNVLCNLIARP
HCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPHWMP
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHH
VWRAGWRNIGLNLGGVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKR
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
GNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVETVGRIDHPSAVILGALTFTIATI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLDVLGS
HHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCEEEEEEHHHHHH
FIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACV
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
MAPALSGLANFSWFIGAALGGAFYRALAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]