Click here to switch to the map view.

The map label for this gene is potI [H]

Identifier: 126445140

GI number: 126445140

Start: 688612

End: 689541

Strand: Reverse

Name: potI [H]

Synonym: BURPS668_A0723

Alternate gene names: 126445140

Gene position: 689541-688612 (Counterclockwise)

Preceding gene: 126443933

Following gene: 126444089

Centisome position: 22.05

GC content: 66.88

Gene sequence:

>930_bases
ATGATCAAGCCCAACAAGCCGCTTTGCGCCGGCGTGCTGACGCTCGGCTTCCTGTTTCTCTACATCCCGATCGTGAGCCT
CGTCGTCTATTCGTTTAACGAATCGAAGCTCGTCACGGTGTGGTCAGGCTTCTCGCTGAAGTGGTACGCGGCGCTCTGGC
GGGACGACGAACTGCTGACGAGCGCGTGGCTATCGCTGAAGATCGGCCTGCTGACCGCGTTCGCGTCGGTGTTCATCGGC
ACGTGGGCAGGCTTCGTGCTCGCGCGCTTCGGACGCTTTCGCGGCTTCACGCTGTACACCGGCATGATCAACGCGCCGCT
CGTGATTCCCGAAGTGATTCAGGGCATCTCGCTGTTGCTGCTGTTCGTCGCGCTCGAGCAGATGGCCGGCTGGCCGAAGG
GGCGCGGGATGGTGACGATCTGGATCGGCCACGTGATGCTGTGCGTGTCGTACGTCGCGATCATCGTGCAGTCGCGCGTG
AAGGAGCTGAACAAGGCGCTCGAGGAAGCGGCGCTCGACCTCGGCGCGACGCCGCTGAAGGTGTTCTTCGTGATCACGCT
GCCGCTGATCTCGCAGGCGCTGCTCTCCGGCTGGCTGCTGTCGTTCACGCTGTCGATCGACGATCTCGTGCTCTCCGCGT
TCCTCTCCGGCCCCGGCTCGACGACGCTGCCGCTCGTCGTGTTCTCGCGCGTGCGCCTCGGCCTGAACCCCGAAATGAAC
GCGCTCGCGACGCTCTTCATCACGGCGGTGACGATCGGCGTGATCGCCGTCAACCAGATGATGCTGAGCCGCGAACGCCG
GCGCGAACGCGACATGCGCCAGGCGTTCGCGCCGCCCGCGCCGTGCGATTCGCCCGAATCCGGCCCGGCCGGCGGCGCGC
CGCGCGCGGCGCGCGCCGCCGCGACGCGCAAGCCGCTCGGCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCCTCCGCGGTGACGGTCGCGATGGTGCTGCTGCTCCTCGTGCCGATGGCCGTGTTCCAGTACTACCAGGTG
AAGGAACTGGAGGACGCGAA

Downstream 100 bases:

>100_bases
GCGCGCTGCGCCCGCCCGAACGCACATCGAATCGAAGTCCTTCGCAATACGACACCCAGGAGAATCGCATGAAGAAGAAG
CTCATCTGTCTGCTCGTGGC

Product: putrescine ABC transporter permease PotI

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MIKPNKPLCAGVLTLGFLFLYIPIVSLVVYSFNESKLVTVWSGFSLKWYAALWRDDELLTSAWLSLKIGLLTAFASVFIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMAGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KELNKALEEAALDLGATPLKVFFVITLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIAVNQMMLSRERRRERDMRQAFAPPAPCDSPESGPAGGAPRAARAAATRKPLGAA

Sequences:

>Translated_309_residues
MIKPNKPLCAGVLTLGFLFLYIPIVSLVVYSFNESKLVTVWSGFSLKWYAALWRDDELLTSAWLSLKIGLLTAFASVFIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMAGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KELNKALEEAALDLGATPLKVFFVITLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIAVNQMMLSRERRRERDMRQAFAPPAPCDSPESGPAGGAPRAARAAATRKPLGAA
>Mature_309_residues
MIKPNKPLCAGVLTLGFLFLYIPIVSLVVYSFNESKLVTVWSGFSLKWYAALWRDDELLTSAWLSLKIGLLTAFASVFIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMAGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KELNKALEEAALDLGATPLKVFFVITLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIAVNQMMLSRERRRERDMRQAFAPPAPCDSPESGPAGGAPRAARAAATRKPLGAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=57.9545454545455, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1787368, Length=241, Percent_Identity=37.344398340249, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787714, Length=255, Percent_Identity=30.1960784313725, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788764, Length=217, Percent_Identity=30.8755760368664, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33545; Mature: 33545

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPNKPLCAGVLTLGFLFLYIPIVSLVVYSFNESKLVTVWSGFSLKWYAALWRDDELLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHH
SAWLSLKIGLLTAFASVFIGTWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMAGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKELNKALEEAALDLGATPLK
HHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVITLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIAVNQMMLSRERRRERDMRQAFAPPAPCDSPESGPAGGAPRAARAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
ATRKPLGAA
HHCCCCCCC
>Mature Secondary Structure
MIKPNKPLCAGVLTLGFLFLYIPIVSLVVYSFNESKLVTVWSGFSLKWYAALWRDDELLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHH
SAWLSLKIGLLTAFASVFIGTWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMAGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKELNKALEEAALDLGATPLK
HHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVITLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIAVNQMMLSRERRRERDMRQAFAPPAPCDSPESGPAGGAPRAARAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
ATRKPLGAA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]