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The map label for this gene is mhpE [C]

Identifier: 126445045

GI number: 126445045

Start: 2493787

End: 2494830

Strand: Reverse

Name: mhpE [C]

Synonym: BURPS668_A2590

Alternate gene names: 126445045

Gene position: 2494830-2493787 (Counterclockwise)

Preceding gene: 126442464

Following gene: 126444593

Centisome position: 79.77

GC content: 67.24

Gene sequence:

>1044_bases
ATGATACTGATCAGCGATGCGACCTTGCGCGACGGCAACCATGCGATTCGTCACCAACTGAGCGCCGCGCAGATACATGC
GTATGCGCGCGCGGCCGACGAAGCCGGCATCGATGTCGTCGAAGTCGGCCACGGCAATGGTCTCGGAGGCTCGTCTTGCC
TGCTCGGGCAGACGCCGATCGGCGATCGCCTGATGCTCGAGACCGCGCGCGCCGCGCTGCGCACGAGCCGGCTCGGCGTG
CATTTCATTCCGGGGCTCGGCAAGGCGGCGGACATCTCGCTTGCGCTCGAGATCGGCGTCGATGTCGTGCGCGTCGCGAC
GCATTGCACCGAGGCGAACGTGTCGGCGCGCTTCATCGAGCAGACCCGGACGGCCGGACGCACGGCGTTCGGCGTGCTGA
TGATGTCGCACATGGCGCCGCCCGATACGCTGCTCGCGCAGGCGAAGCTGATGGAGCGCTACGGCGCGCAGGCAGTGGTG
CTGATGGACAGCGCCGGGTATTCGACGCCGTCGCTCGTGCGCGCGAAGGTCGAGCGCCTCGTCGACGGTCTCGACATCGA
CGTCGGCTTTCACGCGCACAACAACCTCGGGCTCGCGGTCGCGAACAGCCTCGTCGCGCTCGAAGCGGGGGCGCGCATCG
TCGACGCATGCGTGAAAGGCTTCGGGGCCGGCGCGGGCAATACGCAGCTCGAAACGCTCGTCGCCGCGATGGAGCGCGAA
GGGCACGACACGCGCACGACGTTCGAGCGCGTGATGACGCTCGCGCGCGGCACGGAGACGTTTCTCAATCCGAAGACGCC
GCACATCCAGCCGGCGAACATCGCGAGCGGGCTGTACGGCCTTTTCTCCGGCTACGTGCCGCATATCCAGAAAGCCGCGC
AGGAATTCGGCGTCAACGAATTCGAGTTGTACAAGCGGCTTGCGGAGCGCAAGCTCGTCGCCGGGCAGGAGGACATCATC
ATCGAAGAGGCAAGCCGTCTCGCGCGCGAACGGGATGTGCAGCGGGCAACGGGCGGCGTGCGCGTTCGCGAGCTGTCCGC
GTGA

Upstream 100 bases:

>100_bases
ATTATCTGCCCGAGTACGCGGGCAATCTGGACATCATCAACTGCGCGGCGCTCGCGGTCGCCTCGCATCGGCACGCAACC
GCCAGACTAGGAGCCACACA

Downstream 100 bases:

>100_bases
GCGCGCGCTTTTCACACGATCGAGCGAGGAGGAAATCGTGACAGGTTTGAATGAACGAGGCTTTGGCGCATGGAATCGGA
TCGAGCGCGAAACGCTGACC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MILISDATLRDGNHAIRHQLSAAQIHAYARAADEAGIDVVEVGHGNGLGGSSCLLGQTPIGDRLMLETARAALRTSRLGV
HFIPGLGKAADISLALEIGVDVVRVATHCTEANVSARFIEQTRTAGRTAFGVLMMSHMAPPDTLLAQAKLMERYGAQAVV
LMDSAGYSTPSLVRAKVERLVDGLDIDVGFHAHNNLGLAVANSLVALEAGARIVDACVKGFGAGAGNTQLETLVAAMERE
GHDTRTTFERVMTLARGTETFLNPKTPHIQPANIASGLYGLFSGYVPHIQKAAQEFGVNEFELYKRLAERKLVAGQEDII
IEEASRLARERDVQRATGGVRVRELSA

