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The map label for this gene is pchA [H]

Identifier: 126442399

GI number: 126442399

Start: 845678

End: 847156

Strand: Reverse

Name: pchA [H]

Synonym: BURPS668_A0871

Alternate gene names: 126442399

Gene position: 847156-845678 (Counterclockwise)

Preceding gene: 126443288

Following gene: 126444667

Centisome position: 27.09

GC content: 74.44

Gene sequence:

>1479_bases
TTGGCGCCGCCAGCGGGGGCTCGCATGAGCGCCCGCCTGTCCGCGCTGCTCAATACGTTCGCGCGCGCCGCGCGCCGCGC
GGCCGACACGGGCGAGCCCGTGCTCGCCGCGGCATCGTTTCCGCTGCGGCCGCTGAACCTGTTCGATCTGATCGCGAGCT
GGGACGACGGCGCAACCCCATGGTGCTTCGTCGAAGCCGGCGACGAGCCGGGCGCGATGCGCGCGCCGGGCAAGCCACCC
GCCCTGTTCGGCTGGGATTGCGCACTCGATCTGAACGAGGCGGGCGACGCGCGATTCGCCCGGCTCGACGCGCGCTGGCG
CGCGCTCGTGCGCGCGGCGGTCGTCACCGGCGCGCAGCCGCCGCGCATCGTCGGCGGCTTCCGCTTCGATCCGCTCGGGC
CGCGATACGCGCACTGGCACGCGTTCGCCGATGCGAGCATGACGCTTGCGAAACTGCTGGTCGTCCGCGAAGGCGAGGCG
TATTGGGCGGTCTGCCAGCGCGTCGTCGCCGCGCACGACGATCCCGCCGCGCTCGCGCGCGAATGCGACGCGCGCATCGG
CATGCTCGCGTCGCTCGCGCCCGCCGCCGACGACGAGGCGCCGCGCCTGCTGCGCGTTCAGGCGCTGCAGGCCGCCGCAT
GGCAGCACGAGGTGCGGCGCGCGGTCGACGCGATCCGCGGCGAAGCGTTCAGCAAGGTCGTGCTCGCGCGCGACGTGCTG
CAACAGTACGCGCGGCCCGTGGCGATCGGGCCGCTGCTGCGCCGGCTCAGGCGGCGCGACCCGCGCGCGCACCTGTTCGC
GATCCGCAGGCGCGACGGCTGCTTCGTCGGCGCGACGCCGGAACGCCTCGCGCGCGTCGCGCACGGCCATGCGCACACGC
ACGCGCTCGCCGGCACGATCGCGCGCGGCAACGATCCGGCTCACGATCGCGCGCTCGGCGCGCAGCTGATGGCGTCGGCG
AAAGAGCGCCTCGAGCACGCGCTCGTGGTCGACGCGATTCGCGATGCGCTCGCGCCGCTGTCGCGCGTGATCGATGTGCC
CGCGCAGCCGTCGCTATTGCGGCTGCCGCGCCTGCAACATCTGAGCACGCCGATCGCCGCGGCGCTGAACGAGCGCGCGA
CGCTGCTGCAAGTCGTCGCGGCGCTGCATCCGACGCCCGCCGTCGCGGGCCATCCCCGCGCCGCCGCGCTCGAGCACATT
CGCGCGCACGAAGGCTTCGATCGCGGCTGGTATGCGGGCCCGATCGGCTGGATCGACGCGCACGGCAACGGCGACTTCGT
CGTCGCGCTGCGCTCGGCGCTGATCTCGGCCGGCGCCTGCCGGCTGTTCGCGGGCTGCGGGATCGTCGCCGAATCGGAGC
CGGCGCGCGAATATCGCGAAACGAGCCTCAAGCTGTCGGGGATGCAGGCGGCGATCGAGGCGCGCGACGCCGCAGCAGCA
GCGCGCACGGTCGCGCCGATTCCGTCCGCCGCGCACTGA

Upstream 100 bases:

>100_bases
ACCGGCGCCGCTGGGCCGAGCAGGCCGGGCTCGACGGCGCGTTCGTCGAGACGCTGTATGCGCAAATCATCGCGTGGTAC
ATCGAACAGCAAACGCGGCA

Downstream 100 bases:

