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The map label for this gene is pksF [H]

Identifier: 126443682

GI number: 126443682

Start: 1366131

End: 1367375

Strand: Reverse

Name: pksF [H]

Synonym: BURPS668_A1466

Alternate gene names: 126443682

Gene position: 1367375-1366131 (Counterclockwise)

Preceding gene: 126444728

Following gene: 126442784

Centisome position: 43.72

GC content: 70.76

Gene sequence:

>1245_bases
ATGCGAAGCTCTGAGCGCGACGAAATCCTCGTCACCGGCGTCGGCGTCGTATCGGCCATCGGCCAGGGCAGGCAGCCGTT
CATCGACGCGCTGCTCGACGGCCGCCACGCATTCGGCGTGATGCGCCGGCCGGGCCGGCAGTTGCCGGACGACGCGGGGG
CCGGCCCGGCGCCCGCGTTCTTCGGCGCGGAAATCGACTCGATCGCGTTGCCGCCCATGCTGGACGCGCACCGGCGCACC
GCGTCGTATTCCGCCCAGGCGGCCGTCGCATGCGTGCGCGAGGCGTGGGACGACGCCGCGCTCGGCGACGTCGATCCGCT
GCGCATCGGCCTCGTGGTCGGCGGCTCGAACGTTCAGCAGCGCGAACAAGCGCTCGTTCACGACACCTACCGGGGCCGGT
ACGCGTTCCTGCGCCCGAGCTACGGCCTGTCGTTCATGGATAGCGATCTCTGCGGCCTGTGCACCGAACTGTTCGGCATT
CGCGGCGTCGCGTTCACCGTCGGCGGCGCGTCGGCGAGCGGCCAGCTCGCCGTGCTGCAGGCATGCCGTCTCGTCGAATC
GGGCAGCGTCGACGCGTGCATCGCGGTGGGCGCGCTGATGGATCTGTCGTACTGGGAGTGCCAGGCGTTGCGCTCGCTCG
GCGCGATGGGCTCGGATCGCTTCGCGGACGAGCCCGACGCCGCATGCCGCCCGTTCGATCAAGACCGGGACGGCTTCATC
TACGGCGAATGCAGCGGCGCGATCGTCGTGGAGCGCGCGGCACGCATGCGCCGCGCGCACGTCTCGCCGTACGCGAAATG
GCTCGGCGGCTGCATCCGGATGGACGGCACGCGCGAGCCCGCGCCGTCGCTCGACGGCGAGATGGCCGTGATCGAAGGCG
CGCTGCGCGACGCCGGCATCGGTGCGGACAGCATCGATTACGTGAATCCGCACGGCAGCGGCTCGACGCTCGGCGACGCG
ACCGAGCTCGACGCGCTCGGCCGCAGCGGCCTCGCGCACGCGCGCATCAACACGACGAAGTCGCTCGTCGGGCACGGCCT
GAGCGCGGCGGGCATCGTCGAGCTGATCGCGACGCTGCTGCAAATGAGGGCGCGGCGCCTGCATCCGAGCCGCAATCTCG
ACCGGCCGATCGACGAGCGGTTCGACTGGGTGCGTTCGACCTGCGGGTCGGACATCCGGCGCGCCCTGAAATTGAGCATC
GGATTCGGCGGCATCAACACCGCGCTCTGTCTCGAGCGCTGTTGA

Upstream 100 bases:

>100_bases
TCGGAAATCGTCATCATGACGCTCGAATCGCTTTCGCTGCGCATTCCGCTCGTCGAGTTCGCCGGCGCGCGCAACATCGG
CGAGCTGGCGGAAGTGCTGT

Downstream 100 bases:

>100_bases
CGCTCGGCCGGAAACACGCTGTAGGCATGGGAAGCAGGAACATGGGCGACTATCTGGCGCAGGACGCGCGTATCGAGCGG
ATCACGGTTCGCGATCCCGA

Product: polyketide beta-ketoacyl:acyl carrier protein synthase

Products: NA

Alternate protein names: Malonyl acyl-carrier-protein decarboxylase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRSSERDEILVTGVGVVSAIGQGRQPFIDALLDGRHAFGVMRRPGRQLPDDAGAGPAPAFFGAEIDSIALPPMLDAHRRT
ASYSAQAAVACVREAWDDAALGDVDPLRIGLVVGGSNVQQREQALVHDTYRGRYAFLRPSYGLSFMDSDLCGLCTELFGI
RGVAFTVGGASASGQLAVLQACRLVESGSVDACIAVGALMDLSYWECQALRSLGAMGSDRFADEPDAACRPFDQDRDGFI
YGECSGAIVVERAARMRRAHVSPYAKWLGGCIRMDGTREPAPSLDGEMAVIEGALRDAGIGADSIDYVNPHGSGSTLGDA
TELDALGRSGLAHARINTTKSLVGHGLSAAGIVELIATLLQMRARRLHPSRNLDRPIDERFDWVRSTCGSDIRRALKLSI
GFGGINTALCLERC

Sequences:

