Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 126443625

GI number: 126443625

Start: 242294

End: 243295

Strand: Reverse

Name: gcvA [H]

Synonym: BURPS668_A0274

Alternate gene names: 126443625

Gene position: 243295-242294 (Counterclockwise)

Preceding gene: 126444744

Following gene: 126445085

Centisome position: 7.78

GC content: 71.96

Gene sequence:

>1002_bases
ATGCGCGCCACGCGCGGCGCGAGCAGGCGCAACGGAAGCAGCCAAGATAGCGGCGCGCAGATGTCCGAACAATCGAAAAA
TTCGCAAGCCGGGCATGCGCCGCTTGCATACCCGCTCGCCGCCCTCACGCGCGCGCTGCCGACGCTGGCCGCGCTCCAGT
CGTTCGTCGCGGCCGCGCAGCTCGGCAGTCTCAGCAAGGCCGCCGCACATCTATGCCGCACGCAGGGCGCGGTGAGCCGC
CAGATTCAGCAGCTCGAGGCCCACTATCGATGCGCGCTGTTCGTCCGCCAGCCGACCGGACTGACGCTCACCGCCGACGG
CGACGCGCTCTTCGCCGTGGCGGCCGACGTGCTCGCGCGGCTCGTTCGGCACGCGCGCGAGCGCGACGAAGCGGCGGCGG
CCGTCACCGTGCGCGTGCCCTCGACATTCGCGATTCGATGGCTGCTGCCGCGCCTGCCGGCGATTCGGCGGGCGCTCGGC
GCGACGCAATTGTGCCTGTCGACGTCGGCGAACGACACGCCGGATTTCTCCGAGCCGGACATCGATGCGATCGTCGCCCG
CGGCGACGGGCGCTGGCCCGGCGTCGAAGCGATTCCGCTGTTCGCGGAAACGCTCGCGCCGATGTGCGGCCCCGCCCTCG
CCGCGTCGCTGAAATCGGTCGACGATCTCGCGCACGTCACGCTGCTTCATCCAGGGCGCGGCCGCGCGGAATGGCGCTGC
TGGCTCGACGCGGTCGGCGCCGCGCACATCGACGCGACGCGCGGCCCGGTATTCGACACGCTCGAGCTGACGCTGAGCGC
GGCGGCGGAAGGACACGGCGTCGCGATCGGCGATCCGCGCATGGCGGGCGACCGGCTCCGCGCGGGCACGCTGACGATGC
CGCTGCGCGAGACCGCGGCGAACGGCCTGTCGTACTACCTCGTGTATCCGGCGCAGCGCGCGACGCAGCCGAAGATTCGC
GCGCTGGCGAACGTGCTCGTCAAACTCGCGCAAGCGCGCTGA

Upstream 100 bases:

>100_bases
CGTCGCCGCGCTCGAGCGCCTTCACGTATGCGTGAATCTGCCGCGTGTATAGTGCATTCGATGACGAGTCCACGATCTTC
AATCCTCCGATCATTCGCCG

Downstream 100 bases:

>100_bases
GCACTCCGCGCGGCCCCGGCGCGGTCCGGGGCCGCCGCCGTCACGGCTTGACGCCTTCGATCACGCTGTTCATGCCTTGC
GTCGGGCGCACGGCCGTCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRATRGASRRNGSSQDSGAQMSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSR
QIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALG
ATQLCLSTSANDTPDFSEPDIDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRC
WLDAVGAAHIDATRGPVFDTLELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIR
ALANVLVKLAQAR

Sequences:

>Translated_333_residues
MRATRGASRRNGSSQDSGAQMSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSR
QIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALG
ATQLCLSTSANDTPDFSEPDIDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRC
WLDAVGAAHIDATRGPVFDTLELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIR
ALANVLVKLAQAR
>Mature_333_residues
MRATRGASRRNGSSQDSGAQMSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQLGSLSKAAAHLCRTQGAVSR
QIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLARLVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALG
ATQLCLSTSANDTPDFSEPDIDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRC
WLDAVGAAHIDATRGPVFDTLELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAANGLSYYLVYPAQRATQPKIR
ALANVLVKLAQAR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=34.2657342657343, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1786448, Length=275, Percent_Identity=30.1818181818182, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=27.719298245614, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693193, Length=277, Percent_Identity=29.6028880866426, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35256; Mature: 35256

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATRGASRRNGSSQDSGAQMSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQ
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSLSKAAAHLCRTQGAVSRQIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLAR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHH
LVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPD
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRC
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHH
WLDAVGAAHIDATRGPVFDTLELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAA
HHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEECCHHHHHC
NGLSYYLVYPAQRATQPKIRALANVLVKLAQAR
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRATRGASRRNGSSQDSGAQMSEQSKNSQAGHAPLAYPLAALTRALPTLAALQSFVAAAQ
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSLSKAAAHLCRTQGAVSRQIQQLEAHYRCALFVRQPTGLTLTADGDALFAVAADVLAR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHH
LVRHARERDEAAAAVTVRVPSTFAIRWLLPRLPAIRRALGATQLCLSTSANDTPDFSEPD
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IDAIVARGDGRWPGVEAIPLFAETLAPMCGPALAASLKSVDDLAHVTLLHPGRGRAEWRC
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHH
WLDAVGAAHIDATRGPVFDTLELTLSAAAEGHGVAIGDPRMAGDRLRAGTLTMPLRETAA
HHHHHCHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEECCHHHHHC
NGLSYYLVYPAQRATQPKIRALANVLVKLAQAR
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]