>100_bases
CGAGGCATCGATGCGCCGCGCGCGCCGCGCGGCGCCGAATCGCGCCCAAACCGCGCCGCGCGGTGCGGCGCGCCACGCAT
CGCGCGACAAGCCGGGAAAA

Product: salicylate biosynthesis isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MAPPAGARMSARLSALLNTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPP
ALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEA
YWAVCQRVVAAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVL
QQYARPVAIGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGHAHTHALAGTIARGNDPAHDRALGAQLMASA
KERLEHALVVDAIRDALAPLSRVIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHI
RAHEGFDRGWYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAESEPAREYRETSLKLSGMQAAIEARDAAAA
ARTVAPIPSAAH

Sequences:

>Translated_492_residues
MAPPAGARMSARLSALLNTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPP
ALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEA
YWAVCQRVVAAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVL
QQYARPVAIGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGHAHTHALAGTIARGNDPAHDRALGAQLMASA
KERLEHALVVDAIRDALAPLSRVIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHI
RAHEGFDRGWYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAESEPAREYRETSLKLSGMQAAIEARDAAAA
ARTVAPIPSAAH
>Mature_491_residues
APPAGARMSARLSALLNTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATPWCFVEAGDEPGAMRAPGKPPA
LFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQPPRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAY
WAVCQRVVAAHDDPAALARECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVLQ
QYARPVAIGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGHAHTHALAGTIARGNDPAHDRALGAQLMASAK
ERLEHALVVDAIRDALAPLSRVIDVPAQPSLLRLPRLQHLSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIR
AHEGFDRGWYAGPIGWIDAHGNGDFVVALRSALISAGACRLFAGCGIVAESEPAREYRETSLKLSGMQAAIEARDAAAAA
RTVAPIPSAAH

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=260, Percent_Identity=38.0769230769231, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI87082077, Length=263, Percent_Identity=36.5019011406844, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788114, Length=208, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787518, Length=221, Percent_Identity=29.8642533936652, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=348, Percent_Identity=27.0114942528736, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52639; Mature: 52508

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPAGARMSARLSALLNTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
WCFVEAGDEPGAMRAPGKPPALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQP
EEEEECCCCCCCCCCCCCCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRVVAAHDDPAALAR
CCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
ECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQYARPVAIGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGHAHTHALAGTI
HHHCCCHHHHHHHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCCHHHHHHHHHH
ARGNDPAHDRALGAQLMASAKERLEHALVVDAIRDALAPLSRVIDVPAQPSLLRLPRLQH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
LSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDRGWYAGPIGWIDA
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
HGNGDFVVALRSALISAGACRLFAGCGIVAESEPAREYRETSLKLSGMQAAIEARDAAAA
CCCCCCHHHHHHHHHHHCHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTVAPIPSAAH
HHHHCCCCCCCC
>Mature Secondary Structure 
APPAGARMSARLSALLNTFARAARRAADTGEPVLAAASFPLRPLNLFDLIASWDDGATP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
WCFVEAGDEPGAMRAPGKPPALFGWDCALDLNEAGDARFARLDARWRALVRAAVVTGAQP
EEEEECCCCCCCCCCCCCCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PRIVGGFRFDPLGPRYAHWHAFADASMTLAKLLVVREGEAYWAVCQRVVAAHDDPAALAR
CCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
ECDARIGMLASLAPAADDEAPRLLRVQALQAAAWQHEVRRAVDAIRGEAFSKVVLARDVL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQYARPVAIGPLLRRLRRRDPRAHLFAIRRRDGCFVGATPERLARVAHGHAHTHALAGTI
HHHCCCHHHHHHHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCCHHHHHHHHHH
ARGNDPAHDRALGAQLMASAKERLEHALVVDAIRDALAPLSRVIDVPAQPSLLRLPRLQH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
LSTPIAAALNERATLLQVVAALHPTPAVAGHPRAAALEHIRAHEGFDRGWYAGPIGWIDA
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
HGNGDFVVALRSALISAGACRLFAGCGIVAESEPAREYRETSLKLSGMQAAIEARDAAAA
CCCCCCHHHHHHHHHHHCHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTVAPIPSAAH
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]