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The map label for this gene is cilB [H]

Identifier: 126442992

GI number: 126442992

Start: 2247310

End: 2248191

Strand: Reverse

Name: cilB [H]

Synonym: BURPS668_A2305

Alternate gene names: 126442992

Gene position: 2248191-2247310 (Counterclockwise)

Preceding gene: 126443570

Following gene: 126443557

Centisome position: 71.89

GC content: 71.77

Gene sequence:

>882_bases
ATGTCGATATCGATTCCGCACGACGCAGCCGCTCCGGCGGCATGCGATCGGCTCCACAGTGCGTTGCGCAGCTGGCTCTT
CACGCCGGCCACGCGCGCCGATCGCTTTTCAAACGCCTCCGAGTGCGGCGCTGACGTGCTCATCGTCGATCTGGAGGATT
CGGTCGCCCCGGCCCACAAGGCGTACGCCCGAGACCACGCGCGCGCGTGGCTCTCTAGCGGGCAGGCGCACGCGTGGCCG
GCTTCGGCCGTCCGCATCAACGCGCCCGATACGGTGGCGGGCCACGCGGACCTCGGGATGCTGATCGACGCGCCGCGCGC
GCCCGATTTCATCGTTGTGCCGAAAGTCGAAACGCAGCAGCGCATCGCGCATCTCGATGCGCTGTTGCGCGACGCGGGCC
GCCCGCCCCGATACGGCTTCGTCGCGATGATCGAATCCGCGGCGGGCGCCGATGCGGCCGACGCGATCGTGCGCGCGTGC
GGCTCGATCGGGGCGCTGATGTTCGGCGCGGCCGACTACGCGTCCGATGTCGCCGCACAACCCGATGCGCTCGCGCTGCA
GATCGCGCGCGTGCGGCTCGCCGCGGCCTGCGCGCGTGGCCGCATCGCCGCGATCGATGCGCCCTGCTTTGCATTGCGCG
ACGCGGCGGCCCTCGATGCCGAGCTTGCGTTTGCCGTGCGCAACGGCCTTCGCGGCAAGGCGGCGATTCACCCGGCGCAC
GTCGCACCGATCAATGCGGCATTCGCGCCTTCGCCGCAGCGGATCGCATGGGCGCGCCGCGTGATCGGCGCGGCGTCGCA
AGGCGCGGCCGTCGTCGACGGCCGCATGGTCGACGAAGCCGTCGCGCGCGAGGCGAGGGACGTTCTCGCCGCGGCGCGAT
GA

Upstream 100 bases:

>100_bases
CCGGTTCGCCGAAGGCTGCCCCCGAAAATGCCGCATTGTCCGGCGCGCGACGGGTGGCGACAATGCACTCGTCGTTTCGC
TCAACGAGGAGTGCCGGTTC

Downstream 100 bases:

>100_bases
CGGATACCCCGTTCTTTCTTCAACCAGGAGGCGAGCATGAGCACGACGATTTCCGCGTACCGACAGATCGGCGACAAGCG
GTTTCGCGAGATATCCGGCC

Product: hypothetical protein

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSISIPHDAAAPAACDRLHSALRSWLFTPATRADRFSNASECGADVLIVDLEDSVAPAHKAYARDHARAWLSSGQAHAWP
ASAVRINAPDTVAGHADLGMLIDAPRAPDFIVVPKVETQQRIAHLDALLRDAGRPPRYGFVAMIESAAGADAADAIVRAC
GSIGALMFGAADYASDVAAQPDALALQIARVRLAAACARGRIAAIDAPCFALRDAAALDAELAFAVRNGLRGKAAIHPAH
VAPINAAFAPSPQRIAWARRVIGAASQGAAVVDGRMVDEAVAREARDVLAAAR

Sequences:

>Translated_293_residues
MSISIPHDAAAPAACDRLHSALRSWLFTPATRADRFSNASECGADVLIVDLEDSVAPAHKAYARDHARAWLSSGQAHAWP
ASAVRINAPDTVAGHADLGMLIDAPRAPDFIVVPKVETQQRIAHLDALLRDAGRPPRYGFVAMIESAAGADAADAIVRAC
GSIGALMFGAADYASDVAAQPDALALQIARVRLAAACARGRIAAIDAPCFALRDAAALDAELAFAVRNGLRGKAAIHPAH
VAPINAAFAPSPQRIAWARRVIGAASQGAAVVDGRMVDEAVAREARDVLAAAR
>Mature_292_residues
SISIPHDAAAPAACDRLHSALRSWLFTPATRADRFSNASECGADVLIVDLEDSVAPAHKAYARDHARAWLSSGQAHAWPA
SAVRINAPDTVAGHADLGMLIDAPRAPDFIVVPKVETQQRIAHLDALLRDAGRPPRYGFVAMIESAAGADAADAIVRACG
SIGALMFGAADYASDVAAQPDALALQIARVRLAAACARGRIAAIDAPCFALRDAAALDAELAFAVRNGLRGKAAIHPAHV
APINAAFAPSPQRIAWARRVIGAASQGAAVVDGRMVDEAVAREARDVLAAAR

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=25.0847457627119, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI87081764, Length=286, Percent_Identity=28.6713286713287, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557458, Length=291, Percent_Identity=25.085910652921, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30445; Mature: 30314

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISIPHDAAAPAACDRLHSALRSWLFTPATRADRFSNASECGADVLIVDLEDSVAPAHK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCEEEEECCCCCCHHHH
AYARDHARAWLSSGQAHAWPASAVRINAPDTVAGHADLGMLIDAPRAPDFIVVPKVETQQ
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCEEEECCCHHHH
RIAHLDALLRDAGRPPRYGFVAMIESAAGADAADAIVRACGSIGALMFGAADYASDVAAQ
HHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
PDALALQIARVRLAAACARGRIAAIDAPCFALRDAAALDAELAFAVRNGLRGKAAIHPAH
CHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
VAPINAAFAPSPQRIAWARRVIGAASQGAAVVDGRMVDEAVAREARDVLAAAR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SISIPHDAAAPAACDRLHSALRSWLFTPATRADRFSNASECGADVLIVDLEDSVAPAHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCEEEEECCCCCCHHHH
AYARDHARAWLSSGQAHAWPASAVRINAPDTVAGHADLGMLIDAPRAPDFIVVPKVETQQ
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCEEEECCCHHHH
RIAHLDALLRDAGRPPRYGFVAMIESAAGADAADAIVRACGSIGALMFGAADYASDVAAQ
HHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
PDALALQIARVRLAAACARGRIAAIDAPCFALRDAAALDAELAFAVRNGLRGKAAIHPAH
CHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
VAPINAAFAPSPQRIAWARRVIGAASQGAAVVDGRMVDEAVAREARDVLAAAR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA