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The map label for this gene is spaP [H]

Identifier: 126442644

GI number: 126442644

Start: 2104066

End: 2104746

Strand: Reverse

Name: spaP [H]

Synonym: BURPS668_A2167

Alternate gene names: 126442644

Gene position: 2104746-2104066 (Counterclockwise)

Preceding gene: 126443367

Following gene: 126445290

Centisome position: 67.3

GC content: 62.85

Gene sequence:

>681_bases
ATGGCGAATAACGAAATCGCGCTGATCGTCCTGCTGACGGCGGCCACGCTCGTGCCGTTCGTCGTCGCGGCGGGCTCGTG
CTTCATCAAGTTCTCGATCGTGCTCGTGCTCGTGCGCAACGCGCTCGGCATCCAGCAGGTGCCGTCGAATCTCGCGCTCA
ACAGCATCGCGCTCATCATGTCGCTGTTCGTGATGATGCCCGTCGCGCAGAGCGCGTACCGATATCTGCAGCACCATCCG
CTCGACGTGATGAGCGGCGCGTCGGTCAACGAGTTCATCGACGGCGGGCTCGGCGATTACAAGCGCTATCTGACGCGCTA
CTCGGACCCGGAACTCGTGCGCTTCTTCGAGCGCGCGCAGACCGCGCGCCTGAAGGGCGACGACGCCGACGCCGCCGATG
CGGACGACGCGCCCGACGGCCTCGACAATTCGTTGTTCTCGCTGCTGCCCGCGTATGCGCTCACCGAGATCAAGAGCGCG
TTCAAGATCGGCTTCTACCTGTACCTGCCGTTTCTCGTCGTCGACATGGTGGTGTCGAGCGTGCTGCTCGCGCTCGGGAT
GATGATGATGAGCCCGGTGACGATCTCGGTGCCGATCAAGCTGATCCTGTTCGTCGCGATGGACGGCTGGACGCTGATCT
GCAAGGGCCTGATCGAGCAGTACCTGAACCTGATGCAATGA

Upstream 100 bases:

>100_bases
GCGAACGGCCAGCCGTTCGGCCGCGGCGAGCTGATCCAGGTCGGCGAGCAGCTCGCGGTCGAGGTGAAGGCGCTCTGGCG
CGCGAAGCCGGCGGCGTGCG

Downstream 100 bases:

>100_bases
CCGCGCCGACGCGCGCTTTTTTAGGAAGACAAACGATGGCTGAACTCACCTACGCGGGCGACAAGGCAATCCTGCTCGTG
ATCCTGCTGTGCGCGGCGCC

Product: surface presentation of antigens protein SpaP

Products: NA

Alternate protein names: Spa24 protein [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MANNEIALIVLLTAATLVPFVVAAGSCFIKFSIVLVLVRNALGIQQVPSNLALNSIALIMSLFVMMPVAQSAYRYLQHHP
LDVMSGASVNEFIDGGLGDYKRYLTRYSDPELVRFFERAQTARLKGDDADAADADDAPDGLDNSLFSLLPAYALTEIKSA
FKIGFYLYLPFLVVDMVVSSVLLALGMMMMSPVTISVPIKLILFVAMDGWTLICKGLIEQYLNLMQ

Sequences:

>Translated_226_residues
MANNEIALIVLLTAATLVPFVVAAGSCFIKFSIVLVLVRNALGIQQVPSNLALNSIALIMSLFVMMPVAQSAYRYLQHHP
LDVMSGASVNEFIDGGLGDYKRYLTRYSDPELVRFFERAQTARLKGDDADAADADDAPDGLDNSLFSLLPAYALTEIKSA
FKIGFYLYLPFLVVDMVVSSVLLALGMMMMSPVTISVPIKLILFVAMDGWTLICKGLIEQYLNLMQ
>Mature_225_residues
ANNEIALIVLLTAATLVPFVVAAGSCFIKFSIVLVLVRNALGIQQVPSNLALNSIALIMSLFVMMPVAQSAYRYLQHHPL
DVMSGASVNEFIDGGLGDYKRYLTRYSDPELVRFFERAQTARLKGDDADAADADDAPDGLDNSLFSLLPAYALTEIKSAF
KIGFYLYLPFLVVDMVVSSVLLALGMMMMSPVTISVPIKLILFVAMDGWTLICKGLIEQYLNLMQ

Specific function: Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins [H]

COG id: COG4790

COG function: function code U; Type III secretory pathway, component EscR

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliP/mopC/spaP family [H]

Homologues:

Organism=Escherichia coli, GI1788259, Length=214, Percent_Identity=35.0467289719626, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005838
- InterPro:   IPR005773 [H]

Pfam domain/function: PF00813 FliP [H]

EC number: NA

Molecular weight: Translated: 24770; Mature: 24638

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01060 FLIP_1 ; PS01061 FLIP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNEIALIVLLTAATLVPFVVAAGSCFIKFSIVLVLVRNALGIQQVPSNLALNSIALIM
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SLFVMMPVAQSAYRYLQHHPLDVMSGASVNEFIDGGLGDYKRYLTRYSDPELVRFFERAQ
HHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
TARLKGDDADAADADDAPDGLDNSLFSLLPAYALTEIKSAFKIGFYLYLPFLVVDMVVSS
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLALGMMMMSPVTISVPIKLILFVAMDGWTLICKGLIEQYLNLMQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ANNEIALIVLLTAATLVPFVVAAGSCFIKFSIVLVLVRNALGIQQVPSNLALNSIALIM
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SLFVMMPVAQSAYRYLQHHPLDVMSGASVNEFIDGGLGDYKRYLTRYSDPELVRFFERAQ
HHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
TARLKGDDADAADADDAPDGLDNSLFSLLPAYALTEIKSAFKIGFYLYLPFLVVDMVVSS
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLALGMMMMSPVTISVPIKLILFVAMDGWTLICKGLIEQYLNLMQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1312536; 8385666; 11115111; 11292750; 12384590 [H]