>Translated_414_residues
MRSSERDEILVTGVGVVSAIGQGRQPFIDALLDGRHAFGVMRRPGRQLPDDAGAGPAPAFFGAEIDSIALPPMLDAHRRT
ASYSAQAAVACVREAWDDAALGDVDPLRIGLVVGGSNVQQREQALVHDTYRGRYAFLRPSYGLSFMDSDLCGLCTELFGI
RGVAFTVGGASASGQLAVLQACRLVESGSVDACIAVGALMDLSYWECQALRSLGAMGSDRFADEPDAACRPFDQDRDGFI
YGECSGAIVVERAARMRRAHVSPYAKWLGGCIRMDGTREPAPSLDGEMAVIEGALRDAGIGADSIDYVNPHGSGSTLGDA
TELDALGRSGLAHARINTTKSLVGHGLSAAGIVELIATLLQMRARRLHPSRNLDRPIDERFDWVRSTCGSDIRRALKLSI
GFGGINTALCLERC
>Mature_414_residues
MRSSERDEILVTGVGVVSAIGQGRQPFIDALLDGRHAFGVMRRPGRQLPDDAGAGPAPAFFGAEIDSIALPPMLDAHRRT
ASYSAQAAVACVREAWDDAALGDVDPLRIGLVVGGSNVQQREQALVHDTYRGRYAFLRPSYGLSFMDSDLCGLCTELFGI
RGVAFTVGGASASGQLAVLQACRLVESGSVDACIAVGALMDLSYWECQALRSLGAMGSDRFADEPDAACRPFDQDRDGFI
YGECSGAIVVERAARMRRAHVSPYAKWLGGCIRMDGTREPAPSLDGEMAVIEGALRDAGIGADSIDYVNPHGSGSTLGDA
TELDALGRSGLAHARINTTKSLVGHGLSAAGIVELIATLLQMRARRLHPSRNLDRPIDERFDWVRSTCGSDIRRALKLSI
GFGGINTALCLERC

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It decarboxylates selectively the malonyl group attached on the acyl-carrier-protein AcpK (Mal-AcpK) [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=432, Percent_Identity=27.5462962962963, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI41872631, Length=269, Percent_Identity=26.7657992565056, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI223972627, Length=140, Percent_Identity=35, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788663, Length=423, Percent_Identity=28.8416075650118, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787337, Length=310, Percent_Identity=31.6129032258064, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212642053, Length=269, Percent_Identity=27.5092936802974, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983541, Length=434, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=276, Percent_Identity=26.8115942028986, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983535, Length=227, Percent_Identity=31.7180616740088, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320904, Length=445, Percent_Identity=25.3932584269663, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24644341, Length=432, Percent_Identity=29.3981481481481, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI221330659, Length=343, Percent_Identity=23.0320699708455, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920632, Length=343, Percent_Identity=23.0320699708455, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581345, Length=278, Percent_Identity=25.1798561151079, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: =4.1.1.87 [H]

Molecular weight: Translated: 44054; Mature: 44054

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSERDEILVTGVGVVSAIGQGRQPFIDALLDGRHAFGVMRRPGRQLPDDAGAGPAPAF
CCCCCCCCEEEECHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FGAEIDSIALPPMLDAHRRTASYSAQAAVACVREAWDDAALGDVDPLRIGLVVGGSNVQQ
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHH
REQALVHDTYRGRYAFLRPSYGLSFMDSDLCGLCTELFGIRGVAFTVGGASASGQLAVLQ
HHHHHHHHHCCCCEEEECCCCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
ACRLVESGSVDACIAVGALMDLSYWECQALRSLGAMGSDRFADEPDAACRPFDQDRDGFI
HHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCEE
YGECSGAIVVERAARMRRAHVSPYAKWLGGCIRMDGTREPAPSLDGEMAVIEGALRDAGI
EECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GADSIDYVNPHGSGSTLGDATELDALGRSGLAHARINTTKSLVGHGLSAAGIVELIATLL
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHH
QMRARRLHPSRNLDRPIDERFDWVRSTCGSDIRRALKLSIGFGGINTALCLERC
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRSSERDEILVTGVGVVSAIGQGRQPFIDALLDGRHAFGVMRRPGRQLPDDAGAGPAPAF
CCCCCCCCEEEECHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FGAEIDSIALPPMLDAHRRTASYSAQAAVACVREAWDDAALGDVDPLRIGLVVGGSNVQQ
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHH
REQALVHDTYRGRYAFLRPSYGLSFMDSDLCGLCTELFGIRGVAFTVGGASASGQLAVLQ
HHHHHHHHHCCCCEEEECCCCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
ACRLVESGSVDACIAVGALMDLSYWECQALRSLGAMGSDRFADEPDAACRPFDQDRDGFI
HHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCEE
YGECSGAIVVERAARMRRAHVSPYAKWLGGCIRMDGTREPAPSLDGEMAVIEGALRDAGI
EECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GADSIDYVNPHGSGSTLGDATELDALGRSGLAHARINTTKSLVGHGLSAAGIVELIATLL
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHH
QMRARRLHPSRNLDRPIDERFDWVRSTCGSDIRRALKLSIGFGGINTALCLERC
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]