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The map label for this gene is ldhA [C]

Identifier: 126209493

GI number: 126209493

Start: 2266593

End: 2267543

Strand: Reverse

Name: ldhA [C]

Synonym: APL_2039

Alternate gene names: 126209493

Gene position: 2267543-2266593 (Counterclockwise)

Preceding gene: 126209494

Following gene: 126209492

Centisome position: 99.69

GC content: 44.16

Gene sequence:

>951_bases
ATGACTGTGCTTAATATCGTTTTTCTAGATAAAACCGGTGTACCCACCCACGAAATTCCTCGTCCTTCATTTCCTCATAT
TTGGACTGAATACGACAGTACCGATGCTTCACAAACCTTTGAGCGTGCCAAAGATGCCGATATCATCGTGACCAGCAAAG
TATTACTTGGCAGAGAATTACTGGCTCAATTACCGAAACTTAAATTAATTGCAATTACCGCCACCGGTACCAATAATGTT
GATTTAGTCGCGGCGAAAGAATTAGGTATTGCAGTAAAAAACGTGACCGGCTATTCCACCACAACCGTGCCGGAACACGT
TTTAGGTATGATTTTTGCGTTAAAGCACAGCTTAATCGGTTATCATCGTGATCAAATCACCTCGGATCGTTGGGCAACTT
GCGGACAATTCTGCTATGTCGATCATCCGGTAACCGATATTCGCGGCTCAACCTTAGCTATTTTCGGTAAAGGCAACACC
GGTTCGGAAGTGGCTCGCTTAGCACAATCGGTCGGAATGAAAGTCATTTTTGCGGAACACAAAGGTGCAACTGAAATTCG
TGAAGGTTATACCGCTTTTGAAGAAGTGTTTAAACAAGCGGATATTATTTCATTACATTGCCCGCTCACCGATTCAACTC
AAAACCTGATTAACGCCGAAACCTTGGCATTAATGAAACCGACCGCTTATTTAATTAATACCGGCAGAGGCCCGTTAGTG
GATGAAGCGGCATTATTAGCGGCTTTAGAACAGGGCAAGATTGCCGGTGCGGCGCTGGACGTATTAGTCAAAGAACCGCC
GGAAAAAGACAATCCGTTAATGCAGGCGGCAAAACGTTTACCGAACTTATTAATTACCCCGCACGTTGCGTGGGCAAGCG
ATTCTGCAGTCACCACTTTAGTAAATAAAGTGACGCAGAATATGGAAGAATTTGTTCTCAACGGGAAATAA

Upstream 100 bases:

>100_bases
GATGATTTAGTTAAATCATTTGAAGAAATTGATACATCAAACTAATGCCTATTACCACGAGGAGTCATCACTTCTCGTGG
TAAAAATTTAAGGAGCCTCT

Downstream 100 bases:

>100_bases
TTTTCTACGAAATAGCCTCATTTCCCAAAGACACAAGCGGTTTAAAATTAGCGAAAATTTGCAAAAAATTCGGTAAATTT
GACCGCTTGCTTTTGTATTG

Product: glycerate dehydrogenase

Products: 3-Phosphohydroxypyruvate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTVLNIVFLDKTGVPTHEIPRPSFPHIWTEYDSTDASQTFERAKDADIIVTSKVLLGRELLAQLPKLKLIAITATGTNNV
DLVAAKELGIAVKNVTGYSTTTVPEHVLGMIFALKHSLIGYHRDQITSDRWATCGQFCYVDHPVTDIRGSTLAIFGKGNT
GSEVARLAQSVGMKVIFAEHKGATEIREGYTAFEEVFKQADIISLHCPLTDSTQNLINAETLALMKPTAYLINTGRGPLV
DEAALLAALEQGKIAGAALDVLVKEPPEKDNPLMQAAKRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVLNGK

Sequences:

>Translated_316_residues
MTVLNIVFLDKTGVPTHEIPRPSFPHIWTEYDSTDASQTFERAKDADIIVTSKVLLGRELLAQLPKLKLIAITATGTNNV
DLVAAKELGIAVKNVTGYSTTTVPEHVLGMIFALKHSLIGYHRDQITSDRWATCGQFCYVDHPVTDIRGSTLAIFGKGNT
GSEVARLAQSVGMKVIFAEHKGATEIREGYTAFEEVFKQADIISLHCPLTDSTQNLINAETLALMKPTAYLINTGRGPLV
DEAALLAALEQGKIAGAALDVLVKEPPEKDNPLMQAAKRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVLNGK
>Mature_315_residues
TVLNIVFLDKTGVPTHEIPRPSFPHIWTEYDSTDASQTFERAKDADIIVTSKVLLGRELLAQLPKLKLIAITATGTNNVD
LVAAKELGIAVKNVTGYSTTTVPEHVLGMIFALKHSLIGYHRDQITSDRWATCGQFCYVDHPVTDIRGSTLAIFGKGNTG
SEVARLAQSVGMKVIFAEHKGATEIREGYTAFEEVFKQADIISLHCPLTDSTQNLINAETLALMKPTAYLINTGRGPLVD
EAALLAALEQGKIAGAALDVLVKEPPEKDNPLMQAAKRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVLNGK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=250, Percent_Identity=36, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4557499, Length=250, Percent_Identity=36, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI61743967, Length=265, Percent_Identity=32.8301886792453, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI4557497, Length=265, Percent_Identity=32.8301886792453, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580575, Length=250, Percent_Identity=36, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI23308577, Length=253, Percent_Identity=33.201581027668, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI6912396, Length=247, Percent_Identity=27.5303643724696, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789279, Length=299, Percent_Identity=31.1036789297659, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787645, Length=266, Percent_Identity=33.0827067669173, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082289, Length=294, Percent_Identity=30.2721088435374, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI87081824, Length=173, Percent_Identity=31.7919075144509, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788660, Length=228, Percent_Identity=28.0701754385965, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=276, Percent_Identity=32.6086956521739, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17532191, Length=273, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=323, Percent_Identity=28.7925696594427, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320925, Length=290, Percent_Identity=30, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324964, Length=251, Percent_Identity=29.4820717131474, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=266, Percent_Identity=31.203007518797, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325144, Length=123, Percent_Identity=35.7723577235772, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=91, Percent_Identity=34.0659340659341, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646446, Length=256, Percent_Identity=37.109375, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646448, Length=256, Percent_Identity=37.109375, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646452, Length=256, Percent_Identity=37.109375, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646450, Length=256, Percent_Identity=37.109375, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI62472511, Length=256, Percent_Identity=37.109375, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921140, Length=281, Percent_Identity=32.0284697508897, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574286, Length=268, Percent_Identity=29.4776119402985, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28571528, Length=239, Percent_Identity=29.2887029288703, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585516, Length=230, Percent_Identity=26.9565217391304, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552429, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585514, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574282, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45551003, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574284, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95 [C]

Molecular weight: Translated: 34313; Mature: 34182

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLNIVFLDKTGVPTHEIPRPSFPHIWTEYDSTDASQTFERAKDADIIVTSKVLLGREL
CEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHH
LAQLPKLKLIAITATGTNNVDLVAAKELGIAVKNVTGYSTTTVPEHVLGMIFALKHSLIG
HHHCCCEEEEEEEECCCCCEEEEEEHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YHRDQITSDRWATCGQFCYVDHPVTDIRGSTLAIFGKGNTGSEVARLAQSVGMKVIFAEH
HHHHHCCCCHHHHCCCEEEECCCHHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECC
KGATEIREGYTAFEEVFKQADIISLHCPLTDSTQNLINAETLALMKPTAYLINTGRGPLV
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCH
DEAALLAALEQGKIAGAALDVLVKEPPEKDNPLMQAAKRLPNLLITPHVAWASDSAVTTL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCEECCCCHHHHHH
VNKVTQNMEEFVLNGK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVLNIVFLDKTGVPTHEIPRPSFPHIWTEYDSTDASQTFERAKDADIIVTSKVLLGREL
EEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHH
LAQLPKLKLIAITATGTNNVDLVAAKELGIAVKNVTGYSTTTVPEHVLGMIFALKHSLIG
HHHCCCEEEEEEEECCCCCEEEEEEHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YHRDQITSDRWATCGQFCYVDHPVTDIRGSTLAIFGKGNTGSEVARLAQSVGMKVIFAEH
HHHHHCCCCHHHHCCCEEEECCCHHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECC
KGATEIREGYTAFEEVFKQADIISLHCPLTDSTQNLINAETLALMKPTAYLINTGRGPLV
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCH
DEAALLAALEQGKIAGAALDVLVKEPPEKDNPLMQAAKRLPNLLITPHVAWASDSAVTTL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCEECCCCHHHHHH
VNKVTQNMEEFVLNGK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-Phospho-D-glycerate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 3-Phospho-D-glycerate + Nicotinamide adenine dinucleotide --> 3-Phosphohydroxypyruvate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]