Sequences:

>Translated_347_residues
MILISDATLRDGNHAIRHQLSAAQIHAYARAADEAGIDVVEVGHGNGLGGSSCLLGQTPIGDRLMLETARAALRTSRLGV
HFIPGLGKAADISLALEIGVDVVRVATHCTEANVSARFIEQTRTAGRTAFGVLMMSHMAPPDTLLAQAKLMERYGAQAVV
LMDSAGYSTPSLVRAKVERLVDGLDIDVGFHAHNNLGLAVANSLVALEAGARIVDACVKGFGAGAGNTQLETLVAAMERE
GHDTRTTFERVMTLARGTETFLNPKTPHIQPANIASGLYGLFSGYVPHIQKAAQEFGVNEFELYKRLAERKLVAGQEDII
IEEASRLARERDVQRATGGVRVRELSA
>Mature_347_residues
MILISDATLRDGNHAIRHQLSAAQIHAYARAADEAGIDVVEVGHGNGLGGSSCLLGQTPIGDRLMLETARAALRTSRLGV
HFIPGLGKAADISLALEIGVDVVRVATHCTEANVSARFIEQTRTAGRTAFGVLMMSHMAPPDTLLAQAKLMERYGAQAVV
LMDSAGYSTPSLVRAKVERLVDGLDIDVGFHAHNNLGLAVANSLVALEAGARIVDACVKGFGAGAGNTQLETLVAAMERE
GHDTRTTFERVMTLARGTETFLNPKTPHIQPANIASGLYGLFSGYVPHIQKAAQEFGVNEFELYKRLAERKLVAGQEDII
IEEASRLARERDVQRATGGVRVRELSA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=332, Percent_Identity=39.4578313253012, Blast_Score=214, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6320071, Length=248, Percent_Identity=27.0161290322581, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320019, Length=248, Percent_Identity=26.6129032258064, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_BURP0 (A3P825)

Other databases:

- EMBL:   CP000573
- RefSeq:   YP_001076485.1
- ProteinModelPortal:   A3P825
- SMR:   A3P825
- STRING:   A3P825
- GeneID:   4903937
- GenomeReviews:   CP000573_GR
- KEGG:   bpl:BURPS1106A_A2454
- TIGR:   BURPS1106A_A2454
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   MVPRHAG
- ProtClustDB:   PRK08195
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 37037; Mature: 37037

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 164-164 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILISDATLRDGNHAIRHQLSAAQIHAYARAADEAGIDVVEVGHGNGLGGSSCLLGQTPI
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCC
GDRLMLETARAALRTSRLGVHFIPGLGKAADISLALEIGVDVVRVATHCTEANVSARFIE
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHH
QTRTAGRTAFGVLMMSHMAPPDTLLAQAKLMERYGAQAVVLMDSAGYSTPSLVRAKVERL
HHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
VDGLDIDVGFHAHNNLGLAVANSLVALEAGARIVDACVKGFGAGAGNTQLETLVAAMERE
HCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GHDTRTTFERVMTLARGTETFLNPKTPHIQPANIASGLYGLFSGYVPHIQKAAQEFGVNE
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FELYKRLAERKLVAGQEDIIIEEASRLARERDVQRATGGVRVRELSA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure
MILISDATLRDGNHAIRHQLSAAQIHAYARAADEAGIDVVEVGHGNGLGGSSCLLGQTPI
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCC
GDRLMLETARAALRTSRLGVHFIPGLGKAADISLALEIGVDVVRVATHCTEANVSARFIE
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHH
QTRTAGRTAFGVLMMSHMAPPDTLLAQAKLMERYGAQAVVLMDSAGYSTPSLVRAKVERL
HHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
VDGLDIDVGFHAHNNLGLAVANSLVALEAGARIVDACVKGFGAGAGNTQLETLVAAMERE
HCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GHDTRTTFERVMTLARGTETFLNPKTPHIQPANIASGLYGLFSGYVPHIQKAAQEFGVNE
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FELYKRLAERKLVAGQEDIIIEEASRLARERDVQRATGGVRVRELSA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA