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The map label for this gene is ulaA [H]

Identifier: 162329624

GI number: 162329624

Start: 1931109

End: 1932914

Strand: Reverse

Name: ulaA [H]

Synonym: APL_1700

Alternate gene names: 162329624

Gene position: 1932914-1931109 (Counterclockwise)

Preceding gene: 126209169

Following gene: 126209163

Centisome position: 84.98

GC content: 43.69

Gene sequence:

>1806_bases
ATGGATACATTAATGAGTATCTTTATGGCAATTAATGACCAAATACTATCGAAAGCACCGCTATTGCTCGGTCTTGTCGC
TTGTATCGGTTATATATTCCTCAAAAAAGATTCAACCACCATTTTAAAAGGTACGATTAAAACGATTGTCGGCTTTATGT
TAGTACAAGTCGGTTCGGGTACGCTTGTACAAAACTTTAAACCGGTTATCGAAGGTTTATCCAAATACCATAATCTTAAA
GGTGCGGTAATTGACCCTTACACAAGCTGGCAAGCGACCATCAGTACCATGGGTGACGCTTATGCGTATGTCGGTTATGC
GGTAATCTTGGCATTATTCCTTAACATTTTGTTAGTTCTGTTCAGACACTTTACCGGTATTCGTACCATTATGCTTACCG
GTCATATTATGTTCCAACAAGCGGGCTTAATTGCGGTAGTATTCTGGCTACTCGGTTCAAAACCTTGGGAAGTGGTCGTT
TATACCGCAATTATCATGGCGTTATATTGGGGTATCTTCTCTAATATGATGTACCAAGCAACGCAACAAATTACCGGCGG
TGCGGGTTTCTCTATCGGTCACCAACAACAATTCGCCTCTTGGTTAGCGGCAAAAGTCGCACCGAAACTAGGTAAAAAAG
AAGAAAGCGTGGACAAACTCGACTTACCGAAATGGTTACATATTTTCCACGACAGTATCTCTGCAACCGCAATCGTGATG
ACCGTGTTCTTCGGTATTATCTTAATGTCATTCGGTTTAGATAACTTACAACAAATGGCGGGTAAAACCCATTGGACGAT
TTACATCTTTGAAACCGGCTTAAAATTTGCGGTGGCTATCCAAGTTATCGTATTAGGCGTACGTATGTTCGTTGCCGAAT
TATCCGAATCGTTCAAAGGGATTTCCGAACGCTTAATTCCGAATGCGGTGTTAGCGATTGACTGTGCGGCGACTTACGCA
TTCTCGCCAAATGCGGTAGTATTCGGTTTTATGTGGGGTGCTATCGGTCAATTCGTTGCGGTCGGCTTATTAATCGCTTA
TCAACAATTCGGCGGTGACGCTTCGGCGGCGGTGTTAATTATCCCGGGTTTTATCCCTATGTTCTTCTCTAACGCGACAA
TCGGCGTATTCGCAAACCACTTCGGCGGCTGGAAAGCGGTGATGAAAATCTGTTTCGTGATGGGTATCATCGAAGTGCTC
GGTTCCGCATGGGTAGTAAGCTTAATTACCGGCACTGAAGGCGGTGCGGGCTTTAACGGCTGGATGGGTATGGCGGACTG
GGCGTTATTATTCCCACCGATTATCCAAGGTATGTTCTTTAGCCCAGCGGGCGCAACCTTCGTATGTATCGCATTAGCCC
TTGTTTATATGGCGTTTGCCGGTAAACAATTACGTGCCGATGAAGCGAGAGCGAAAGCGGAAGGTAAAACCGTAGACCAA
TTATACGGCTTCGGCGAACCGATTGAAGAAGAAGTTCCGGCACAAGCGGAAACAGACAATACGCAAAGCGAAACCAAAGC
GGTACGTATTTTAGCGGTATGCGGTTCCGGTCAGGGTTCATCCATGATGATGAAAATGAAAATTAAACAATATCTTGATA
AACGCGGCGTACCGAATATTATGGACTCTTGCGCAGTAACCGATCATAAAGGTAAAGTCGGTTCGGTGGATATTATTGTA
TGTTCTAAACACTTACAAAATGAGATTGTGGCAAATGAACATATCTCGGTCTTAGGCGTACAAAATATGTTAAATCCGAA
TACCTTCGGTGAAGAGTTATTGGCTCTGATCAAAAAATATCAATAA

Upstream 100 bases:

>100_bases
AGATGTTTATGCCATTTTGTATTTAACTTAACCAATCTAAAAACACTGAGATCTTCCAAATCTCATCATTCTTACTCAAC
ATAGAACAGAGAGGCTCACT

Downstream 100 bases:

>100_bases
TCTGACATCCGCCTCTTAACGGAGGCGGGTTTATTTCCGATTAAATTAAACTTATCCAACTTAAAGGAAGCAAAGCTATG
TTAAAAGAATCGCTTATCGA

Product: PTS system ascorbate-specific transporter subunits IICB

Products: protein histidine; sugar phosphate

Alternate protein names: Ascorbate-specific PTS system EIIC component [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MDTLMSIFMAINDQILSKAPLLLGLVACIGYIFLKKDSTTILKGTIKTIVGFMLVQVGSGTLVQNFKPVIEGLSKYHNLK
GAVIDPYTSWQATISTMGDAYAYVGYAVILALFLNILLVLFRHFTGIRTIMLTGHIMFQQAGLIAVVFWLLGSKPWEVVV
YTAIIMALYWGIFSNMMYQATQQITGGAGFSIGHQQQFASWLAAKVAPKLGKKEESVDKLDLPKWLHIFHDSISATAIVM
TVFFGIILMSFGLDNLQQMAGKTHWTIYIFETGLKFAVAIQVIVLGVRMFVAELSESFKGISERLIPNAVLAIDCAATYA
FSPNAVVFGFMWGAIGQFVAVGLLIAYQQFGGDASAAVLIIPGFIPMFFSNATIGVFANHFGGWKAVMKICFVMGIIEVL
GSAWVVSLITGTEGGAGFNGWMGMADWALLFPPIIQGMFFSPAGATFVCIALALVYMAFAGKQLRADEARAKAEGKTVDQ
LYGFGEPIEEEVPAQAETDNTQSETKAVRILAVCGSGQGSSMMMKMKIKQYLDKRGVPNIMDSCAVTDHKGKVGSVDIIV
CSKHLQNEIVANEHISVLGVQNMLNPNTFGEELLALIKKYQ

Sequences:

>Translated_601_residues
MDTLMSIFMAINDQILSKAPLLLGLVACIGYIFLKKDSTTILKGTIKTIVGFMLVQVGSGTLVQNFKPVIEGLSKYHNLK
GAVIDPYTSWQATISTMGDAYAYVGYAVILALFLNILLVLFRHFTGIRTIMLTGHIMFQQAGLIAVVFWLLGSKPWEVVV
YTAIIMALYWGIFSNMMYQATQQITGGAGFSIGHQQQFASWLAAKVAPKLGKKEESVDKLDLPKWLHIFHDSISATAIVM
TVFFGIILMSFGLDNLQQMAGKTHWTIYIFETGLKFAVAIQVIVLGVRMFVAELSESFKGISERLIPNAVLAIDCAATYA
FSPNAVVFGFMWGAIGQFVAVGLLIAYQQFGGDASAAVLIIPGFIPMFFSNATIGVFANHFGGWKAVMKICFVMGIIEVL
GSAWVVSLITGTEGGAGFNGWMGMADWALLFPPIIQGMFFSPAGATFVCIALALVYMAFAGKQLRADEARAKAEGKTVDQ
LYGFGEPIEEEVPAQAETDNTQSETKAVRILAVCGSGQGSSMMMKMKIKQYLDKRGVPNIMDSCAVTDHKGKVGSVDIIV
CSKHLQNEIVANEHISVLGVQNMLNPNTFGEELLALIKKYQ
>Mature_601_residues
MDTLMSIFMAINDQILSKAPLLLGLVACIGYIFLKKDSTTILKGTIKTIVGFMLVQVGSGTLVQNFKPVIEGLSKYHNLK
GAVIDPYTSWQATISTMGDAYAYVGYAVILALFLNILLVLFRHFTGIRTIMLTGHIMFQQAGLIAVVFWLLGSKPWEVVV
YTAIIMALYWGIFSNMMYQATQQITGGAGFSIGHQQQFASWLAAKVAPKLGKKEESVDKLDLPKWLHIFHDSISATAIVM
TVFFGIILMSFGLDNLQQMAGKTHWTIYIFETGLKFAVAIQVIVLGVRMFVAELSESFKGISERLIPNAVLAIDCAATYA
FSPNAVVFGFMWGAIGQFVAVGLLIAYQQFGGDASAAVLIIPGFIPMFFSNATIGVFANHFGGWKAVMKICFVMGIIEVL
GSAWVVSLITGTEGGAGFNGWMGMADWALLFPPIIQGMFFSPAGATFVCIALALVYMAFAGKQLRADEARAKAEGKTVDQ
LYGFGEPIEEEVPAQAETDNTQSETKAVRILAVCGSGQGSSMMMKMKIKQYLDKRGVPNIMDSCAVTDHKGKVGSVDIIV
CSKHLQNEIVANEHISVLGVQNMLNPNTFGEELLALIKKYQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active- transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3037

