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The map label for this gene is potH [H]

Identifier: 126443933

GI number: 126443933

Start: 689538

End: 690518

Strand: Reverse

Name: potH [H]

Synonym: BURPS668_A0724

Alternate gene names: 126443933

Gene position: 690518-689538 (Counterclockwise)

Preceding gene: 126443131

Following gene: 126445140

Centisome position: 22.08

GC content: 65.95

Gene sequence:

>981_bases
ATGAGAACCTCTCCCTCCTCCCCGCTCGCGGCGGCCGCGCCCGCCGCACGCAGCGCGTTCTCCGCGCGCGCGCGCGCCGC
GCGCGTCGCGGCGCGCTTCACGCCGTCCGGGCGCAGCGTCGCGATCGGCGTGCCGTTCCTCTGGCTTGCGCTGTTCTTCG
CGTTGCCGTTCGTGCTCGTGCTGAAGATCAGCTTCGCCGATCAGGTGATGGGCATCCCGCCGTACACGGCGCTGATGGAC
GTCAAGGACGGCGCCGTGCGGTTCGCGCTGCAGCTCGGCCATTACGCGTTCCTGCTGCAGGACAGCCTCTATGTCGCGAC
CTACGTCAGCTCGCTGAAGATGGCCGCGGTGTCGACGCTCCTGTGCCTGCTGATCGGCTACCCGATGGCGTACTACATCG
CGCGCTGCGCGCCCGCGACGCGCAACCTGCTGATGATGGGCGTGATGCTGCCGTTCTGGACGTCGTTCCTGATTCGCGTC
TATGCATGGATCGGCATCCTGAAGGACGACGGCCTGCTGAACCACGCGCTGATCGCGATCGGCCTGATCCATTCGCCGCT
GCGCCTCTATCACACCGACGCGGGCGTCTACATCGGAATGGTCTACTCGTACCTGCCGTTCATGGTGATGCCGCTCTATG
CGCACCTCGTGAAGATGGACCTCACGCTGCTGGAGGCCGCCTATGACCTCGGCGCGAAGCCGTGGGTCGCGTTCACGCGG
ATCACGCTGCCGCTGTCGAAGAACGGCGTCATCGCGGGCAGCCTGCTCGTGTTCATCCCCGCCGTCGGCGAATACGTGAT
CCCCGAGCTGCTCGGCGGCGCCGATACGCTGATGATCGGCCGCGTGATGTGGGACGAGTTCTTCAACAACATGGACTGGC
CGATGGCCTCCGCGGTGACGGTCGCGATGGTGCTGCTGCTCCTCGTGCCGATGGCCGTGTTCCAGTACTACCAGGTGAAG
GAACTGGAGGACGCGAAATGA

Upstream 100 bases:

>100_bases
CCAATCTCTCGAGCTCGCACCTGATGCAGGACGGTGCGCCCGCGTACAACGACGACGTGTTCGTCTCCTGGTCGCCCGCA
AGCGGCGTGGTGCTGACGCA

Downstream 100 bases:

>100_bases
TCAAGCCCAACAAGCCGCTTTGCGCCGGCGTGCTGACGCTCGGCTTCCTGTTTCTCTACATCCCGATCGTGAGCCTCGTC
GTCTATTCGTTTAACGAATC

Product: putrescine ABC transporter permease PotH

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRTSPSSPLAAAAPAARSAFSARARAARVAARFTPSGRSVAIGVPFLWLALFFALPFVLVLKISFADQVMGIPPYTALMD
VKDGAVRFALQLGHYAFLLQDSLYVATYVSSLKMAAVSTLLCLLIGYPMAYYIARCAPATRNLLMMGVMLPFWTSFLIRV
YAWIGILKDDGLLNHALIAIGLIHSPLRLYHTDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAFTR
ITLPLSKNGVIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMAVFQYYQVK
ELEDAK

Sequences:

>Translated_326_residues
MRTSPSSPLAAAAPAARSAFSARARAARVAARFTPSGRSVAIGVPFLWLALFFALPFVLVLKISFADQVMGIPPYTALMD
VKDGAVRFALQLGHYAFLLQDSLYVATYVSSLKMAAVSTLLCLLIGYPMAYYIARCAPATRNLLMMGVMLPFWTSFLIRV
YAWIGILKDDGLLNHALIAIGLIHSPLRLYHTDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAFTR
ITLPLSKNGVIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMAVFQYYQVK
ELEDAK
>Mature_326_residues
MRTSPSSPLAAAAPAARSAFSARARAARVAARFTPSGRSVAIGVPFLWLALFFALPFVLVLKISFADQVMGIPPYTALMD
VKDGAVRFALQLGHYAFLLQDSLYVATYVSSLKMAAVSTLLCLLIGYPMAYYIARCAPATRNLLMMGVMLPFWTSFLIRV
YAWIGILKDDGLLNHALIAIGLIHSPLRLYHTDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAFTR
ITLPLSKNGVIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMAVFQYYQVK
ELEDAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=288, Percent_Identity=55.2083333333333, Blast_Score=317, Evalue=5e-88,
Organism=Escherichia coli, GI1787369, Length=285, Percent_Identity=40.3508771929825, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=30.9859154929577, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35824; Mature: 35824

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSPSSPLAAAAPAARSAFSARARAARVAARFTPSGRSVAIGVPFLWLALFFALPFVLV
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHH
LKISFADQVMGIPPYTALMDVKDGAVRFALQLGHYAFLLQDSLYVATYVSSLKMAAVSTL
HHHHHHHHHCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCLLIGYPMAYYIARCAPATRNLLMMGVMLPFWTSFLIRVYAWIGILKDDGLLNHALIAI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GLIHSPLRLYHTDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAFTR
HHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITLPLSKNGVIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
VAMVLLLLVPMAVFQYYQVKELEDAK
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTSPSSPLAAAAPAARSAFSARARAARVAARFTPSGRSVAIGVPFLWLALFFALPFVLV
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHH
LKISFADQVMGIPPYTALMDVKDGAVRFALQLGHYAFLLQDSLYVATYVSSLKMAAVSTL
HHHHHHHHHCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCLLIGYPMAYYIARCAPATRNLLMMGVMLPFWTSFLIRVYAWIGILKDDGLLNHALIAI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GLIHSPLRLYHTDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAFTR
HHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITLPLSKNGVIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
VAMVLLLLVPMAVFQYYQVKELEDAK
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]