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaA family [H]

Homologues:

Organism=Escherichia coli, GI87082388, Length=480, Percent_Identity=61.25, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1790638, Length=94, Percent_Identity=47.8723404255319, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: 2.7.1.69

Molecular weight: Translated: 65390; Mature: 65390

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLMSIFMAINDQILSKAPLLLGLVACIGYIFLKKDSTTILKGTIKTIVGFMLVQVGSG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVQNFKPVIEGLSKYHNLKGAVIDPYTSWQATISTMGDAYAYVGYAVILALFLNILLVL
HHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRHFTGIRTIMLTGHIMFQQAGLIAVVFWLLGSKPWEVVVYTAIIMALYWGIFSNMMYQA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TQQITGGAGFSIGHQQQFASWLAAKVAPKLGKKEESVDKLDLPKWLHIFHDSISATAIVM
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TVFFGIILMSFGLDNLQQMAGKTHWTIYIFETGLKFAVAIQVIVLGVRMFVAELSESFKG
HHHHHHHHHHHCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERLIPNAVLAIDCAATYAFSPNAVVFGFMWGAIGQFVAVGLLIAYQQFGGDASAAVLI
HHHHHCCCHHEEEEHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IPGFIPMFFSNATIGVFANHFGGWKAVMKICFVMGIIEVLGSAWVVSLITGTEGGAGFNG
ECCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
WMGMADWALLFPPIIQGMFFSPAGATFVCIALALVYMAFAGKQLRADEARAKAEGKTVDQ
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LYGFGEPIEEEVPAQAETDNTQSETKAVRILAVCGSGQGSSMMMKMKIKQYLDKRGVPNI
HHCCCCCHHHHCCCCCCCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHH
MDSCAVTDHKGKVGSVDIIVCSKHLQNEIVANEHISVLGVQNMLNPNTFGEELLALIKKY
HHHCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEHHHHCCCCCHHHHHHHHHHHC
Q
C
>Mature Secondary Structure
MDTLMSIFMAINDQILSKAPLLLGLVACIGYIFLKKDSTTILKGTIKTIVGFMLVQVGSG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVQNFKPVIEGLSKYHNLKGAVIDPYTSWQATISTMGDAYAYVGYAVILALFLNILLVL
HHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRHFTGIRTIMLTGHIMFQQAGLIAVVFWLLGSKPWEVVVYTAIIMALYWGIFSNMMYQA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TQQITGGAGFSIGHQQQFASWLAAKVAPKLGKKEESVDKLDLPKWLHIFHDSISATAIVM
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TVFFGIILMSFGLDNLQQMAGKTHWTIYIFETGLKFAVAIQVIVLGVRMFVAELSESFKG
HHHHHHHHHHHCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERLIPNAVLAIDCAATYAFSPNAVVFGFMWGAIGQFVAVGLLIAYQQFGGDASAAVLI
HHHHHCCCHHEEEEHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IPGFIPMFFSNATIGVFANHFGGWKAVMKICFVMGIIEVLGSAWVVSLITGTEGGAGFNG
ECCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
WMGMADWALLFPPIIQGMFFSPAGATFVCIALALVYMAFAGKQLRADEARAKAEGKTVDQ
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LYGFGEPIEEEVPAQAETDNTQSETKAVRILAVCGSGQGSSMMMKMKIKQYLDKRGVPNI
HHCCCCCHHHHCCCCCCCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHH
MDSCAVTDHKGKVGSVDIIVCSKHLQNEIVANEHISVLGVQNMLNPNTFGEELLALIKKY
HHHCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEHHHHCCCCCHHHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]